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NET1 (NM_005863) Human Tagged ORF Clone
CAT＃: RC201682
NET1 (Myc-DDK-tagged)-Human neuroepithelial cell transforming 1 (NET1), transcript variant 2
ORF Plasmid: tGFP
"NM_005863" in other vectors (6)
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CNY 4,040.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARHGEF8; NET1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201682 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCACATGATGAGACTGGAGGTCTCCTACCTATTAAAAGGACCATACGAGTCCTAGATGTCAATA ACCAGTCCTTCAGAGAACAAGAGGAGCCAAGCAATAAAAGAGTTCGACCTCTGGCTCGTGTCACGTCCTT GGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGTTTTGGTCAAACAATACAGTCATTTACC CTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTTTCTAGCAGGTCAACAGTCCCAACACCCG CCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTGGACATCACCATGAAGGAGTCTCTCACCACCAG GGAGATCAGACGGCAGGAGGCAATATATGAAATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAA CTTGCAAGAAAGGCCTACCATGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAACTCACACATA TATTTGGTGATCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACAAGAATAGGAGAAGCAACCAA GCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTGAGCTGGTTACCGCGCTTGAATGCCTACAGA GGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTTCTTGATCAAAAGAAACAGGATCCAAGAGTCCAAG ACTTCCTCCAGCGATGTCTCGAGTCTCCCTTCAGTCGAAAACTAGATCTTTGGAGTTTCCTAGATATCCC TCGAAGTCGCCTAGTCAAATACCCTTTACTGTTAAAAGAAATTCTTAAACACACTCCAAAAGAGCACCCT GATGTTCAGCTTCTGGAGGATGCTATATTGATAATACAGGGAGTCCTCTCTGATATCAACTTGAAGAAAG GTGAATCCGAGTGCCAGTATTACATCGACAAGCTGGAGTACCTGGATGAAAAGCAGAGGGACCCCAGAAT CGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGCGGAGCAAGAGTGGACATAAACTTTACATTTTC CTGTTTCAAGACATCTTGGTTCTGACTCGGCCCGTCACACGGAACGAACGGCACTCTTACCAGGTTTACC GGCAGCCAATCCCAGTCCAAGAGCTAGTCCTAGAAGACCTGCAGGATGGAGATGTGAGAATGGGAGGCTC CTTTCGAGGAGCTTTCAGTAACTCAGAGAAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCT CCAGCCCAGTCTCACACTCTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTGGTTCAACTGTATTCGAG CGGCCATTGCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCTGCAGGGCCTGCCGGAGCTGCACGAAGA GTGTGAGGGGAACCACCCCTCTGCGAGGAAACTCACAGCCCAGAGGAGGGCATCCACAGTTTCCAGTGTT ACTCAGGTAGAAGTTGATGAAAACGCTTACAGATGTGGCTCTGGCATGCAGATGGCAGAGGACAGCAAGA GCTTAAAGACACACCAGACACAGCCCGGCATCCGAAGAGCGAGGGACAAAGCCCTTTCTGGTGGCAAACG GAAAGAGACTTTGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201682 protein sequence
Red=Cloning site Green=Tags(s) MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFT LRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLK LARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYR GYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHP DVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIF LFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPS PAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSV TQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005863 |
| ORF Size | 1626 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005863.4 |
| RefSeq Size | 3906 bp |
| RefSeq ORF | 1629 bp |
| Locus ID | 10276 |
| UniProt ID | Q7Z628 |
| Domains | RhoGEF, PH |
| Protein Families | Druggable Genome |
| MW | 61.9 kDa |
| Gene Summary | This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201682L1 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, Myc-DDK-tagged |
CNY 6,440.00 |
|
| RC201682L2 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC201682L3 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201682L4 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201682 | NET1 (tGFP-tagged) - Human neuroepithelial cell transforming 1 (NET1), transcript variant 2 |
CNY 5,640.00 |
|
| SC116508 | NET1 (untagged)-Human neuroepithelial cell transforming 1 (NET1), transcript variant 2 |
CNY 4,040.00 |
