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NET1 (NM_005863) Human Untagged Clone
CAT＃: SC116508
NET1 (untagged)-Human neuroepithelial cell transforming 1 (NET1), transcript variant 2
CNY 4,040.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARHGEF8; NET1A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116508 sequence for NM_005863 edited (data generated by NextGen Sequencing)
ATGGTGGCACATGATGAGACTGGAGGTCTCCTACCTATTAAAAGGACCATACGAGTCCTA GATGTCAATAACCAGTCCTTCAGAGAACAAGAGGAGCCAAGCAATAAAAGAGTTCGACCT CTGGCTCGTGTCACGTCCTTGGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGA CGTTTTGGTCAAACAATACAGTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCT GCCCAGAAGTTTTCTAGCAGGTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCA CTGTGGTCAGAGATGCTGGACATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGA CGGCAGGAGGCAATATATGAAATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAA CTTGCAAGAAAGGCCTACCATGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAA CTCACACATATATTTGGTGATCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACA AGAATAGGAGAAGCAACCAAGCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTG AGCTGGTTACCGCGCTTGAATGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAA GCTCTTCTTGATCAAAAGAAACAGGATCCAAGAGTCCAAGACTTCCTCCAGCGATGTCTC GAGTCTCCCTTCAGTCGAAAACTAGATCTTTGGAGTTTCCTAGATATCCCTCGAAGTCGC CTAGTCAAATACCCTTTACTGTTAAAAGAAATTCTTAAACACACTCCAAAAGAGCACCCT GATGTTCAGCTTCTGGAGGATGCTATATTGATAATACAGGGAGTCCTCTCTGATATCAAC TTGAAGAAAGGTGAATCCGAGTGCCAGTATTACATCGACAAGCTGGAGTACCTGGATGAA AAGCAGAGGGACCCCAGAATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGCGG AGCAAGAGTGGACATAAACTTTACATTTTCCTGTTTCAAGACATCTTGGTTCTGACTCGG CCCGTCACACGGAACGAACGGCACTCTTACCAGGTTTACCGGCAGCCAATCCCAGTCCAA GAGCTAGTCCTAGAAGACCTGCAGGATGGAGATGTGAGAATGGGAGGCTCCTTTCGAGGA GCTTTCAGTAACTCAGAGAAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCT CCAGCCCAGTCTCACACTCTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTGGTTCAAC TGTATTCGAGCGGCCATTGCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCTGCAGGGC CTGCCGGAGCTGCACGAAGAGTGTGAGGGGAACCACCCCTCTGCGAGGAAACTCACAGCC CAGAGGAGGGCATCCACAGTTTCCAGTGTTACTCAGGTAGAAGTTGATGAAAACGCTTAC AGATGTGGCTCTGGCATGCAGATGGCAGAGGACAGCAAGAGCTTAAAGACACACCAGACA CAGCCCGGCATCCGAAGAGCGAGGGACAAAGCCCTTTCTGGTGGCAAACGGAAAGAGACT TTGGTGTAG Clone variation with respect to NM_005863.3 >OriGene 5' read for NM_005863 unedited
GAAAACCCATTCGGCACGAGGCCGCGGCGATAGGCATGGGCACGTGGCTGCCGAGGTGGC CGAGCTCTGGGAAGAAAAGCCCGTGTGCCTCTGCATAGCGTCGCTACAGCGCTGACTCGG TGTGGATTGATTGGAAAGGTTTGAGGGAGTACTTGGGAAGCATGGTGGCACATGATGATA TTGTTGTCTCCTACCTATTAAAAGGACCATACGAGTCCTAGATGTCAATAACCAGTCCTT CAGAGAACAAGAGGAGCCAAGCAATAAAAGAGTTCGACCTCTGGCTCGTGTCACGTCCTT GGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGTTTTGGTCAAACAATACA GTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTTTCTAGCAG GTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTGGA CATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGACGGCAGGAGGCAATATATGA AATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAACTTGCAAGAAAGGCCTACCA TGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAACTCACACATATATTTGGTGA TCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACNAGAATAGGAGAAGCAACCAA GCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTGAGCTGGTTACCGCGCTTTGA TGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTTTCTGATCAANNAGA ACAGATCCAAGAGTCCAAGACTTCCTCCAGCA >OriGene 3' read for NM_005863 unedited
TAGAGTCGAGTTTTTTTTTTTTTTTTTTACACACACATTTTTAATCAGGAAGAAAAATAC TTTAAAGACATGCCAATTTGAAAAGGCATCAAAGTAAAAAAATAAAAGCAAATGCTAAAA ACTACTTTACAATAAAAAAATTAAATAATCGGCAGGTTAAATGAATGTAAAATGAGGAAT GTACAGTGAAAAACAAACTAATATAAAGCATTCCAGTTGATAAAAACCTCCTCAGGCTTA TGGTTTGTTTTCCAAGGAAATTATGTTTCAATGTAAAGTTTGAAATACTCCAGACATACA TTCCATGTAGGTTTTGGGTGCCAATGTTAAAATTTCAAATTTTGCATGCAAGGCTTAGCA AAGAAACACTGGCAGAATTCCAGCATTTGCAAAATTCTAAGTTTTGGTGAATATTGTAAA TATTACAATTGGTATTAGAAAGCCATGATGAATCCAGAATTAAGAGAAAACCCATTTCAT AAATATTTTGTTTGATTAAAAAATACCAGGCTTACCATGTTCTAAATAATTCAAGAAAAA TATCTTTAAAAAAAAAGGGACTGCATTTACAGTAATCTGTATATCTTTAGCTGCCATTAA AAAAAGAANAAAGAACACCCANAAACANTGAAAATGTTACAACTGGTATAAAGTAACCCT ATGATGCTNCCCTTACGAGAAAACAAACTGTACACATTTATAAACAAACAGTCTCTCCCA TCAGTTTACACACAGAGCCTTTCTCTACACAAAGTCTCTTTCCGTTTTGCACAGAAAAGG GCTTGTCCNTCGCTCTTCGGATGCCNGGCTGGGTCTGGNGNGCCTTAAGCTTTTGCTGCC TTTGCATTNGATGCAGAGCCCATTGGAAACGTTTTATACTTCTCCTGAGTACCTGGAACT GGGATGCCTCTTTGGCTGGAGTTTCTGCAAAGGGGGGTCCCCTAATTTTTTGCACTCCGA GCCTGATTTAGTGACTCCTCCCATGAGGGCATGCCNTTAATCATGACCTGTGGTGGAACC T |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005863 |
| Insert Size | 2620 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005863.2, NP_005854.2 |
| RefSeq Size | 3236 bp |
| RefSeq ORF | 1629 bp |
| Locus ID | 10276 |
| UniProt ID | Q7Z628 |
| Domains | RhoGEF, PH |
| Protein Families | Druggable Genome |
| Gene Summary | This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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The nuclear RhoA-exchange factor Net1 interacts with proteins of the Dlg family, affects their localization and influences their tumor suppressor activity
,Garcia-Mata R, Dubash AD, Sharek L, Carr HS, Frost JA, Burridge K.,
Mol Cell Biol. 2007 Dec;27(24):8683-97.
[NET1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201682 | NET1 (Myc-DDK-tagged)-Human neuroepithelial cell transforming 1 (NET1), transcript variant 2 |
CNY 4,040.00 |
|
| RC201682L1 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, Myc-DDK-tagged |
CNY 6,440.00 |
|
| RC201682L2 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC201682L3 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201682L4 | Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201682 | NET1 (tGFP-tagged) - Human neuroepithelial cell transforming 1 (NET1), transcript variant 2 |
CNY 5,640.00 |
