KRAS (NM_004985) Human Tagged ORF Clone

CAT＃: RC201958

TrueORF^®

KRAS (Myc-DDK-tagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_004985" in other vectors (6)

Need custom modification / cloning service?
Get a free quote

CNY 3,600.00

CNY 3,705.00

货期*

现货

规格

Cited in 10 publications.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA801672

KRAS mouse monoclonal antibody, clone OTI2C1 (formerly 2C1)

CNY 1,999.00
CNY 2,700.00

热销推荐

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC201958 representing NM_004985 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACAGCTAA TTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAGGATTCCTACAGGAAGCAAGTAGTAATTGA TGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAG TACATGAGGACTGGGGAGGGCTTTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTC ACCATTATAGAGAACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAA ATGTGATTTGCCTTCTAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATGGAATTCCT TTTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTATACATTAGTTCGAGAAATTC GAAAACATAAAGAAAAGATGAGCAAAGATGGTAAAAAGAAGAAAAAGAAGTCAAAGACAAAGTGTGTAAT TATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC201958 representing NM_004985 Red=Cloning site Green=Tags(s) MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQ YMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIP FIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_004985
ORF Size	564 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_004985.5
RefSeq Size	5312 bp
RefSeq ORF	567 bp
Locus ID	3845
UniProt ID	P01116
Domains	ras, RAS, RHO, RAB
Protein Families	Druggable Genome
Protein Pathways	Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway
MW	21.2 kDa
Gene Summary	This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (10)

The use of this cDNA Clones has been cited in the following citations:
Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer ,Woolston, A;Khan, K;Spain, G;Barber, LJ;Griffiths, B;Gonzalez-Exposito, R;Hornsteiner, L;Punta, M;Patil, Y;Newey, A;Mansukhani, S;Davies, MN;Furness, A;Sclafani, F;Peckitt, C;Jiménez, M;Kouvelakis, K;Ranftl, R;Begum, R;Rana, I;Thomas, J;Bryant, A;Quezada, S;Wotherspoon, A;Khan, N;Fotiadis, N;Marafioti, T;Powles, T;Lise, S;Calvo, F;Guettler, S;von Loga, K;Rao, S;Watkins, D;Starling, N;Chau, I;Sadanandam, A;Cunningham, D;Gerlinger, M;, Cancer Cell ,PubMed ID 31287991 [KRAS]
KRAS G12D or G12V mutation in human brain arteriovenous malformations ,Oka, M;Kushamae, M;Aoki, T;Yamaguchi, T;Kitazato, K;Abekura, Y;Kawamata, T;Mizutani, T;Miyamoto, S;Takagi, Y;, World Neurosurg ,PubMed ID 30902772 [KRAS]
High expression of RAD51 promotes DNA damage repair and survival in KRAS-mutant lung cancer cells ,Hu, J;Zhang, Z;Zhao, L;Li, L;Zuo, W;Han, L;, BMB Rep ,PubMed ID 30638176 [KRAS]
Characterizing the interaction between insulin-like growth factor 2 mRNA-binding protein 1 (IMP1) and KRAS expression ,Mackedenski, S;Wang, C;Li, WM;Lee, CH;, Biochem. J. ,PubMed ID 30104206 [KRAS]
Mutant KRAS promotes liver metastasis of colorectal cancer, in part, by upregulating the MEK-Sp1-DNMT1-miR-137-YB-1-IGF-IR signaling pathway ,Chu, PC;Lin, PC;Wu, HY;Lin, KT;Wu, C;Bekaii-Saab, T;Lin, YJ;Lee, CT;Lee, JC;Chen, CS;, Oncogene ,PubMed ID 29559746 [KRAS]
MicroRNA-622 is a novel mediator of tumorigenicity in melanoma by targeting Kirsten rat sarcoma ,Dietrich, P;Kuphal, S;Spruss, T;Hellerbrand, C;Bosserhoff, AK;, Pigment Cell Melanoma Res ,PubMed ID 29495114 [KRAS]
KRAS induces lung tumorigenesis through microRNAs modulation ,Shi, L;Middleton, J;Jeon, YJ;Magee, P;Veneziano, D;Laganà, A;Leong, HS;Sahoo, S;Fassan, M;Booton, R;Shah, R;Crosbie, PAJ;Garofalo, M;, Cell Death Dis ,PubMed ID 29440633 [KRAS]
Gene Silencing Using Multifunctionalized Gold Nanoparticles for Cancer Therapy ,Fernandes, AR;Baptista, PV;, Methods Mol. Biol ,PubMed ID 28150211 [KRAS]
Metabolic Adaptation to Nutritional Stress in Human Colorectal Cancer ,Miyo, M;Konno, M;Nishida, N;Sueda, T;Noguchi, K;Matsui, H;Colvin, H;Kawamoto, K;Koseki, J;Haraguchi, N;Nishimura, J;Hata, T;Gotoh, N;Matsuda, F;Satoh, T;Mizushima, T;Shimizu, H;Doki, Y;Mori, M;Ishii, H;, Sci Rep ,PubMed ID 27924922 [KRAS]
MiR-124 governs glioma growth and angiogenesis and enhances chemosensitivity by targeting R-Ras and N-Ras ,Shi, Z;Chen, Q;Li, C;Wang, L;Qian, X;Jiang, C;Liu, X;Wang, X;Li, H;Kang, C;Jiang, T;Liu, LZ;You, Y;Liu, N;Jiang, BH;, Neuro-oncology ,PubMed ID 24861879 [KRAS]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC201958L1	Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,000.00
RC201958L2	Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,000.00
RC201958L3	Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,000.00
RC201958L4	Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,000.00
RG201958	KRAS (tGFP-tagged) - Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,200.00
SC109374	KRAS (untagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00