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CLN8 (NM_018941) Human Tagged ORF Clone
CAT＃: RC202936
CLN8 (Myc-DDK-tagged)-Human ceroid-lipofuscinosis, neuronal 8 (epilepsy, progressive with mental retardation) (CLN8)
ORF Plasmid: tGFP
"NM_018941" in other vectors (5)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C8orf61; EPMR; TLCD6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202936 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCTGCGAGCGATGGGGGCACATCAGAGAGCATTTTTGACCTGGACTATGCATCCTGGGGGATCC GCTCCACGCTGATGGTCGCTGGCTTTGTCTTCTACTTGGGCGTCTTTGTGGTCTGCCACCAGCTGTCCTC TTCCCTGAATGCCACTTACCGTTCTTTGGTGGCCAGAGAGAAGGTCTTCTGGGACCTGGCGGCCACGCGT GCAGTCTTTGGTGTTCAGAGCACAGCCGCAGGCCTGTGGGCTCTGCTGGGGGACCCTGTGCTGCATGCCG ACAAGGCGCGTGGCCAGCAGAACTGGTGCTGGTTTCACATCACGACAGCAACGGGATTCTTTTGCTTTGA AAATGTTGCAGTCCACCTGTCCAACTTGATCTTCCGGACATTTGACTTGTTTCTGGTTATCCACCATCTC TTTGCCTTTCTTGGGTTTCTTGGCTGCTTGGTCAATCTCCAAGCTGGCCACTATCTAGCTATGACCACGT TGCTCCTGGAGATGAGCACGCCCTTTACCTGCGTTTCCTGGATGCTCTTAAAGGCGGGCTGGTCCGAGTC TCTGTTTTGGAAGCTCAACCAGTGGCTGATGATTCACATGTTTCACTGCCGCATGGTTCTAACCTACCAC ATGTGGTGGGTGTGTTTCTGGCACTGGGACGGCCTGGTCAGCAGCCTGTATCTGCCTCATTTGACACTGT TCCTTGTCGGACTGGCTCTGCTTACGCTAATCATTAATCCATATTGGACCCATAAGAAGACTCAGCAGCT TCTCAATCCGGTGGACTGGAACTTCGCACAGCCAGAAGCCAAGAGCAGGCCAGAAGGCAACGGGCAGCTG CTGCGGAAGAAGAGGCCA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC202936 protein sequence
Red=Cloning site Green=Tags(s) MNPASDGGTSESIFDLDYASWGIRSTLMVAGFVFYLGVFVVCHQLSSSLNATYRSLVAREKVFWDLAATR AVFGVQSTAAGLWALLGDPVLHADKARGQQNWCWFHITTATGFFCFENVAVHLSNLIFRTFDLFLVIHHL FAFLGFLGCLVNLQAGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSESLFWKLNQWLMIHMFHCRMVLTYH MWWVCFWHWDGLVSSLYLPHLTLFLVGLALLTLIINPYWTHKKTQQLLNPVDWNFAQPEAKSRPEGNGQL LRKKRP SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018941 |
| ORF Size | 858 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018941.4 |
| RefSeq Size | 7185 bp |
| RefSeq ORF | 861 bp |
| Locus ID | 2055 |
| UniProt ID | Q9UBY8 |
| Domains | TLC |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 32.8 kDa |
| Gene Summary | This gene encodes a transmembrane protein belonging to a family of proteins containing TLC domains, which are postulated to function in lipid synthesis, transport, or sensing. The protein localizes to the endoplasmic reticulum (ER), and may recycle between the ER and ER-Golgi intermediate compartment. Mutations in this gene are associated with a disorder characterized by progressive epilepsy with cognitive disabilities (EPMR), which is a subtype of neuronal ceroid lipofuscinoses (NCL). Patients with mutations in this gene have altered levels of sphingolipid and phospholipids in the brain. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202936L3 | Lenti ORF clone of Human ceroid-lipofuscinosis, neuronal 8 (epilepsy, progressive with mental retardation) (CLN8), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202936L4 | Lenti ORF clone of Human ceroid-lipofuscinosis, neuronal 8 (epilepsy, progressive with mental retardation) (CLN8), mGFP tagged |
CNY 5,890.00 |
|
| RG202936 | CLN8 (tGFP-tagged) - Human ceroid-lipofuscinosis, neuronal 8 (epilepsy, progressive with mental retardation) (CLN8) |
CNY 4,000.00 |
|
| SC113338 | CLN8 (untagged)-Human ceroid-lipofuscinosis, neuronal 8 (epilepsy, progressive with mental retardation) (CLN8) |
CNY 2,400.00 |
|
| SC320766 | CLN8 (untagged)-Human ceroid-lipofuscinosis, neuronal 8 (epilepsy, progressive with mental retardation) (CLN8) |
CNY 2,400.00 |
