Papillary renal cell carcinoma (PRCC) (NM_005973) Human Tagged ORF Clone

CAT#: RC203805

PRCC (Myc-DDK-tagged)-Human papillary renal cell carcinoma (translocation-associated) (PRCC)

ORF Plasmid: DDK tGFP



  "NM_005973" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal PRCC Antibody
    • 100 ug

CNY 5,302.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms RCCP1; TPRC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203805 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCTGGTTGCTTACGCCAGCAGCGATGAGAGCGAGCCGGATGAGGCTGAGCCCGAGCCGGAGGAAG
AGGAGGCGGTGGCTCCTACATCTGGGCCCGCTTTAGGGGGCTTGTTCGCTTCTCTCCCTGCGCCCAAGGG
TCCGGCCTTGCTGCCTCCGCCCCCTCAGATGCTGGCGCCAGCCTTTCCCCCGCCGCTGTTGCTTCCCCCA
CCCACCGGAGACCCCAGGCTTCAGCCTCCTCCCCCCTTGCCCTTCGGCCTGGGAGGCTTCCCCCCACCTC
CAGGCGTGAGCCCGGCTGAAGCGGCGGGAGTTGGGGAGGGACTGGGATTGGGGTTGCCCTCGCCCCGAGG
CCCTGGCCTCAATCTGCCCCCTCCAATTGGCGGTGCCGGTCCCCCGCTGGGGCTTCCCAAGCCAAAGAAG
AGGAAAGAGCCCGTGAAGATCGCGGCGCCGGAGTTGCATAAGGGAGATTCAGATTCTGAGGAAGATGAAC
CCACAAAGAAGAAAACTATCCTTCAGGGATCCAGTGAGGGGACTGGTTTGTCTGCCTTGCTTCCCCAACC
TAAAAACCTGACTGTGAAAGAGACTAACAGGTTGCTCCTGCCCCATGCCTTCTCCCGCAAACCCTCGGAT
GGCTCCCCTGATACTAAGCCCTCCAGACTGGCTTCTAAGACCAAGACTTCCTCTCTTGCCCCTGTTGTGG
GCACCACAACCACCACTCCGTCGCCCTCTGCTATCAAGGCTGCTGCCAAGAGTGCTGCCCTGCAGGTGAC
AAAGCAGATCACGCAGGAAGAAGACGACAGTGATGAGGAAGTAGCCCCCGAAAACTTTTTCTCCCTCCCT
GAAAAGGCTGAGCCACCTGGAGTTGAGCCATACCCTTACCCCATCCCCACTGTCCCTGAAGAGCTGCCTC
CAGGCACGGAACCAGAGCCGGCTTTCCAGGACGATGCAGCCAATGCCCCCCTTGAATTCAAGATGGCAGC
AGGTTCAAGTGGGGCCCCTTGGATGCCTAAGCCTGGGGACGACTACAGCTACAATCAGTTTTCCACATAT
GGCGATGCCAATGCCGCTGGTGCTTATTATCAGGATTATTACAGTGGTGGCTACTATCCTGCACAGGACC
CGGCCCTGGTCCCCACCCAGGAAATTGCCCCAGATGCCTCCTTCATCGATGACGAAGCATTTAAGCGGCT
GCAGGGCAAGAGGAACCGAGGGAGAGAAGAAATCAACTTTGTGGAGATCAAAGGTGATGACCAGCTCAGT
GGGGCCCAGCAATGGATGACTAAGTCATTGACAGAAGAGAAAACCATGAAGTCATTCAGCAAAAAGAAAG
GTGAGCAGCCAACAGGCCAGCAGCGGCGGAAACACCAGATCACATATCTTATTCATCAGGCCAAGGAGCG
GGAGCTGGAACTGAAGAACACCTGGTCAGAGAACAAGCTCAGCCGCCGTCAGACCCAAGCCAAATATGGA
TTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203805 protein sequence
Red=Cloning site Green=Tags(s)

MSLVAYASSDESEPDEAEPEPEEEEAVAPTSGPALGGLFASLPAPKGPALLPPPPQMLAPAFPPPLLLPP
PTGDPRLQPPPPLPFGLGGFPPPPGVSPAEAAGVGEGLGLGLPSPRGPGLNLPPPIGGAGPPLGLPKPKK
RKEPVKIAAPELHKGDSDSEEDEPTKKKTILQGSSEGTGLSALLPQPKNLTVKETNRLLLPHAFSRKPSD
GSPDTKPSRLASKTKTSSLAPVVGTTTTTPSPSAIKAAAKSAALQVTKQITQEEDDSDEEVAPENFFSLP
EKAEPPGVEPYPYPIPTVPEELPPGTEPEPAFQDDAANAPLEFKMAAGSSGAPWMPKPGDDYSYNQFSTY
GDANAAGAYYQDYYSGGYYPAQDPALVPTQEIAPDASFIDDEAFKRLQGKRNRGREEINFVEIKGDDQLS
GAQQWMTKSLTEEKTMKSFSKKKGEQPTGQQRRKHQITYLIHQAKERELELKNTWSENKLSRRQTQAKYG
F

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005973
ORF Size 1473 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005973.3
RefSeq Size 2142 bp
RefSeq ORF 1476 bp
Locus ID 5546
UniProt ID Q92733
Protein Families Druggable Genome
MW 52.4 kDa
Gene Summary This gene encodes a protein that may play a role in pre-mRNA splicing. Chromosomal translocations (X;1)(p11;q21) that result in fusion of this gene to TFE3 (GeneID 7030) have been associated with papillary renal cell carcinoma. A PRCC-TFE3 fusion protein is expressed in affected carcinomas and is likely associated with altered gene transactivation. This fusion protein has also been associated with disruption of the cell cycle.[provided by RefSeq, Aug 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

其它Papillary renal cell carcinoma产品

Customer Reviews 
Loading...