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HNF1 beta (HNF1B) (NM_000458) Human Tagged ORF Clone
CAT＃: RC204453
HNF1B (Myc-DDK-tagged)-Human HNF1 homeobox B (HNF1B), transcript variant 1
ORF Plasmid: tGFP
"NM_000458" in other vectors (6)
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CNY 4,152.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADTKD3; FJHN; HNF-1-beta; HNF-1B; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; RCAD; T2D; TCF-2; TCF2; VHNF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204453 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCCAAGCTCACGTCGCTCCAGCAAGAACTCCTGAGCGCCCTGCTGAGCTCCGGGGTCACCAAGG AGGTGCTGGTTCAGGCCTTGGAGGAGTTGCTGCCATCCCCGAACTTCGGGGTGAAGCTGGAGACGCTGCC CCTGTCCCCTGGCAGCGGGGCCGAGCCCGACACCAAGCCGGTCTTCCATACTCTCACCAACGGCCACGCC AAGGGCCGCTTGTCCGGCGACGAGGGCTCCGAGGACGGCGACGACTATGACACACCTCCCATCCTCAAGG AGCTGCAGGCGCTCAACACCGAGGAGGCGGCGGAGCAGCGGGCGGAGGTGGACCGGATGCTCAGTGAGGA CCCTTGGAGGGCTGCTAAAATGATCAAGGGTTACATGCAGCAACACAACATCCCCCAGAGGGAGGTGGTC GATGTCACCGGCCTGAACCAGTCGCACCTCTCCCAGCATCTCAACAAGGGCACCCCTATGAAGACCCAGA AGCGTGCCGCTCTGTACACCTGGTACGTCAGAAAGCAACGAGAGATCCTCCGACAATTCAACCAGACAGT CCAGAGTTCTGGAAATATGACAGACAAAAGCAGTCAGGATCAGCTGCTGTTTCTCTTTCCAGAGTTCAGT CAACAGAGCCATGGGCCTGGGCAGTCCGATGATGCCTGCTCTGAGCCCACCAACAAGAAGATGCGCCGCA ACCGGTTCAAATGGGGGCCCGCGTCCCAGCAAATCTTGTACCAGGCCTACGATCGGCAAAAGAACCCCAG CAAGGAAGAGAGAGAGGCCTTAGTGGAGGAATGCAACAGGGCAGAATGTTTGCAGCGAGGGGTGTCCCCC TCCAAAGCCCACGGCCTGGGCTCCAACTTGGTCACTGAGGTCCGTGTCTACAACTGGTTTGCAAACCGCA GGAAGGAGGAGGCATTCCGGCAAAAGCTGGCCATGGACGCCTATAGCTCCAACCAGACTCACAGCCTGAA CCCTCTGCTCTCCCACGGCTCCCCCCACCACCAGCCCAGCTCCTCTCCTCCAAACAAGCTGTCAGGAGTG CGCTACAGCCAGCAGGGAAACAATGAGATCACTTCCTCCTCAACAATCAGTCACCATGGCAACAGCGCCA TGGTGACCAGCCAGTCGGTTTTACAGCAAGTCTCCCCAGCCAGCCTGGACCCAGGCCACAATCTCCTCTC ACCTGATGGTAAAATGATCTCAGTCTCAGGAGGAGGTTTGCCCCCAGTCAGCACCTTGACGAATATCCAC AGCCTCTCCCACCATAATCCCCAGCAATCTCAAAACCTCATCATGACACCCCTCTCTGGAGTCATGGCAA TTGCACAAAGCCTCAACACCTCCCAAGCACAGAGTGTCCCTGTCATCAACAGTGTGGCCGGCAGCCTGGC AGCCCTGCAGCCCGTCCAGTTCTCCCAGCAGCTGCACAGCCCTCACCAGCAGCCCCTCATGCAGCAGAGC CCAGGCAGCCACATGGCCCAGCAGCCCTTCATGGCAGCTGTGACTCAGCTGCAGAACTCACACATGTACG CACACAAGCAGGAACCCCCCCAGTATTCCCACACCTCCCGGTTTCCATCTGCAATGGTGGTCACAGATAC CAGCAGCATCAGTACACTCACCAACATGTCTTCAAGTAAACAGTGTCCTCTACAAGCCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204453 protein sequence
Red=Cloning site Green=Tags(s) MVSKLTSLQQELLSALLSSGVTKEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHA KGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVV DVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFS QQSHGPGQSDDACSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQRGVSP SKAHGLGSNLVTEVRVYNWFANRRKEEAFRQKLAMDAYSSNQTHSLNPLLSHGSPHHQPSSSPPNKLSGV RYSQQGNNEITSSSTISHHGNSAMVTSQSVLQQVSPASLDPGHNLLSPDGKMISVSGGGLPPVSTLTNIH SLSHHNPQQSQNLIMTPLSGVMAIAQSLNTSQAQSVPVINSVAGSLAALQPVQFSQQLHSPHQQPLMQQS PGSHMAQQPFMAAVTQLQNSHMYAHKQEPPQYSHTSRFPSAMVVTDTSSISTLTNMSSSKQCPLQAW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000458 |
| ORF Size | 1671 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000458.4 |
| RefSeq Size | 2842 bp |
| RefSeq ORF | 1674 bp |
| Locus ID | 6928 |
| UniProt ID | P35680 |
| Protein Families | Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors |
| Protein Pathways | Maturity onset diabetes of the young |
| MW | 61.3 kDa |
| Gene Summary | This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204453L1 | Lenti ORF clone of Human HNF1 homeobox B (HNF1B), transcript variant 1, Myc-DDK-tagged |
CNY 6,552.00 |
|
| RC204453L2 | Lenti ORF clone of Human HNF1 homeobox B (HNF1B), transcript variant 1, mGFP tagged |
CNY 6,552.00 |
|
| RC204453L3 | Lenti ORF clone of Human HNF1 homeobox B (HNF1B), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC204453L4 | Lenti ORF clone of Human HNF1 homeobox B (HNF1B), transcript variant 1, mGFP tagged |
CNY 6,552.00 |
|
| RG204453 | HNF1B (tGFP-tagged) - Human HNF1 homeobox B (HNF1B), transcript variant 1 |
CNY 5,752.00 |
|
| SC122562 | HNF1B (untagged)-Human HNF1 homeobox B (HNF1B), transcript variant 1 |
CNY 4,152.00 |
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