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TTLL4 (NM_014640) Human Tagged ORF Clone
CAT＃: RC205206
TTLL4 (Myc-DDK-tagged)-Human tubulin tyrosine ligase-like family, member 4 (TTLL4)
ORF Plasmid: tGFP
"NM_014640" in other vectors (6)
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CNY 12,520.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205206 representing NM_014640
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAGCAGGAACACAGCACTATAGTATTGGCCTCCGCCAGAAAAGCAGCTTCAAGCAGAGTGGTC CCTCAGGCACAGTACCTGCCACGCCACCTGAGAAACCCTCGGAGGGCAGAGTCTGGCCTCAGGCCCATCA GCAAGTGAAGCCAATCTGGAAGCTGGAAAAGAAGCAAGTGGAGACACTGTCAGCAGGGTTGGGCCCAGGC CTCTTGGGCGTCCCACCCCAGCCAGCATATTTCTTTTGCCCCAGCACTTTATGTAGCTCTGGGACCACGG CTGTCATTGCAGGCCACAGCAGTTCCTGTTACCTACACTCTCTCCCGGACTTGTTCAACAGCACCCTGCT ATACCGCCGCTCCAGCTATAGGCAAAAACCGTACCAGCAACTGGAGTCTTTCTGCTTGCGTTCGAGCCCG TCAGAAAAAAGCCCTTTTTCTCTCCCTCAAAAGAGCCTCCCTGTCAGTCTCACTGCCAACAAGGCCACTT CTTCCATGGTCTTCTCCATGGCCCAGCCCATGGCCTCCTCATCCACAGAACCATACCTCTGCTTGGCAGC GGCTGGGGAAAACCCTTCAGGGAAGAGCCTGGCCTCTGCCATCTCAGGGAAGATCCCATCTCCACTCTCT TCCTCCTATAAGCCCATGCTGAATAATAATTCCTTCATGTGGCCAAATAGCACGCCAGTGCCTTTATTGC AGACCACACAGGGCCTGAAGCCAGTATCGCCACCCAAGATCCAGCCTGTCTCCTGGCATCATTCAGGGGG TACTGGAGACTGTGCACCGCAGCCTGTTGACCATAAGGTGCCCAAAAGCATTGGCACTGTCCCAGCTGAT GCCAGTGCCCATATCGCCTTGTCTACCGCTAGCTCCCACGACACATCCACCACCAGTGTTGCCTCTTCCT GGTATAACCGGAATAACTTAGCCATGAGGGCAGAGCCACTTTCCTGTGCTCTGGATGACAGCTCTGATTC CCAGGATCCAACTAAGGAGATTCGGTTCACTGAGGCCGTGAGGAAATTGACCGCAAGAGGCTTTGAGAAG ATGCCGAGGCAAGGCTGCCAGCTTGAACAGTCTAGTTTCCTGAACCCCAGCTTCCAGTGGAATGTCCTCA ACAGGAGCAGGCGGTGGAAACCTCCTGCGGTAAATCAGCAGTTTCCTCAGGAGGATGCTGGATCGGTCAG GCGGGTCCTCCCTGGTGCCTCAGATACCTTGGGGTTGGACAATACAGTCTTCTGTACCAAGCGTATCAGC ATTCACCTCCTTGCCTCACATGCCAGTGGGCTCAATCACAACCCTGCCTGTGAATCTGTAATTGACTCCT CAGCATTTGGAGAAGGCAAAGCTCCAGGTCCCCCTTTTCCTCAAACTCTTGGCATAGCCAACGTGGCCAC CCGCCTCTCTTCCATCCAGCTGGGCCAGTCTGAGAAGGAGAGACCTGAGGAGGCCAGGGAGCTGGACTCA TCTGATAGGGATATTAGTTCAGCTACTGACCTCCAGCCAGATCAGGCTGAGACTGAAGATACAGAAGAAG AACTAGTAGATAGTTTGGAAGACTGTTGTGGCCGTGATGAGAATGAAGAGGAGGAGGGAGACTCAGAGTG CTCCTCATTAAGTGCTGTCTCCCCCAGCGAATCGGTGGCCATGATCTCTAGAAGCTGTATGGAAATTCTG ACCAAACCCCTTTCCAATCATGAGAAAGTTGTCCGACCAGCCCTCATCTACAGTCTCTTTCCCAACGTTC CCCCTACCATCTATTTTGGCACTCGGGATGAGAGAGTGGAGAAACTTCCCTGGGAACAGAGGAAGTTGCT CCGATGGAAGATGAGCACAGTGACCCCCAACATTGTCAAGCAGACCATTGGACGGTCCCACTTCAAAATC AGCAAAAGAAACGATGACTGGCTGGGCTGCTGGGGTCACCACATGAAGTCTCCTAGTTTCCGATCCATTC GAGAGCATCAGAAGCTAAACCATTTCCCAGGCTCATTCCAGATTGGGAGGAAGGACCGGCTATGGCGGAA CCTGTCACGTATGCAGAGCCGCTTTGGCAAGAAGGAGTTCAGTTTCTTCCCCCAGTCCTTTATCCTGCCC CAGGACGCCAAGCTCCTGCGCAAAGCGTGGGAGAGCAGCAGCCGCCAAAAGTGGATTGTGAAGCCACCAG CATCAGCTCGAGGCATTGGCATCCAGGTTATTCACAAGTGGAGTCAGCTCCCCAAGCGAAGGCCCCTCCT GGTACAGAGGTATCTACACAAACCCTACCTCATCAGCGGCAGCAAGTTTGACCTGCGGATCTATGTTTAT GTCACTTCCTACGATCCTCTGCGGATTTACCTCTTTTCAGATGGACTGGTCCGCTTTGCCAGTTGCAAGT ATTCGCCTTCCATGAAGAGCCTTGGCAATAAGTTCATGCACCTGACCAACTACAGTGTCAATAAAAAGAA TGCCGAGTACCAGGCCAATGCAGATGAAATGGCTTGCCAGGGCCACAAATGGGCACTGAAGGCTTTGTGG AACTACCTGAGCCAGAAGGGAGTCAATAGCGACTCCATCTGGGAGAAGATAAAGGATGTTGTTGTCAAAA CTATCATCTCGTCAGAGCCCTATGTGACCAGCCTGCTCAAGATGTATGTGCGACGGCCCTATAGCTGCCA TGAACTCTTTGGTTTTGACATCATGCTAGACGAAAACCTCAAGCCCTGGGTCCTGGAAGTCAACATTTCC CCAAGCCTCCACTCCAGCTCTCCACTGGATATCAGCATCAAAGGCCAGATGATTCGTGACCTTCTGAATC TGGCAGGTTTTGTCCTGCCCAATGCAGAGGATATCATTTCCAGCCCCAGCAGCTGCAGCAGCTCCACCAC CAGCCTGCCCACCTCCCCTGGGGACAAATGTCGAATGGCTCCAGAGCATGTCACTGCACAGAAGATGAAG AAAGCCTATTATCTGACCCAGAAAATTCCTGATCAGGACTTCTATGCATCTGTGCTGGATGTCCTGACAC CAGATGATGTTCGGATTCTGGTTGAGATGGAAGATGAGTTTTCTCGCCGTGGTCAGTTTGAACGAATTTT TCCTTCTCATATCTCCTCTCGCTATCTCCGCTTTTTTGAGCAGCCACGATATTTCAACATTCTCACCACC CAATGGGAACAGAAATACCATGGCAACAAGCTTAAAGGAGTAGATCTGCTCCGGAGTTGGTGCTACAAAG GGTTCCACATGGGAGTTGTCTCTGATTCTGCTCCAGTGTGGTCTCTCCCGACATCACTTCTGACTATCTC AAAGGATGACGTGATACTCAATGCCTTCAGCAAATCAGAGACTAGCAAGCTGGGAAAACAAAGCTCCTGT GAGGTTAGCCTACTACTCTCTGAAGACGGGACCACGCCCAAATCCAAGAAGACTCAAGCTGGCCTCTCCC CTTATCCCCAGAAACCCAGTTCCTCAAAGGACAGTGAGGACACCAGCAAAGAGCCCAGCCTTTCTACCCA GACGTTACCTGTGATCAAGTGCTCTGGGCAGACTTCAAGACTTTCTGCTTCCTCCACTTTCCAGTCAATC AGTGACTCCCTCCTGGCTGTGAGCCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205206 representing NM_014640
Red=Cloning site Green=Tags(s) MASAGTQHYSIGLRQKSSFKQSGPSGTVPATPPEKPSEGRVWPQAHQQVKPIWKLEKKQVETLSAGLGPG LLGVPPQPAYFFCPSTLCSSGTTAVIAGHSSSCYLHSLPDLFNSTLLYRRSSYRQKPYQQLESFCLRSSP SEKSPFSLPQKSLPVSLTANKATSSMVFSMAQPMASSSTEPYLCLAAAGENPSGKSLASAISGKIPSPLS SSYKPMLNNNSFMWPNSTPVPLLQTTQGLKPVSPPKIQPVSWHHSGGTGDCAPQPVDHKVPKSIGTVPAD ASAHIALSTASSHDTSTTSVASSWYNRNNLAMRAEPLSCALDDSSDSQDPTKEIRFTEAVRKLTARGFEK MPRQGCQLEQSSFLNPSFQWNVLNRSRRWKPPAVNQQFPQEDAGSVRRVLPGASDTLGLDNTVFCTKRIS IHLLASHASGLNHNPACESVIDSSAFGEGKAPGPPFPQTLGIANVATRLSSIQLGQSEKERPEEARELDS SDRDISSATDLQPDQAETEDTEEELVDSLEDCCGRDENEEEEGDSECSSLSAVSPSESVAMISRSCMEIL TKPLSNHEKVVRPALIYSLFPNVPPTIYFGTRDERVEKLPWEQRKLLRWKMSTVTPNIVKQTIGRSHFKI SKRNDDWLGCWGHHMKSPSFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILP QDAKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVY VTSYDPLRIYLFSDGLVRFASCKYSPSMKSLGNKFMHLTNYSVNKKNAEYQANADEMACQGHKWALKALW NYLSQKGVNSDSIWEKIKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFGFDIMLDENLKPWVLEVNIS PSLHSSSPLDISIKGQMIRDLLNLAGFVLPNAEDIISSPSSCSSSTTSLPTSPGDKCRMAPEHVTAQKMK KAYYLTQKIPDQDFYASVLDVLTPDDVRILVEMEDEFSRRGQFERIFPSHISSRYLRFFEQPRYFNILTT QWEQKYHGNKLKGVDLLRSWCYKGFHMGVVSDSAPVWSLPTSLLTISKDDVILNAFSKSETSKLGKQSSC EVSLLLSEDGTTPKSKKTQAGLSPYPQKPSSSKDSEDTSKEPSLSTQTLPVIKCSGQTSRLSASSTFQSI SDSLLAVSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014640 |
| ORF Size | 3597 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014640.3 |
| RefSeq Size | 4208 bp |
| RefSeq ORF | 3600 bp |
| Locus ID | 9654 |
| UniProt ID | Q14679 |
| Domains | TTL |
| MW | 133.2 kDa |
| Gene Summary | Glutamylase which preferentially modifies beta-tubulin and non-tubulin proteins, such as NAP1L1, NAP1L4 and CGAS. Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step. Involved in formation of short side-chains. Mediates initiation of polyglutamylation of nucleosome assembly proteins NAP1L1 and NAP1L4. Also acts as a monoglutamylase: generates monoglutamylation of CGAS, leading to impair the nucleotidyltransferase activity of CGAS.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ER proteins decipher the tubulin code to regulate organelle distribution
,null,
Nature
,PubMed ID 34912111
[TTLL4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205206L1 | Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 4 (TTLL4), Myc-DDK-tagged |
CNY 14,920.00 |
|
| RC205206L2 | Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 4 (TTLL4), mGFP tagged |
CNY 12,160.00 |
|
| RC205206L3 | Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 4 (TTLL4), Myc-DDK-tagged |
CNY 12,160.00 |
|
| RC205206L4 | Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 4 (TTLL4), mGFP tagged |
CNY 12,160.00 |
|
| RG205206 | TTLL4 (tGFP-tagged) - Human tubulin tyrosine ligase-like family, member 4 (TTLL4) |
CNY 14,120.00 |
|
| SC114928 | TTLL4 (untagged)-Human tubulin tyrosine ligase-like family, member 4 (TTLL4) |
CNY 12,528.00 |
