KPNA4 (NM_002268) Human Tagged ORF Clone
CAT#: RC205448
KPNA4 (Myc-DDK-tagged)-Human karyopherin alpha 4 (importin alpha 3) (KPNA4)
ORF Plasmid: tGFP
"NM_002268" in other vectors (6)
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CNY 3,880.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | IPOA3; QIP1; SRP3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205448 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACAACGAGAAACTGGACAACCAACGGCTCAAGAATTTCAAGAACAAAGGCCGCGACTTGGAGA CTATGAGAAGACAACGAAATGAAGTTGTAGTTGAATTAAGGAAGAATAAAAGAGATGAACATCTCTTAAA GAGAAGGAATGTACCACATGAAGATATCTGTGAAGACTCTGATATAGATGGTGATTATAGAGTGCAAAAT ACCTCTCTAGAAGCTATTGTTCAAAATGCTTCAAGTGATAACCAAGGAATTCAATTAAGTGCAGTTCAAG CTGCTAGGAAGCTTTTGTCCAGTGATCGAAATCCACCAATTGATGACTTAATAAAATCTGGAATATTGCC CATTTTAGTCCATTGTCTTGAAAGAGATGACAATCCTTCTTTACAGTTTGAAGCTGCATGGGCTTTGACA AACATTGCATCTGGAACTTCTGAACAAACTCAAGCAGTAGTTCAGTCCAATGCTGTGCCACTTTTCCTGA GGCTTCTCCATTCACCCCATCAGAATGTCTGTGAGCAAGCAGTGTGGGCATTGGGAAATATCATAGGTGA TGGGCCCCAGTGTAGAGATTATGTCATAAGTCTTGGAGTTGTGAAACCTTTACTTTCCTTCATAAGTCCA TCTATTCCTATAACATTCTTAAGAAATGTTACTTGGGTTATGGTCAACTTATGTCGCCACAAAGACCCAC CACCACCAATGGAAACCATTCAGGAGATTCTTCCAGCCCTTTGTGTTTTAATTCATCACACAGATGTAAA TATACTGGTAGACACAGTCTGGGCCCTCTCTTACCTTACTGATGCTGGCAATGAACAAATACAGATGGTA ATAGACTCTGGAATAGTTCCTCATTTGGTTCCTCTGCTCAGCCACCAGGAAGTTAAAGTTCAGACTGCTG CACTTAGAGCTGTGGGCAACATTGTTACTGGAACTGATGAGCAAACACAAGTAGTTTTGAACTGTGATGC TCTTTCACACTTCCCAGCACTCCTGACACATCCCAAAGAGAAAATTAATAAAGAAGCAGTGTGGTTCCTC TCCAACATCACTGCAGGAAATCAGCAGCAGGTACAGGCAGTAATTGATGCCAATCTTGTACCAATGATAA TACACCTTTTGGATAAGGGGGATTTTGGCACTCAAAAAGAAGCTGCTTGGGCCATAAGTAACTTAACAAT TAGTGGAAGGAAAGATCAAGTGGCTTACCTTATCCAACAAAATGTTATCCCACCTTTTTGCAACTTGCTG ACTGTAAAAGATGCACAAGTTGTGCAAGTAGTACTCGATGGACTAAGTAATATATTAAAAATGGCTGAAG ATGAGGCAGAAACCATAGGCAATCTTATAGAAGAATGTGGAGGGCTGGAGAAAATTGAACAACTTCAAAA TCATGAAAATGAAGACATCTACAAATTGGCCTATGAGATCATTGATCAGTTCTTCTCTTCAGATGATATT GATGAAGACCCTAGCCTTGTTCCAGAGGCAATTCAAGGCGGAACATTTGGTTTCAATTCATCTGCCAATG TACCAACAGAAGGGTTCCAGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205448 protein sequence
Red=Cloning site Green=Tags(s) MADNEKLDNQRLKNFKNKGRDLETMRRQRNEVVVELRKNKRDEHLLKRRNVPHEDICEDSDIDGDYRVQN TSLEAIVQNASSDNQGIQLSAVQAARKLLSSDRNPPIDDLIKSGILPILVHCLERDDNPSLQFEAAWALT NIASGTSEQTQAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFISP SIPITFLRNVTWVMVNLCRHKDPPPPMETIQEILPALCVLIHHTDVNILVDTVWALSYLTDAGNEQIQMV IDSGIVPHLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKINKEAVWFL SNITAGNQQQVQAVIDANLVPMIIHLLDKGDFGTQKEAAWAISNLTISGRKDQVAYLIQQNVIPPFCNLL TVKDAQVVQVVLDGLSNILKMAEDEAETIGNLIEECGGLEKIEQLQNHENEDIYKLAYEIIDQFFSSDDI DEDPSLVPEAIQGGTFGFNSSANVPTEGFQF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002268 |
ORF Size | 1563 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002268.5 |
RefSeq Size | 8981 bp |
RefSeq ORF | 1566 bp |
Locus ID | 3840 |
UniProt ID | O00629 |
Domains | Armadillo_seg, IBB |
MW | 57.9 kDa |
Gene Summary | The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Long noncoding RNA NEAT1 drives aggressive endometrial cancer progression via miR-361-regulated networks involving STAT3 and tumor microenvironment-related genes
,Dong, P;Xiong, Y;Yue, J;Xu, D;Ihira, K;Konno, Y;Kobayashi, N;Todo, Y;Watari, H;,
J. Exp. Clin. Cancer Res.
,PubMed ID 31287002
[KPNA4]
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205448L1 | Lenti ORF clone of Human karyopherin alpha 4 (importin alpha 3) (KPNA4), Myc-DDK-tagged |
CNY 6,280.00 |
|
RC205448L2 | Lenti ORF clone of Human karyopherin alpha 4 (importin alpha 3) (KPNA4), mGFP tagged |
CNY 5,890.00 |
|
RC205448L3 | Lenti ORF clone of Human karyopherin alpha 4 (importin alpha 3) (KPNA4), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205448L4 | Lenti ORF clone of Human karyopherin alpha 4 (importin alpha 3) (KPNA4), mGFP tagged |
CNY 5,890.00 |
|
RG205448 | KPNA4 (tGFP-tagged) - Human karyopherin alpha 4 (importin alpha 3) (KPNA4) |
CNY 5,480.00 |
|
SC127533 | KPNA4 (untagged)-Human karyopherin alpha 4 (importin alpha 3) (KPNA4) |
CNY 3,888.00 |