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Fbx32 (FBXO32) (NM_148177) Human Tagged ORF Clone
CAT＃: RC206217
FBXO32 (Myc-DDK-tagged)-Human F-box protein 32 (FBXO32), transcript variant 2
ORF Plasmid: tGFP
"NM_148177" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fbx32; MAFbx |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206217 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATATTTTGGAAAAAGTGGTACTGAAAGTCCTTGAAGACCAGCAAAACATTAGACTAATAAGGGAAC TACTCCAGACCCTCTACACATCCTTATGTACACTGGTCCAAAGAGTCGGCAAGTCTGTGCTGGTCGGGAA CATTAACATGTGGGTGTATCGGATGGAGACGATTCTCCACTGGCAGCAGCAGCTGAACAACATTCAGATC ACCAGGCCTGCCTTCAAAGGCCTCACCTTCACTGACCTGCCTTTGTGCCTACAACTGAACATCATGCAGA GGCTGAGCGACGGGCGGGACCTGGTCAGCCTGGGCCAGGCTGCCCCCGACCTGCACGTGCTCAGCGAAGA CCGGCTGCTGTGGAAGAAACTCTGCCAGTACCACTTCTCCGAGCGGCAGATCCGCAAACGATTAATTCTG TCAGACAAAGGGCAGCTGGATTGGAAGAAGATGTATTTCAAACTCGTCCGATGTTACCCAAGGAAAGAGC AGTATGGAGATACCCTTCAGCTCCGCAAACACTGTCACATCCTTTCCTGGAAGGGCACTGACCATCCGTG CACTGCCAATAACCCAGAGAGCTGCTCCGTTTCACTTTCACCCCAGGACTTTATCAACTTGTTCAAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206217 protein sequence
Red=Cloning site Green=Tags(s) MNILEKVVLKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVYRMETILHWQQQLNNIQI TRPAFKGLTFTDLPLCLQLNIMQRLSDGRDLVSLGQAAPDLHVLSEDRLLWKKLCQYHFSERQIRKRLIL SDKGQLDWKKMYFKLVRCYPRKEQYGDTLQLRKHCHILSWKGTDHPCTANNPESCSVSLSPQDFINLFKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_148177 |
| ORF Size | 630 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_148177.2 |
| RefSeq Size | 6435 bp |
| RefSeq ORF | 633 bp |
| Locus ID | 114907 |
| Domains | F-box |
| MW | 24.8 kDa |
| Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206217L1 | Lenti ORF clone of Human F-box protein 32 (FBXO32), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC206217L2 | Lenti ORF clone of Human F-box protein 32 (FBXO32), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC206217L3 | Lenti ORF clone of Human F-box protein 32 (FBXO32), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206217L4 | Lenti ORF clone of Human F-box protein 32 (FBXO32), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG206217 | FBXO32 (tGFP-tagged) - Human F-box protein 32 (FBXO32), transcript variant 2 |
CNY 4,000.00 |
|
| SC101194 | FBXO32 (untagged)-Human F-box protein 32 (FBXO32), transcript variant 2 |
CNY 2,400.00 |
