CDCA4 (NM_017955) Human Tagged ORF Clone
CAT#: RC206833
CDCA4 (Myc-DDK-tagged)-Human cell division cycle associated 4 (CDCA4), transcript variant 1
ORF Plasmid: tGFP
"NM_017955" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HEPP; SEI-3/HEPP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206833 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGCACGAGGACTGAAGAGGAAATGTGTTGGCCACGAGGAAGACGTGGAGGGAGCCCTGGCCGGCT TGAAGACAGTGTCCTCATACAGCCTGCAGCGGCAGTCGCTCCTGGACATGTCTCTGGTGAAGTTGCAGCT TTGCCACATGCTTGTGGAGCCCAATCTGTGCCGCTCAGTCCTCATTGCCAACACGGTCCGGCAGATCCAA GAGGAGATGACGCAGGATGGGACGTGGCGCACAGTGGCACCCCAGGCTGCAGAGCGGGCGCCGCTCAACC GCTTGGTCTCCACGGAGATCCTGTGCCGTGCAGCGTGGGGGCAAGAGGGGGCACATCCTGCTCCTGGCTT GGGGGACGGCCACACACAGGGTCCAGTTTCTGACCTTTGCCCAGTCACCTCAGCACAGGCACCAAGGCAC CTGCAGAGCAGCGCCTGGGAGATGGATGGCCCTCGAGAAAACAGAGGAAGCTTTCACAAGTCACTTGATC AGATATTTGAAACGCTGGAGACTAAAAACCCCAGCTGCATGGAAGAGCTGTTCTCAGACGTGGACAGCCC CTACTACGACCTGGACACAGTACTGACAGGCATGATGGGGGGTGCCAGGCCGGGCCCCTGCGAAGGGCTC GAGGGCTTGGCTCCGGCCACCCCAGGCCCTAGCTCCAGCTGCAAGTCCGACCTGGGCGAGCTGGACCACG TGGTGGAGATCCTGGTGGAGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206833 protein sequence
Red=Cloning site Green=Tags(s) MFARGLKRKCVGHEEDVEGALAGLKTVSSYSLQRQSLLDMSLVKLQLCHMLVEPNLCRSVLIANTVRQIQ EEMTQDGTWRTVAPQAAERAPLNRLVSTEILCRAAWGQEGAHPAPGLGDGHTQGPVSDLCPVTSAQAPRH LQSSAWEMDGPRENRGSFHKSLDQIFETLETKNPSCMEELFSDVDSPYYDLDTVLTGMMGGARPGPCEGL EGLAPATPGPSSSCKSDLGELDHVVEILVET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_017955 |
ORF Size | 723 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017955.4 |
RefSeq Size | 2453 bp |
RefSeq ORF | 726 bp |
Locus ID | 55038 |
UniProt ID | Q9BXL8 |
Protein Families | ES Cell Differentiation/IPS |
MW | 26.1 kDa |
Gene Summary | This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1. [provided by RefSeq, May 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206833L3 | Lenti ORF clone of Human cell division cycle associated 4 (CDCA4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206833L4 | Lenti ORF clone of Human cell division cycle associated 4 (CDCA4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG206833 | CDCA4 (tGFP-tagged) - Human cell division cycle associated 4 (CDCA4), transcript variant 1 |
CNY 4,000.00 |
|
SC321232 | CDCA4 (untagged)-Human cell division cycle associated 4 (CDCA4), transcript variant 1 |
CNY 2,400.00 |