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NXF1 (NM_006362) Human Tagged ORF Clone
CAT＃: RC207039
NXF1 (Myc-DDK-tagged)-Human nuclear RNA export factor 1 (NXF1), transcript variant 1
ORF Plasmid: tGFP
"NM_006362" in other vectors (4)
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CNY 4,608.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MEX67; TAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207039 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAGGGGAAGTCGTACAGCGAACACGATGATGAACGCGTTAATTTCCCTCAAAGAAAGAAGA AAGGCCGGGGTCCCTTCCGGTGGAAATATGGTGAAGGAAACCGTAGGTCTGGAAGAGGCGGTTCTGGTAT TCGGTCTTCCCGCCTTGAGGAAGATGATGGAGATGTGGCAATGAGTGATGCCCAGGATGGTCCCCGAGTA CGATACAACCCCTATACCACCCGACCTAACCGTCGGGGTGATACTTGGCATGATCGAGATCGCATTCATG TTACTGTGCGGAGAGACAGAGCTCCTCCAGAGAGAGGAGGGGCTGGCACCAGCCAGGATGGGACCTCAAA GAACTGGTTCAAGATTACAATTCCTTATGGCAGAAAGTATGACAAGGCATGGCTCCTGAGCATGATTCAG AGCAAGTGCAGTGTGCCCTTCACCCCTATTGAGTTTCACTATGAGAATACACGGGCCCAGTTCTTCGTTG AAGACGCCAGTACTGCCTCTGCATTGAAGGCTGTCAACTATAAGATTTTGGATCGGGAGAACCGAAGGAT ATCTATCATCATCAACTCTTCTGCTCCACCCCACACTATACTGAATGAACTGAAGCCAGAACAAGTAGAA CAGCTAAAGCTGATCATGAGCAAACGATACGATGGCTCCCAACAAGCCCTTGACCTCAAAGGCCTCCGTT CAGACCCAGATTTGGTGGCCCAGAACATTGACGTTGTCCTGAATCGCAGAAGCTGTATGGCAGCTACCCT GAGGATCATTGAAGAGAACATCCCTGAGCTATTGTCCTTGAACTTGAGCAACAACAGGCTGTACAGGCTG GATGACATGTCTAGCATTGTTCAGAAGGCACCCAACCTGAAGATCCTAAACCTTTCTGGAAATGAATTGA AGTCTGAGCGGGAATTGGACAAGATAAAGGGGCTGAAGCTAGAAGAGCTCTGGCTCGATGGAAACTCCCT GTGTGACACCTTCCGAGACCAGTCCACCTACATCAGCGCCATTCGCGAACGATTTCCCAAGTTACTACGC CTGGATGGCCATGAGCTACCCCCACCAATTGCCTTTGATGTTGAAGCCCCCACGACGTTACCGCCCTGCA AGGGAAGCTATTTTGGAACAGAAAACTTGAAGAGTCTGGTCTTGCACTTCCTGCAACAGTACTATGCAAT TTACGACTCTGGAGACCGACAAGGGCTCCTGGATGCCTACCATGATGGGGCCTGCTGTTCCCTGAGCATT CCTTTCATTCCTCAGAACCCTGCCCGAAGCAGCTTAGCCGAGTATTTCAAGGATAGCAGAAATGTGAAGA AGCTTAAAGACCCTACCTTGCGGTTCCGGCTGCTGAAGCACACGCGTCTCAACGTTGTTGCCTTCCTCAA TGAGTTGCCCAAAACCCAGCACGACGTCAATTCCTTCGTGGTAGACATAAGCGCCCAGACAAGCACATTG CTGTGTTTTTCTGTCAATGGAGTCTTCAAGGAAGTGGACGGAAAGTCCCGGGATTCTTTGCGAGCCTTCA CCCGGACATTCATTGCTGTTCCTGCTAGCAATTCAGGGCTATGTATTGTAAATGATGAGCTATTTGTGCG GAATGCCAGTTCTGAAGAGATCCAAAGAGCCTTCGCTATGCCTGCACCCACGCCTTCCTCCAGCCCGGTG CCCACCCTCTCTCCAGAGCAGCAGGAAATGTTGCAAGCATTCTCTACCCAGTCTGGCATGAACCTCGAGT GGTCCCAGAAGTGCCTTCAGGACAACAACTGGGACTACACCAGATCTGCCCAGGCCTTCACTCATCTCAA GGCCAAGGGCGAGATCCCAGAAGTGGCATTCATGAAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC207039 protein sequence
Red=Cloning site Green=Tags(s) MADEGKSYSEHDDERVNFPQRKKKGRGPFRWKYGEGNRRSGRGGSGIRSSRLEEDDGDVAMSDAQDGPRV RYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQ SKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVE QLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRL DDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYISAIRERFPKLLR LDGHELPPPIAFDVEAPTTLPPCKGSYFGTENLKSLVLHFLQQYYAIYDSGDRQGLLDAYHDGACCSLSI PFIPQNPARSSLAEYFKDSRNVKKLKDPTLRFRLLKHTRLNVVAFLNELPKTQHDVNSFVVDISAQTSTL LCFSVNGVFKEVDGKSRDSLRAFTRTFIAVPASNSGLCIVNDELFVRNASSEEIQRAFAMPAPTPSSSPV PTLSPEQQEMLQAFSTQSGMNLEWSQKCLQDNNWDYTRSAQAFTHLKAKGEIPEVAFMK SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006362 |
| ORF Size | 1857 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006362.5 |
| RefSeq Size | 2346 bp |
| RefSeq ORF | 1860 bp |
| Locus ID | 10482 |
| UniProt ID | Q9UBU9 |
| Domains | LRR, NTF2, TAP_C |
| MW | 70.2 kDa |
| Gene Summary | This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207039L3 | Lenti ORF clone of Human nuclear RNA export factor 1 (NXF1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207039L4 | Lenti ORF clone of Human nuclear RNA export factor 1 (NXF1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG207039 | NXF1 (tGFP-tagged) - Human nuclear RNA export factor 1 (NXF1), transcript variant 1 |
CNY 6,208.00 |
|
| SC116148 | NXF1 (untagged)-Human nuclear RNA export factor 1 (NXF1), transcript variant 1 |
CNY 4,616.00 |
