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NXF1 (NM_006362) Human Untagged Clone
CAT＃: SC116148
NXF1 (untagged)-Human nuclear RNA export factor 1 (NXF1), transcript variant 1
CNY 4,616.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MEX67; TAP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116148 sequence for NM_006362 edited (data generated by NextGen Sequencing)
ATGGCGGACGAGGGGAAGTCGTACAGCGAACACGATGATGAACGCGTTAATTTCCCTCAA AGAAAGAAGAAAGGCCGGGGTCCCTTCCGGTGGAAATATGGTGAAGGAAACCGTAGGTCT GGAAGAGGCGGTTCTGGTATTCGGTCTTCCCGCCTTGAGGAAGATGATGGAGATGTGGCA ATGAGTGATGCCCAGGATGGTCCCCGAGTACGATACAACCCCTATACCACCCGACCTAAC CGTCGGGGTGATACTTGGCATGATCGAGATCGCATTCATGTTACTGTGCGGAGAGACAGA GCTCCTCCAGAGAGAGGAGGGGCTGGCACCAGCCAGGATGGGACCTCAAAGAACTGGTTC AAGATTACAATTCCTTATGGCAGAAAGTATGACAAGGCATGGCTCCTGAGCATGATTCAG AGCAAGTGCAGTGTGCCCTTCACCCCTATTGAGTTTCACTATGAGAATACACGGGCCCAG TTCTTCGTTGAAGACGCCAGTACTGCCTCTGCATTGAAGGCTGTCAACTATAAGATTTTG GATCGGGAGAACCGAAGGATATCTATCATCATCAACTCTTCTGCTCCACCCCACACTATA CTGAATGAACTGAAGCCAGAACAAGTAGAACAGCTAAAGCTGATCATGAGCAAACGATAC GATGGCTCCCAACAAGCCCTTGACCTCAAAGGCCTCCGTTCAGACCCAGATTTGGTGGCC CAGAACATTGACGTTGTCCTGAATCGCAGAAGCTGTATGGCAGCTACCCTGAGGATCATT GAAGAGAACATCCCTGAGCTATTGTCCTTGAACTTGAGCAACAACAGGCTGTACAGGCTG GATGACATGTCTAGCATTGTTCAGAAGGCACCCAACCTGAAGATCCTAAACCTTTCTGGA AATGAATTGAAGTCTGAGCGGGAATTGGACAAGATAAAGGGGCTGAAGCTAGAAGAGCTC TGGCTCGATGGAAACTCCCTGTGTGACACCTTCCGAGACCAGTCCACCTACATCAGCGCC ATTCGCGAACGATTTCCCAAGTTACTACGCCTGGATGGCCATGAGCTACCCCCACCAATT GCCTTTGATGTTGAAGCCCCCACGACGTTACCGCCCTGCAAGGGAAGCTATTTTGGAACA GAAAACTTGAAGAGTCTGGTCTTGCACTTCCTGCAACAGTACTATGCAATTTACGACTCT GGAGACCGACAAGGGCTCCTGGATGCCTACCATGATGGGGCCTGCTGTTCCCTGAGCATT CCTTTCATTCCTCAGAACCCTGCCCGAAGCAGCTTAGCCGAGTATTTCAAGGATAGCAGA AATGTGAAGAAGCTTAAAGACCCTACCTTGCGGTTCCGGCTGCTGAAGCACACGCGTCTC AACGTTGTTGCCTTCCTCAATGAGTTGCCCAAAACCCAGCACGACGTCAATTCCTTCGTG GTAGACATAAGCGCCCAGACAAGCACATTGCTGTGTTTTTCTGTCAATGGAGTCTTCAAG GAAGTGGACGGAAAGTCCCGGGATTCTTTGCGAGCCTTCACCCGGACATTCATTGCTGTT CCTGCTAGCAATTCAGGGCTATGTATTGTAAATGATGAGCTATTTGTGCGGAATGCCAGT TCTGAAGAGATCCAAAGAGCCTTCGCTATGCCTGCACCCACGCCTTCCTCCAGCCCGGTG CCCACCCTCTCTCCAGAGCAGCAGGAAATGTTGCAAGCATTCTCTACCCAGTCTGGCATG AACCTCGAGTGGTCCCAGAAGTGCCTTCAGGACAACAACTGGGACTACACCAGATCTGCC CAGGCCTTCACTCATCTCAAGGCCAAGGGCGAGATCCCAGAAGTGGCATTCATGAAGTGA Clone variation with respect to NM_006362.4 >OriGene 5' read for NM_006362 unedited
TTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCGGCGCGCGAC GTGCTTTGCTGTATAAATGCGGTGGCGCCCGGCGTAGGGACACTTCGGTCCTGAGCGCTT GGGAGTTAGGTTGTTTGCCGGCGTAGCGGCCAGCGCCTGAGCCCGCCCTTGATCTTCGCT GTGGCATGGCGGACGAGGGGAAGTCGTACAGCGAACACGATGATGAACGCGTTAATTTCC CTCAAAGAAAGAAGAAAGGCCGGGGTCCCTTCCGGTGGAAATATGGTGAAGGAAACCGTA GGTCTGGAAGAGGCGGTTCTGGTATTCGGTCTTCCCGCCTTGAGGAAGATGATGGAGATG TGGCAATGAGTGATGCCCAGGATGGTCCCCGAGTACGATACAACCCCTATACCACCCGAC CTAACCGTCGGGGTGATACTTGGCATGATCGAGATCGCATTCATGTTACTGTGCGGAGAG ACAGAGCTCCTCCAGAGAGAGGAGGGGCTGGCACCAGCCAGGATGGGACCTCAAAGAACT GGTTCAAGATTACAATTCCTTATGGCAGAAAGTATGACAAGGCATGGCTCCTGAGCATGA TTCAGAGCAAGTGCAGTGTGCCCTTCACCCCTATTGAGTTTCACTATGAGAATACACGGG CCCAGTTCTTCGTTGAAGACGCCAGTACTGCCTCTGCATTGAAGGCTGTCAACTATAAGA TTTTGGATCGGGAGAACCGANAGATATCTATCATCATCAACTCTTCTGCTCCACCCCCAC TATACTGNATGAAACTGAGCCAGNAACAGTAGAACAGCTAAAGCTGATCATGAGCAAACG ATACGATGGCTCCCAACAGCCCTTGACCTCAAGGNC >OriGene 3' read for NM_006362 unedited
GAACCGCGCCTTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACAACATCACTCTTTAT ATTAAAAAGTGCAGAACACGAAATACAACATCACTCTTTATATTAAAAAGTAAGGAGGTC CTGGGGTTAAGTACACAAAGCACCTAAGTCCTTCGGGTAGTTTAGTGTCAGTTCTACAAA GTAAGCTTTGGCTTCCTGGCACCAGTGAGGCTCAATCTTCTGAAGTCTTCCAGAAGGCAG GCGAGGAGAGGGATCAGGCAGCCCTCCCTCCCTCGGTCACAGTCACGGGGCGGCCTCGGG CCAGACAGGAGGAGATGACAGACGACAACCAGACGGTAATATCCAAGGACTATTTACAGG GGGGACTGCTTCTGAGGCATGACTACGATCACTTCATGAATGCCACTTCTGGGATCTCGC CCTTGGCCTTGAGATGAGTGAAGGCCTGGGCAGATCTGGTGTAGTCCCAGTTGTTGTCCT GAAGGCACTTTTGGGACCACTCGAGGTTCATGCCAGACTGGGTAGAGAATGCTTGCAACA TTTCCTGCTGCTCTGGAGAGAGGGTGGGCACCGGGCTGGAGGAAGGCGTGGGTGCACGCC TANCGAAGGCTCTTTGGATCTCTTAAGAACTGGCATTCCGCACAAATGGCTCATCATTTA CAATACATAGCCCCTGAATTGCTAGCAAGGACAACATTGAATGTCCGGGGGAAAGCTCCA AAATAACCCGGGAACTTTCCGCCCACTTTCCTTTAAAACTCCTTGGCCAGAAAAACACAN CAATGTGCTTGTCTTGGGCCTTTTGTCTCCCACAAAGAATTGCCCCCCGCTGGGCTCGGG CCCCCTCGTGGAAGCCCCCCTCGAACCCTGTGCTCACCACCGCACCGCAGGTAGGCTTAA ACTTTCTCATTTTTGTTTCTTAAAATAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006362 |
| Insert Size | 2640 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006362.3, NP_006353.2 |
| RefSeq Size | 2307 bp |
| RefSeq ORF | 1860 bp |
| Locus ID | 10482 |
| UniProt ID | Q9UBU9 |
| Domains | LRR, NTF2, TAP_C |
| Gene Summary | This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer isoform (1). |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207039 | NXF1 (Myc-DDK-tagged)-Human nuclear RNA export factor 1 (NXF1), transcript variant 1 |
CNY 4,608.00 |
|
| RC207039L3 | Lenti ORF clone of Human nuclear RNA export factor 1 (NXF1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207039L4 | Lenti ORF clone of Human nuclear RNA export factor 1 (NXF1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG207039 | NXF1 (tGFP-tagged) - Human nuclear RNA export factor 1 (NXF1), transcript variant 1 |
CNY 6,208.00 |
