PTP epsilon (PTPRE) (NM_006504) Human Tagged ORF Clone

CAT#: RC207950

PTPRE (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, E (PTPRE), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_006504" in other vectors (6)

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CNY 5,128.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PTPRE mouse monoclonal antibody, clone OTI4B2 (formerly 4B2)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HPTPE; PTPE; R-PTP-EPSILON
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207950 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCTTGTGTCCACTCCTGCTGGTGGGTTTTAGCTTGCCGCTCGCCAGGGCTCTCAGGGGCAACG
AGACCACTGCCGACAGCAACGAGACAACCACGACCTCAGGCCCTCCGGACCCGGGCGCCTCCCAGCCGCT
GCTGGCCTGGCTGCTACTGCCGCTGCTGCTCCTCCTCCTCGTGCTCCTTCTCGCCGCCTACTTCTTCAGG
TTCAGGAAGCAGAGGAAAGCTGTGGTCAGCACCAGCGACAAGAAGATGCCCAACGGAATCTTGGAGGAGC
AAGAGCAGCAAAGGGTGATGCTGCTCAGCAGGTCACCCTCAGGGCCCAAGAAGTATTTTCCCATCCCCGT
GGAGCACCTGGAGGAGGAGATCCGTATCAGATCCGCCGACGACTGCAAGCAGTTTCGGGAGGAGTTCAAC
TCATTGCCATCTGGACACATACAAGGAACTTTTGAACTGGCAAATAAAGAAGAAAACAGAGAAAAAAACA
GATATCCCAACATCCTTCCCAATGACCATTCTAGGGTGATTCTGAGCCAACTGGATGGAATTCCCTGTTC
AGACTACATCAATGCTTCCTACATAGATGGTTACAAAGAGAAGAATAAATTCATAGCAGCTCAAGGTCCC
AAACAGGAAACGGTTAACGACTTCTGGAGAATGGTCTGGGAGCAAAAGTCTGCGACCATCGTCATGTTAA
CAAACTTGAAAGAAAGGAAAGAGGAAAAGTGCCATCAGTACTGGCCCGACCAAGGCTGCTGGACCTATGG
AAACATCCGGGTGTGCGTGGAGGACTGCGTGGTTTTGGTCGACTACACCATCCGGAAGTTCTGCATACAG
CCACAGCTCCCCGACGGCTGCAAAGCCCCCAGGCTGGTCTCACAGCTGCACTTCACCAGCTGGCCCGACT
TCGGAGTGCCTTTTACCCCCATTGGGATGCTGAAGTTCCTCAAGAAAGTAAAGACGCTCAACCCCGTGCA
CGCTGGGCCCATCGTGGTCCACTGTAGCGCGGGCGTGGGCCGGACGGGCACCTTCATTGTGATCGATGCC
ATGATGGCCATGATGCACGCGGAGCAGAAGGTGGATGTGTTTGAATTTGTGTCTCGAATCCGTAATCAGC
GCCCTCAGATGGTTCAAACGGATATGCAGTACACGTTCATCTACCAAGCCTTACTCGAGTACTACCTCTA
CGGGGACACAGAGCTGGACGTGTCCTCACTGGAGAAGCACCTGCAGACCATGCACGGCACCACAACCCAC
TTCGACAAGATCGGGCTGGAGGAGGAGTTCAGGAAATTGACAAATGTCCGGATCATGAAGGAGAACATGA
GGACGGGCAACTTGCCGGCAAACATGAAGAAGGCCAGGGTCATCCAGATCATCCCGTATGACTTCAACCG
AGTGATCCTTTCCATGAAAAGGGGTCAAGAATACACAGACTACATCAACGCATCCTTCATAGACGGCTAC
CGACAGAAGGACTATTTCATCGCCACCCAGGGGCCACTGGCACACACGGTTGAGGACTTCTGGAGGATGA
TCTGGGAATGGAAATCCCACACTATCGTGATGCTGACGGAGGTGCAGGAGAGAGAGCAGGATAAATGCTA
CCAGTATTGGCCAACCGAGGGCTCAGTTACTCATGGAGAAATAACGATTGAGATAAAGAATGATACCCTT
TCAGAAGCCATCAGTATACGAGACTTTCTGGTCACTCTCAATCAGCCCCAGGCCCGCCAGGAGGAGCAGG
TCCGAGTAGTGCGCCAGTTTCACTTCCACGGCTGGCCTGAGATCGGGATTCCCGCCGAGGGCAAAGGCAT
GATTGACCTCATCGCAGCCGTGCAGAAGCAGCAGCAGCAGACAGGCAACCACCCCATCACCGTGCACTGC
AGTGCCGGAGCTGGGCGAACAGGTACATTCATAGCCCTCAGCAACATTTTGGAGCGAGTAAAAGCCGAGG
GGCTTTTAGATGTATTTCAAGCTGTGAAGAGTTTACGACTTCAGAGACCACATATGGTGCAAACCCTGGA
ACAGTATGAATTCTGCTACAAAGTGGTACAAGATTTTATTGATATATTTTCTGATTATGCTAATTTCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207950 protein sequence
Red=Cloning site Green=Tags(s)

MEPLCPLLLVGFSLPLARALRGNETTADSNETTTTSGPPDPGASQPLLAWLLLPLLLLLLVLLLAAYFFR
FRKQRKAVVSTSDKKMPNGILEEQEQQRVMLLSRSPSGPKKYFPIPVEHLEEEIRIRSADDCKQFREEFN
SLPSGHIQGTFELANKEENREKNRYPNILPNDHSRVILSQLDGIPCSDYINASYIDGYKEKNKFIAAQGP
KQETVNDFWRMVWEQKSATIVMLTNLKERKEEKCHQYWPDQGCWTYGNIRVCVEDCVVLVDYTIRKFCIQ
PQLPDGCKAPRLVSQLHFTSWPDFGVPFTPIGMLKFLKKVKTLNPVHAGPIVVHCSAGVGRTGTFIVIDA
MMAMMHAEQKVDVFEFVSRIRNQRPQMVQTDMQYTFIYQALLEYYLYGDTELDVSSLEKHLQTMHGTTTH
FDKIGLEEEFRKLTNVRIMKENMRTGNLPANMKKARVIQIIPYDFNRVILSMKRGQEYTDYINASFIDGY
RQKDYFIATQGPLAHTVEDFWRMIWEWKSHTIVMLTEVQEREQDKCYQYWPTEGSVTHGEITIEIKNDTL
SEAISIRDFLVTLNQPQARQEEQVRVVRQFHFHGWPEIGIPAEGKGMIDLIAAVQKQQQQTGNHPITVHC
SAGAGRTGTFIALSNILERVKAEGLLDVFQAVKSLRLQRPHMVQTLEQYEFCYKVVQDFIDIFSDYANFK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006504
ORF Size 2100 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006504.6
RefSeq Size 5392 bp
RefSeq ORF 2103 bp
Locus ID 5791
UniProt ID P23469
Domains Y_phosphatase, PTPc_motif
Protein Families Druggable Genome, Phosphatase, Transmembrane
MW 80.6 kDa
Gene Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Several alternatively spliced transcript variants of this gene have been reported, at least two of which encode a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains; another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokine-induced SATA signaling, as well as the activation of voltage-gated K+ channels. [provided by RefSeq, Oct 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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