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MRGX1 (MRGPRX1) (NM_147199) Human Tagged Lenti ORF Clone
CAT＃: RC211487L1
- LentiORF®
Lenti-ORF clone of MRGPRX1 (Myc-DDK-tagged)-Human MAS-related GPR, member X1 (MRGPRX1)
"NM_147199" in other vectors (6)
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CNY 5,890.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPCR; MGRG2; MRGX1; SNSR4 |
| Vector | pLenti-C-Myc-DDK |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC211487).
|
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_147199 |
| ORF Size | 966 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_147199.3, NP_671732.3 |
| RefSeq Size | 1190 bp |
| RefSeq ORF | 969 bp |
| Locus ID | 259249 |
| UniProt ID | Q96LB2 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 36.3 kDa |
| Gene Summary | Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22)(PubMed:26582731). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine-induced itch, in a histamine-independent manner.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211487 | MRGPRX1 (Myc-DDK-tagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 3,600.00 |
|
| RC211487L2 | Lenti-ORF clone of MRGPRX1 (mGFP-tagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 5,890.00 |
|
| RC211487L3 | Lenti-ORF clone of MRGPRX1 (Myc-DDK-tagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 5,890.00 |
|
| RC211487L4 | Lenti-ORF clone of MRGPRX1 (mGFP-tagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 6,000.00 |
|
| RG211487 | MRGPRX1 (tGFP-tagged) - Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 5,200.00 |
|
| SC127953 | MRGPRX1 (untagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 3,600.00 |
