United States
Germany
Japan
United Kingdom
China
MRGX1 (MRGPRX1) (NM_147199) Human Untagged Clone
CAT＃: SC127953
MRGPRX1 (untagged)-Human MAS-related GPR, member X1 (MRGPRX1)
CNY 3,600.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GPCR; MGRG2; MRGX1; SNSR4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127953 sequence for NM_147199 edited (data generated by NextGen Sequencing)
ATGGATCCAACCATCTCAACCTTGGACACAGAACTGACACCAATCAACGGAACTGAGGAG ACTCTTTGCTACAAGCAGACCTTGAGCCTCACGGTGCTGACGTGCATCGTTTCCCTTGTC GGGCTGACAGGAAACGCGGTTGTGCTCTGGCTCCTGGGCTGCCGCATGCGCAGGAACGCC TTCTCCATCTACATCCTCAACTTGGCCGCAGCAGACTTCCTCTTCCTCAGCGGCCGCCTT ATATATTCCCTGTTAAGCTTCATCAGTATCCCCCATACCATCTCTAAAATCCTCTATCCT GTGATGATGTTTTCCTACTTTGCAGGCCTGAGCTTTCTGAGTGCCGTGAGCACCGAGCGC TGCCTGTCCGTCCTGTGGCCCATCTGGTACCGCTGCCACCGCCCCACACACCTGTCAGCG GTGGTGTGTGTCCTGCTCTGGGCCCTGTCCCTGCTGCGGAGCATCCTGGAGTGGATGTTA TGTGGCTTCCTGTTCAGTGGTGCTGATTCTGCTTGGTGTCAAACATCAGATTTCATCACA GTCGCGTGGCTGATTTTTTTATGTGTGGTTCTCTGTGGGTCCAGCCTGGTCCTGCTGATC AGGATTCTCTGTGGATCCCGGAAGATACCGCTGACCAGGCTGTACGTGACCATCCTGCTC ACAGTACTGGTCTTCCTCCTCTGTGGCCTGCCCTTTGGCATTCAGTTTTTCCTATTTTTA TGGATCCACGTGGACAGGGAAGTCTTATTTTGTCATGTTCATCTAGTTTCTATTTTCCTG TCCGCTCTTAACAGCAGTGCCAACCCCATCATTTACTTCTTCGTGGGCTCCTTTAGGCAG CGTCAAAATAGGCAGAACCTGAAGCTGGTTCTCCAGAGGGCTCTGCAGGACGCGTCTGAG GTGGATGAAGGTGGAGGGCAGCTTCCTGAGGAAATCCTGGAGCTGTCGGGAAGCAGATTG GAGCAGTGA Clone variation with respect to NM_147199.3 138 a=>g >OriGene 5' read for NM_147199 unedited
GTTGGCGCGCAAACTAGCGGGATTTTGTTAACACGTATTTCATGTAAGGGNCGGAACGCG CAATTCGGCATTTGCAACATGGCATCCAACCATCTCAACCTTGGGCACAGAACTGACACC AATCAACGGAACTGAGGAGACTCTTTGCTACAAGCAGACCTTGAGCCTCACGGTGCTGAC GTGCATCGTTTCCCTTGTCGGGCTGACAGGAAACGCGGTTGTGCTCTGGCTCCTGGGTTT TTGCATGCGCAGGAACGCCTTCTCCATCTACATCCTCAACTTGGCCGCAGCAGACTTCCT CTTCCTCAGCGGCCGCCTTATATATTCCCTGTTAAGCTTCATCAGTATCCCCCATACCAT CTCTAAAATCCTCTATCCTGTGATGATGTTTTCCTACTTTGCAGGCCTGAGCTTTCTGAG TGCCGTGAGCACCGAGCGCTGCCTGTCCGTCCTGTGGCCCATCTGGTACCGATGCCACCG TCCCACACACCTGTCAGCGGTGGTGTGTGTCCTGCTCTGGGCCCTGTCCCTGCTGCAGAG CATCCTGGAGTGGATGTTATGTGGCTTACTGTCCAGGGATGCTGATTCTGCTTGGTGGTC AAACATCAATTTAATCCAGTCCCCTGGCTGAATTTTTAAGGGGGGGTCCAATGTGGGTCC CAGCCTGGCCCTGGTATTCAGAATTCTTTGGGGTATCCGAAAAAACCCGTTACCAGGTTT TTCTTTACCTTTCGGGTAACAAAAAAGGGGGTTCCCCCCCTGGGGGCCCGCCCTTTTGGA ATAAAGTTTCCCCCTTTTTTAAGGGCCCCCCGGGACGGGGAAAACTTTTTTTGGGCGGGC GCCTTGTTTATTTTTCCGGGGCCGCTTTAAAAAAAAGGGCCCCCCCCTTTTTTTTTTTTT TT >OriGene 3' genomic read for NM_147199 unedited
CAGTGGCGCCATAGGGTGATGCGCAACTTCCCAGGNCCAGTAAAGCACTGGGGNAGGGTC ACAGGGTAGCCACCCGGGTATCTGTTCAGGGAAACAGCTATGACCGCGGCCGCAATCTAG AGTCGACAAGCTTGATATCGGTACCAGATCTGAATTCGGCTTTTCCTCACTGCTCCAATC TGCTTCCCGACAGCTCCAGGATTTCCTCAGGAAGCTGCCCTCCACCTTCATCCACCTCAG ACGCGTCCTGCAGAGCCCTCTGGAGAACCAGCTTCAGGTTCTGCCTATTTTGACGCTGCC TAAAGGAGCCCACGAAGAAGTAAATGATGGGGTTGGCACTGCTGTTAAGAGCGGACAGGA AAATAGAAACTAGATGAACATGACAAAATAAGACTTCCCTGTCCACGTGGATCCATAAAA ATAGGAAAAACTGAATGCCAAAGGGCAGGCCACAGAGGAGGAAGACCAGTACTGTGAGCA GGATGGTCACGTACAGCCTGGTCAGCGGTATCTTCCGGGATCCACAGAGAATCCTGATCA GCAGGACCAGGCTGGACCCACAGAGAACCACACATAAAAAAATCAGCCACGCGACTGTGA TGAAATCTGATGTTTGACACCAAGCAGAATCAGCACCACTGAACAGGAAGCCACATAACA TCCACTCCAGGATGCTCCGCAGCAGGGACAGGGCCCAGAGCAGGACACACACCACCGCTG ACAGGTGTGTGGGGGCGGTGGCAGCGGTACCATATGGGCCACAAGACGGACAGGCAGCGC TCGGTGCTCACGGCACTCAAAAAGCTCAGGCCTGNCAAAGTAGAAAACATCTCACAGATA GAAGATTTTAAAGATGTATGGGGGGATACTGATGAAAGCTACAG |
| Restriction Sites | Please inquire |
| ACCN | NM_147199 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_147199.2, NP_671732.2 |
| RefSeq Size | 1215 bp |
| RefSeq ORF | 969 bp |
| Locus ID | 259249 |
| UniProt ID | Q96LB2 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22)(PubMed:26582731). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine-induced itch, in a histamine-independent manner.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PMX-53 as a Dual CD88 Antagonist and an Agonist for Mas-Related Gene 2 (MrgX2) in Human Mast Cells
,Hariharan Subramanian, Sakeen W. Kashem, Sarah J. Collington, Hongchang Qu, John D. Lambris, and Hydar Ali,
Mol. Pharmacol., Jun 2011; 79: 1005 - 1013
[MRGPRX1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211487 | MRGPRX1 (Myc-DDK-tagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 3,600.00 |
|
| RC211487L1 | Lenti-ORF clone of MRGPRX1 (Myc-DDK-tagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 5,890.00 |
|
| RC211487L2 | Lenti-ORF clone of MRGPRX1 (mGFP-tagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 5,890.00 |
|
| RC211487L3 | Lenti-ORF clone of MRGPRX1 (Myc-DDK-tagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 5,890.00 |
|
| RC211487L4 | Lenti-ORF clone of MRGPRX1 (mGFP-tagged)-Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 6,000.00 |
|
| RG211487 | MRGPRX1 (tGFP-tagged) - Human MAS-related GPR, member X1 (MRGPRX1) |
CNY 5,200.00 |
