CHRNA3 (NM_000743) Human Tagged ORF Clone
CAT#: RC212582
- TrueORF®
CHRNA3 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1
ORF Plasmid: tGFP
"NM_000743" in other vectors (6)
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CNY 3,760.00
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BAIPRCK; LNCR2; NACHRA3; PAOD2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC212582 representing NM_000743
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCGCCGCCGCGGCTGCTGCTGCTGCTGCTGCTGT CTCTGCTGCCAGTGGCCAGGGCCTCAGAGGCTGAGCACCGTCTATTTGAGCGGCTGTTTGAAGATTACAA TGAGATCATCCGGCCTGTAGCCAACGTGTCTGACCCAGTCATCATCCATTTCGAGGTGTCCATGTCTCAG CTGGTGAAGGTGGATGAAGTAAACCAGATCATGGAGACCAACCTGTGGCTCAAGCAAATCTGGAATGACT ACAAGCTGAAGTGGAACCCCTCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCACAGAAGATCTG GAAGCCAGACATTGTGCTGTATAACAATGCTGTTGGGGATTTCCAGGTGGACGACAAGACCAAAGCCTTA CTCAAGTACACTGGGGAGGTGACTTGGATACCTCCGGCCATCTTTAAGAGCTCCTGTAAAATCGACGTGA CCTACTTCCCGTTTGATTACCAAAACTGTACCATGAAGTTCGGTTCCTGGTCCTACGATAAGGCGAAAAT CGATCTGGTCCTGATCGGCTCTTCCATGAACCTCAAGGACTATTGGGAGAGCGGCGAGTGGGCCATCATC AAAGCCCCAGGCTACAAACACGACATCAAGTACAACTGCTGCGAGGAGATCTACCCCGACATCACATACT CGCTGTACATCCGGCGCCTGCCCTTGTTCTACACCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTT CCTCACTGTGCTCGTCTTCTACCTGCCCTCCGACTGCGGTGAGAAGGTGACCCTGTGCATTTCTGTCCTC CTCTCCCTGACGGTGTTTCTCCTGGTGATCACTGAGACCATCCCTTCCACCTCGCTGGTCATCCCCCTGA TTGGAGAGTACCTCCTGTTCACCATGATTTTTGTAACCTTGTCCATCGTCATCACCGTCTTCGTGCTCAA CGTGCACTACAGAACCCCGACGACACACACAATGCCCTCATGGGTGAAGACTGTATTCTTGAACCTGCTC CCCAGGGTCATGTTCATGACCAGGCCAACAAGCAACGAGGGCAACGCTCAGAAGCCGAGGCCCCTCTACG GTGCCGAGCTCTCAAATCTGAATTGCTTCAGCCGCGCAGAGTCCAAAGGCTGCAAGGAGGGCTACCCCTG CCAGGACGGGATGTGTGGTTACTGCCACCACCGCAGGATAAAAATCTCCAATTTCAGTGCTAACCTCACG AGAAGCTCTAGTTCTGAATCTGTTGATGCTGTGCTGTCCCTCTCTGCTTTGTCACCAGAAATCAAAGAAG CCATCCAAAGTGTCAAGTATATTGCTGAAAATATGAAAGCACAAAATGAAGCCAAAGAGATTCAAGATGA TTGGAAGTATGTTGCCATGGTGATTGATCGTATTTTTCTGTGGGTTTTCACCCTGGTGTGCATTCTAGGG ACAGCAGGATTGTTTCTGCAACCCCTGATGGCCAGGGAAGATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212582 representing NM_000743
Red=Cloning site Green=Tags(s) MGSGPLSLPLALSPPRLLLLLLLSLLPVARASEAEHRLFERLFEDYNEIIRPVANVSDPVIIHFEVSMSQ LVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKAL LKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAII KAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVL LSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLL PRVMFMTRPTSNEGNAQKPRPLYGAELSNLNCFSRAESKGCKEGYPCQDGMCGYCHHRRIKISNFSANLT RSSSSESVDAVLSLSALSPEIKEAIQSVKYIAENMKAQNEAKEIQDDWKYVAMVIDRIFLWVFTLVCILG TAGLFLQPLMAREDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000743 |
ORF Size | 1515 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000743.2, NP_000734.2 |
RefSeq Size | 3020 bp |
RefSeq ORF | 1518 bp |
Locus ID | 1136 |
UniProt ID | P32297 |
Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
MW | 57.3 kDa |
Gene Summary | This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
The regulatory effect of Alisma Rhizomes and their triterpenoids on a3ß4 nicotinic acetylcholine receptor activity
,Lee, SB;Noh, S;Yeom, HD;Kim, H;Kim, W;Kim, YS;Bae, H;Lee, J;,
Oriental Pharmacy and Experimental Medicine
[CHRNA3]
|
Novel cholinergic peptides SLURP-1 and -2 regulate epithelialization of cutaneous and oral wounds
,null,
Wound Repair and Regeneration
,PubMed ID 22168155
[CHRNA3]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212582L1 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, Myc-DDK-tagged |
CNY 6,160.00 |
|
RC212582L2 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC212582L3 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC212582L4 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG212582 | CHRNA3 (tGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1 |
CNY 5,360.00 |
|
SC122160 | CHRNA3 (untagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1 |
CNY 3,768.00 |