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CHRNA3 (NM_000743) Human Untagged Clone
CAT＃: SC122160
CHRNA3 (untagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1
CNY 3,768.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BAIPRCK; LNCR2; NACHRA3; PAOD2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC122160 sequence for NM_000743 edited (data generated by NextGen Sequencing)
ATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCGCCGCCGCGGCTGCTGCTGCTG CTGCTGCTGTCTCTGCTGCCAGTGGCCAGGGCCTCAGAGGCTGAGCACCGTCTATTTGAG CGGCTGTTTGAAGATTACAATGAGATCATCCGGCCTGTAGCCAACGTGTCTGACCCAGTC ATCATCCATTTCGAGGTGTCCATGTCTCAGCTGGTGAAGGTGGATGAAGTAAACCAGATC ATGGAGACCAACCTGTGGCTCAAGCAAATCTGGAATGACTACAAGCTGAAGTGGAACCCC TCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCACAGAAGATCTGGAAGCCAGAC ATTGTGCTGTATAACAATGCTGTTGGGGATTTCCAGGTGGACGACAAGACCAAAGCCTTA CTCAAGTACACTGGGGAGGTGACTTGGATACCTCCGGCCATCTTTAAGAGCTCCTGTAAA ATCGACGTGACCTACTTCCCGTTTGATTACCAAAACTGTACCATGAAGTTCGGTTCCTGG TCCTACGATAAGGCGAAAATCGATCTGGTCCTGATCGGCTCTTCCATGAACCTCAAGGAC TATTGGGAGAGCGGCGAGTGGGCCATCATCAAAGCCCCAGGCTACAAACACGACATCAAG TACAACTGCTGCGAGGAGATCTACCCCGACATCACATACTCGCTGTACATCCGGCGCCTG CCCTTGTTCTACACCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTTCCTCACTGTG CTCGTCTTCTACCTGCCCTCCGACTGCGGTGAGAAGGTGACCCTGTGCATTTCTGTCCTC CTCTCCCTGACGGTGTTTCTCCTGGTGATCACTGAGACCATCCCTTCCACCTCGCTGGTC ATCCCCCTGATTGGAGAGTACCTCCTGTTCACCATGATTTTTGTAACCTTGTCCATCGTC ATCACCGTCTTCGTGCTCAACGTGCACTACAGAACCCCGACGACACACACAATGCCCTCA TGGGTGAAGACTGTATTCTTGAACCTGCTCCCCAGGGTCATGTTCATGACCAGGCCAACA AGCAACGAGGGCAACGCTCAGAAGCCGAGGCCCCTCTACGGTGCCGAGCTCTCAAATCTG AATTGCTTCAGCCGCGCAGAGTCCAAAGGCTGCAAGGAGGGCTACCCCTGCCAGGACGGG ATGTGTGGTTACTGCCACCACCGCAGGATAAAAATCTCCAATTTCAGTGCTAACCTCACG AGAAGCTCTAGTTCTGAATCTGTTGATGCTGTGCTGTCCCTCTCTGCTTTGTCACCAGAA ATCAAAGAAGCCATCCAAAGTGTCAAGTATATTGCTGAAAATATGAAAGCACAAAATGAA GCCAAAGAGATTCAAGATGATTGGAAGTATGTTGCCATGGTGATTGATCGTATTTTTCTG TGGGTTTTCACCCTGGTGTGCATTCTAGGGACAGCAGGATTGTTTCTGCAACCCCTGATG GCCAGGGAAGATGCATAA Clone variation with respect to NM_000743.4 291 a=>g >OriGene 5' read for NM_000743 unedited
ATCAAATATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTCCTCC CGCGGNGTCCGAGGGGCGCTGGNAAACCCAGCGGCGGCGAAGCGGAGAGGAGCCCCGCGC GTCTCCGCCCGCACGGCTCCAGGTCTGGGGTCTGCGCTGGAGCCGCGCGGGGAGAGGCCG TCTCTGCGACCGCCGCGCCCGCTCCCGACCGTCCGGGTCCGCGGCCAGCCCGGCCACCAG CCATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCGCCGCCGCGGCTGCTGCTGC TGCTGCTGCTGTCTCTGCTGCCAGTGGCCAGGGCCTCAGAGGCTGAGCACCGTCTATTTG AGCGGCTGTTTGAAGATTACAATGAGATCATCCGGCCTGTAGCCAACGTGTCTGACCCAG TCATCATCCATTTCGAGGTGTCCATGTCTCAGCTGGTGAAGGTGGATGAAGTAAACCAGA TCATGGAGACCAACCTGTGGCTCAAGCAAATCTGGAATGACTACAAGCTGAAGTGGAACC CCTCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCACAGAAGATCTGGAAGCCAG ACATTGTGCTGTATAACAATGCTGTTGGGGATTTCCAGGTGGACGANCAGACCAAAGCCT TTACTCAGTACACTGGGGGAGGTGACTTGGGATACCTNCGGCCCATCTTNAGAGCTCCTG GTAAATCGACGTGACCTACTTCCCGTTTGATTACCCAAAACTGTGCCCTGAAATTCGGGG TCCTGGTGCCTACAATAAGGCAAAAATCGATCCTGGTCCTGATCCGGCTCTTCCATGACC CTCCAGAACCTATGGGAGAAGCGGCGAATGGGCCCATTATAAAACCCCCGGGCTACAAAC ACCAAATTAGGTCCAACTGGTTGGAAGGGAATCTTCCCGCT |
| Restriction Sites | Please inquire |
| ACCN | NM_000743 |
| Insert Size | 1518 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000743.2, NP_000734.2 |
| RefSeq Size | 3020 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 1136 |
| UniProt ID | P32297 |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| Gene Summary | This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
NACHO Engages N-Glycosylation ER Chaperone Pathways for α7 Nicotinic Receptor Assembly
,Kweon, HJ;Gu, S;Witham, E;Dhara, M;Yu, H;Mandon, ED;Jawhari, A;Bredt, DS;,
Cell Rep
,PubMed ID 32783947
[CHRNA3]
|
|
α6-Containing Nicotinic Acetylcholine Receptor Reconstitution Involves Mechanistically Distinct Accessory Components
,Gu, S;Matta, JA;Davini, WB;Dawe, GB;Lord, B;Bredt, DS;,
Cell Rep
,PubMed ID 30673609
[CHRNA3]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212582 | CHRNA3 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1 |
CNY 3,760.00 |
|
| RC212582L1 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, Myc-DDK-tagged |
CNY 6,160.00 |
|
| RC212582L2 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC212582L3 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212582L4 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG212582 | CHRNA3 (tGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1 |
CNY 5,360.00 |
