PRAK (MAPKAPK5) (NM_003668) Human Tagged ORF Clone
CAT#: RC216715
- TrueORF®
MAPKAPK5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1
ORF Plasmid: tGFP
"NM_003668" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 3,990.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MAPKAP-K5; MK-5; MK5; PRAK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216715 representing NM_003668
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAGGAGAGCGACATGGACAAAGCCATCAAGGAAACTTCCATTTTAGAAGAATACAGTATCAATT GGACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGTTAGAGTCTGTGTAAAGAAATCTACTCAAGAACG GTTTGCGCTGAAAATTCTTCTTGATCGTCCAAAAGCTAGAAATGAGGTACGTCTGCACATGATGTGTGCC ACACACCCAAACATAGTTCAGATTATTGAAGTGTTTGCTAACAGTGTCCAGTTTCCCCATGAGTCCAGCC CTAGGGCCCGACTCTTAATTGTAATGGAGATGATGGAAGGGGGAGAGCTATTTCACAGAATCAGCCAGCA CCGGCACTTTACAGAGAAGCAAGCCAGCCAAGTAACAAAGCAGATAGCTTTGGCTCTGCGGCACTGTCAC TTGTTAAACATTGCGCACAGAGACCTCAAGCCTGAAAATCTGCTTTTTAAGGATAACTCTTTGGATGCCC CAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTGATGACACCCCAGTTCACCCC TTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCT ACCTCACCGACGCCCTACACTTACAACAAGAGCTGTGACTTGTGGTCCCTAGGGGTGATTATCTATGTGA TGCTGTGCGGATACCCTCCTTTTTACTCCAAACACCACAGCCGGACTATCCCAAAGGATATGCGAAGAAA GATCATGACAGGCAGTTTTGAGTTCCCAGAGGAAGAGTGGAGTCAGATCTCAGAGATGGCCAAAGATGTT GTGAGGAAGCTCCTGAAGGTCAAACCGGAGGAGAGACTCACCATCGAGGGAGTGCTGGACCACCCCTGGC TCAATTCCACCGAGGCCCTGGATAATGTGCTGCCTTCTGCTCAGCTGATGATGGACAAGGCAGTGGTTGC AGGAATCCAGCAGGCTCACGCGGAACAGTTGGCCAACATGAGAATCCAGGATCTGAAAGTCAGCCTCAAA CCCCTGCACTCAGTGAACAACCCCATTCTGCGGAAGAGGAAGTTACTTGGCACCAAGCCAAAGGACAGTG TCTATATCCACGACCATGAGAATGGAGCCGAGGATTCCAATGTTGCCTTGGAAAAACTCCGAGATGTGAT TGCTCAGTGTATTCTCCCCCAGGCTGGAGAGAATGAAGATGAGAAACTGAATGAAGTAATGCAGGAGGCT TGGAAGTATAACCGGGAATGCAAACTCCTAAGAGATACTCTGCAGAGCTTCAGCTGGAATGGTCGTGGAT TCACAGATAAAGTAGATCGACTAAAACTGGCAGAAATTGTGAAGCAGGTGATAGAAGAGCAAACCACGTC CCACGAATCCCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216715 representing NM_003668
Red=Cloning site Green=Tags(s) MSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCA THPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCH LLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIP TSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRRKIMTGSFEFPEEEWSQISEMAKDV VRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLK PLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGENEDEKLNEVMQEA WKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEEQTTSHESQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003668 |
ORF Size | 1413 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003668.4 |
RefSeq Size | 2060 bp |
RefSeq ORF | 1416 bp |
Locus ID | 8550 |
UniProt ID | Q8IW41 |
Domains | pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | MAPK signaling pathway |
MW | 53.9 kDa |
Gene Summary | The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216715L1 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC216715L2 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC216715L3 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC216715L4 | Lenti ORF clone of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG216715 | MAPKAPK5 (tGFP-tagged) - Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1 |
CNY 5,256.00 |
|
SC109943 | MAPKAPK5 (untagged)-Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1 |
CNY 2,950.00 |
|
SC323359 | MAPKAPK5 (untagged)-Kinase deficient mutant (K45M) of Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1 |
CNY 3,656.00 |