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ARHGEF7 (NM_145735) Human Tagged ORF Clone
CAT＃: RC218798
ARHGEF7 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2
ORF Plasmid: tGFP
"NM_145735" in other vectors (6)
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CNY 5,728.00
CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218798 representing NM_145735
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCCGCCGAGCAAACCGTTACGTGGCTCATCACTCTGGGGGTGCTGGAGTCGCCCAAAAAAACCA TCTCGGACCCGGAGGGCTTTCTGCAGGCGTCGCTGAAGGATGGGGTGGTCCTCTGCAGGCTGCTGGAGCG CCTGCTCCCCGGGACCATCGAGAAAGTCTACCCCGAGCCCCGGAGCGAGAGCGAGTGCCTGAGCAACATC CGCGAGTTCCTGCGCGGCTGCGGGGCTTCCCTGCGGCTGGAGACGTTTGATGCAAATGATTTGTATCAGG GGCAGAATTTTAACAAGGTCCTCAGTTCCTTAGTGACTCTAAATAAAGTAACAGCAGACATCGGGCTGGG GAGTGACTCCGTGTGTGCCCGGCCCTCGTCTCACCGCATAAAGTCTTTTGACTCCCTTGGATCACAGTCT TTGCACACTCGGACTTCAAAACTGTTCCAGGGCCAGTATCGGAGTTTGGACATGACCGATAATAGCAACA ATCAACTGGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACCAATGAGGACGAGCTTTCCTTCTCAAAAGG AGACGTCATCCATGTCACCCGTGTGGAAGAGGGAGGCTGGTGGGAGGGCACACTCAACGGCCGGACCGGC TGGTTCCCCAGCAACTACGTGCGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCTCCCAAATCAGGAACAC TGAAGAGCCCTCCCAAAGGATTTGATACGACTGCCATAAACAAAAGCTATTACAATGTGGTGCTACAGAA TATTTTAGAAACAGAAAATGAATATTCTAAAGAACTTCAGACTGTGCTTTCAACGTACCTACGGCCATTG CAGACCAGTGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATGGGAAATCTAGAAGAAATATGTTCTT TCCAGCAAATGCTCGTACAGTCTTTAGAAGAATGCACCAAGTTGCCCGAAGCTCAGCAGAGAGTCGGAGG CTGCTTTTTAAACCTGATGCCACAGATGAAAACCCTGTACCTCACGTATTGTGCCAATCACCCTTCTGCA GTGAATGTCCTCACGGAACACAGTGAGGAGTTGGGGGAGTTCATGGAGACCAAAGGTGCCAGCAGCCCTG GGATTCTCGTGCTGACCACGGGCCTGAGCAAACCCTTCATGCGCCTGGATAAATACCCTACGCTGCTCAA AGAGCTCGAGAGACACATGGAGGATTATCATACAGATAGACAAGATATTCAAAAATCCATGGCTGCCTTC AAAAACCTTTCAGCCCAATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAGCTGCAGATCCTGACGGAAG CCATCCGGAACTGGGAGGGCGATGACATTAAAACTCTGGGCAACGTCACTTACATGTCCCAGGTCCTGAT TCAGTGTGCCGGAAGTGAGGAAAAGAATGAAAGATATCTTCTACTCTTCCCAAATGTTTTGCTAATGTTG TCTGCCAGTCCTAGGATGAGTGGCTTTATCTATCAGGGAAAGCTTCCAACGACAGGAATGACAATCACAA AGCTTGAGGACAGTGAAAATCATAGAAATGCATTTGAAATATCAGGGAGCATGATTGAGCGGATATTAGT GTCGTGCAACAACCAGCAGGATCTGCAGGAATGGGTGGAGCACCTACAGAAGCAAACGAAGGTCACGTCT GTGGGAAACCCCACCATAAAGCCTCATTCAGTGCCATCTCATACCCTCCCCTCCCACCCGGTCACTCCGT CCAGCAAGCACGCAGACAGCAAGCCCGCGCCGCTGACGCCCGCCTACCACACGCTGCCCCACCCCTCCCA CCACGGCACCCCGCACACCACCATCAACTGGGGACCCCTGGAGCCTCCGAAAACACCCAAGCCCTGGAGC CTGAGCTGCCTGCGGCCCGCGCCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTTAGTA AGAGCCCTAAGACCATGAAAAAGCTGCTGCCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTT CGCGTCCCGGAAAAGCACAGCTGCTTTGGAAGAAGATGCTCAGATTCTGAAAGTCATTGAAGCTTACTGC ACCAGCGCCAAAACAAGGCAAACACTCAATTCAACATGGCAAGGCACTGACCTGATGCATAATCACGTCT TGGCTGATGATGACCAACCAAGCCTAGACTCCCTGGGGCGTCGCAGTAGCCTTTCTCGTTTGGAGCCTTC AGACCTCTCGGAAGACTCTGACTATGACAGTATATGGACAGCCCATAGTTACAGAATGGGTTCTACATCT CGTAAGAGCTGTTGCTCATATATCTCTCACCAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218798 representing NM_145735
Red=Cloning site Green=Tags(s) MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRLLERLLPGTIEKVYPEPRSESECLSNI REFLRGCGASLRLETFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQS LHTRTSKLFQGQYRSLDMTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTG WFPSNYVREVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPL QTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTKLPEAQQRVGGCFLNLMPQMKTLYLTYCANHPSA VNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHTDRQDIQKSMAAF KNLSAQCQEVRKRKELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLML SASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTS VGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWS LSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYC TSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTS RKSCCSYISHQN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145735 |
| ORF Size | 2346 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145735.3 |
| RefSeq Size | 5304 bp |
| RefSeq ORF | 2349 bp |
| Locus ID | 8874 |
| UniProt ID | Q14155 |
| Domains | RhoGEF, SH3, CH, PH |
| Protein Pathways | Regulation of actin cytoskeleton |
| MW | 87.6 kDa |
| Gene Summary | This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218798L1 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, Myc-DDK-tagged |
CNY 8,128.00 |
|
| RC218798L2 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, mGFP tagged |
CNY 10,170.00 |
|
| RC218798L3 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, Myc-DDK-tagged |
CNY 10,170.00 |
|
| RC218798L4 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, mGFP tagged |
CNY 10,170.00 |
|
| RG218798 | ARHGEF7 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2 |
CNY 9,120.00 |
|
| SC110008 | ARHGEF7 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2 |
CNY 5,736.00 |
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