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ARHGEF7 (NM_145735) Human Untagged Clone
CAT＃: SC110008
ARHGEF7 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2
CNY 5,736.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_145735, the custom clone sequence may differ by one or more nucleotides
ATGAATTCCGCCGAGCAAACCGTTACGTGGCTCATCACTCTGGGGGTGCTGGAGTCGCCCAAAAAAACCA TCTCGGACCCGGAGGGCTTTCTGCAGGCGTCGCTGAAGGATGGGGTGGTCCTCTGCAGGCTGCTGGAGCG CCTGCTCCCCGGGACCATCGAGAAAGTCTACCCCGAGCCCCGGAGCGAGAGCGAGTGCCTGAGCAACATC CGCGAGTTCCTGCGCGGCTGCGGGGCTTCCCTGCGGCTGGAGACGTTTGATGCAAATGATTTGTATCAGG GGCAGAATTTTAACAAGGTCCTCAGTTCCTTAGTGACTCTAAATAAAGTAACAGCAGACATCGGGCTGGG GAGTGACTCCGTGTGTGCCCGGCCCTCGTCTCACCGCATAAAGTCTTTTGACTCCCTTGGATCACAGTCT TTGCACACTCGGACTTCAAAACTGTTCCAGGGCCAGTATCGGAGTTTGGACATGACCGATAATAGCAACA ATCAACTGGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACCAATGAGGACGAGCTTTCCTTCTCAAAAGG AGACGTCATCCATGTCACCCGTGTGGAAGAGGGAGGCTGGTGGGAGGGCACACTCAACGGCCGGACCGGC TGGTTCCCCAGCAACTACGTGCGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCTCCCAAATCAGGAACAC TGAAGAGCCCTCCCAAAGGATTTGATACGACTGCCATAAACAAAAGCTATTACAATGTGGTGCTACAGAA TATTTTAGAAACAGAAAATGAATATTCTAAAGAACTTCAGACTGTGCTTTCAACGTACCTACGGCCATTG CAGACCAGTGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATGGGAAATCTAGAAGAAATATGTTCTT TCCAGCAAATGCTCGTACAGTCTTTAGAAGAATGCACCAAGTTGCCCGAAGCTCAGCAGAGAGTCGGAGG CTGCTTTTTAAACCTGATGCCACAGATGAAAACCCTGTACCTCACGTATTGTGCCAATCACCCTTCTGCA GTGAATGTCCTCACGGAACACAGTGAGGAGTTGGGGGAGTTCATGGAGACCAAAGGTGCCAGCAGCCCTG GGATTCTCGTGCTGACCACGGGCCTGAGCAAACCCTTCATGCGCCTGGATAAATACCCTACGCTGCTCAA AGAGCTCGAGAGACACATGGAGGATTATCATACAGATAGACAAGATATTCAAAAATCCATGGCTGCCTTC AAAAACCTTTCAGCCCAATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAGCTGCAGATCCTGACGGAAG CCATCCGGAACTGGGAGGGCGATGACATTAAAACTCTGGGCAACGTCACTTACATGTCCCAGGTCCTGAT TCAGTGTGCCGGAAGTGAGGAAAAGAATGAAAGATATCTTCTACTCTTCCCAAATGTTTTGCTAATGTTG TCTGCCAGTCCTAGGATGAGTGGCTTTATCTATCAGGGAAAGCTTCCAACGACAGGAATGACAATCACAA AGCTTGAGGACAGTGAAAATCATAGAAATGCATTTGAAATATCAGGGAGCATGATTGAGCGGATATTAGT GTCGTGCAACAACCAGCAGGATCTGCAGGAATGGGTGGAGCACCTACAGAAGCAAACGAAGGTCACGTCT GTGGGAAACCCCACCATAAAGCCTCATTCAGTGCCATCTCATACCCTCCCCTCCCACCCGGTCACTCCGT CCAGCAAGCACGCAGACAGCAAGCCCGCGCCGCTGACGCCCGCCTACCACACGCTGCCCCACCCCTCCCA CCACGGCACCCCGCACACCACCATCAACTGGGGACCCCTGGAGCCTCCGAAAACACCCAAGCCCTGGAGC CTGAGCTGCCTGCGGCCCGCGCCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTTAGTA AGAGCCCTAAGACCATGAAAAAGCTGCTGCCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTT CGCGTCCCGGAAAAGCACAGCTGCTTTGGAAGAAGATGCTCAGATTCTGAAAGTCATTGAAGCTTACTGC ACCAGCGCCAAAACAAGGCAAACACTCAATTCAACATGGCAAGGCACTGACCTGATGCATAATCACGTCT TGGCTGATGATGACCAACCAAGCCTAGACTCCCTGGGGCGTCGCAGTAGCCTTTCTCGTTTGGAGCCTTC AGACCTCTCGGAAGACTCTGACTATGACAGTATATGGACAGCCCATAGTTACAGAATGGGTTCTACATCT CGTAAGAGCTGTTGCTCATATATCTCTCACCAGAACTAA >OriGene 5' read for NM_145735 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGNAGCCGCTCCTGAGAAGCGCCTG ACAGCGGGCCGGGGCGCACGGAGAAGCGGGCCGGGCCGGACCTGCTGGGCCGCGCCGAGC CAATCGCCGGCGCCGGCCGCTCGATGGGCGAGGCGGCGGCGGCGGCGGCGGGGGCCGCGG GCCGGGCCGCCGCTCCGAGGTGAAGGCGCGCGCCCCTCCCCGCCTGCCTCCCGGGCCGCA GCGATGAATTCCGCCGAGCAAACCGTTACGTGGCTCATCACTCTGGGGGTGCTGGAGTCG CCCAAAAAAACCATCTCGGACCCGGAGGGCTTTCTGCAGGCGTCGCTGAAGGATGGGGTG GTCCTCTGCAGGCTGCTGGAGCGCCTGCTCCCCGGGACCATCGAGAAAGTCTACCCCGAG CCCCGGAGCGAGAGCGAGTGCCTGAGCAACATCCGCGAGTTCCTGCGCGGCTGCGGGGCT TCCCTGCGGCTGGAGACGTTTGATGCAAATGATTTGTATCAGGGGCAGAATTTTAACAAG GTCCTCAGTTCCTTAGTGACTCTAAATAAAGTAACAGCAGACATCGGGCTGGGGAGTGAC TCCGTGTGTGCCCGGCCCTCGTCTCACCGCATAAAGTCTTTTGACTCCCTTGGATCACAG TCTTTGCACACTCGGACTTCAAAACTGTTCCAGGGCCAGTATCGGNAGTTTGGACATGAC CGANNTATAGCACAATCAACTGGTAGTAAGAGCCAAAGTTAACTTCCAGCAGACCAAGGG AGACGAGCNTTTCCTTCTCAAAAGGAGACGTCATCCATGTCACCCGTGTTGAANAAGGNA GCTGGTTGGNAGGCACACTCACGGGNCGGACCGGGTGGTTCCCCNCAACTACGTGCGCAA GGTCAGGCCANCGAAAAC >OriGene 3' read for NM_145735 unedited
CAGCTATGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATAAC GAGCCCATGGCAACCTAGTAATTCTTCAATGAACTTCAATATCCCATTACAATGAGGCAT TGCGGTTTTATACTATGGGGTTGCTGTAAATGGAGTTTCACTGCAGCTGGTTGCTGAAAA TAATGATGTGTCCATGGCTCTTCCAGACAACATAAGTATGGATGTCAGGGGTGGGGAGCC GCTGGATCTGAAGCCACAGCACTTGACTTTGTAAGTACTCCAAATGCCTCTACTACAGCC CAGTAGTCTACACTTGCAAAGGCTGAATGCTTTATCTTTCGGGCTTTAAACTGTTAATGT ATACATCTTACTGTAGACCCTTACAGGATCGCTCTGTGCCTCATCCCAGAATTCACTTGC CTTTCATGCCATTTCGGATTGCCTACCCCCGCCCACCCCCGACCCCTCGTACACCAGCCC TGACTTTCGAAAGCACACCTTACCTCTCCCCGGCCACCCCCCAACACTCTCACCACCTCC CCCCCGTAAGGTCCACGCTAGTTGGTTACAAACCCTCACGTACCCCCTCCCGCCCCACTG CTCCAGCTTCGCCCCCACCCCCGCTCCCCCCCCCCCCCGGCACAAGCGCGTGCATTTGGC TTTCTCACAATCCCGCCCGCCGTCTCCCTCCCCCCCCCTCCCCCCTCCCCCATATCTCCG TTTCCCCACCTCTACCACATCAGTTCCCATTTCTCTATCGCCCCCGCCGCCCATCAACAC ACCCCCCTTCTCCACCCTCCCCTTTCGAGGCGACCAAAACAATACTTAAAACGCCCCCTC CCCCCGCCCCTCTTCCCCACTTCTCATCCGCCAAGGTCGCCACCCCCTCCCCCCTCTCCC CTCATACCCCCCTCCCCCTCCCGCCCCCCCCATATTCCCCTTCTTGCCCTCACCCACTCT CGTACACGCCCGCCTCCGCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_145735 |
| Insert Size | 3890 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145735.1, NP_663788.1 |
| RefSeq Size | 5304 bp |
| RefSeq ORF | 2349 bp |
| Locus ID | 8874 |
| UniProt ID | Q14155 |
| Domains | RhoGEF, SH3, CH, PH |
| Protein Pathways | Regulation of actin cytoskeleton |
| Gene Summary | This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 3. The resulting protein (isoform b) is shorter than isoform c. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218798 | ARHGEF7 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2 |
CNY 5,728.00 |
|
| RC218798L1 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, Myc-DDK-tagged |
CNY 8,128.00 |
|
| RC218798L2 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, mGFP tagged |
CNY 10,170.00 |
|
| RC218798L3 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, Myc-DDK-tagged |
CNY 10,170.00 |
|
| RC218798L4 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, mGFP tagged |
CNY 10,170.00 |
|
| RG218798 | ARHGEF7 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2 |
CNY 9,120.00 |
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