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Prickle 2 (PRICKLE2) (NM_198859) Human Tagged ORF Clone
CAT＃: RC219763
- TrueORF®
PRICKLE2 (Myc-DDK-tagged)-Human prickle homolog 2 (Drosophila) (PRICKLE2)
ORF Plasmid: tGFP
"NM_198859" in other vectors (4)
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CNY 6,184.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EPM5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219763 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGACAGTGATGCCGCTGGAGATGGAGAAGACCATCAGCAAACTCATGTTTGACTTTCAGAGGAACT CGACCTCAGATGATGACTCAGGCTGTGCTTTGGAAGAGTATGCCTGGGTCCCGCCGGGTCTGAAGCCTGA ACAGGTACACCAGTACTATAGCTGTCTCCCAGAAGAGAAAGTCCCTTATGTCAACAGTCCTGGAGAGAAA CTGCGAATCAAGCAGCTACTACACCAGCTGCCGCCACATGACAATGAGGTTCGATATTGCAACTCCCTGG ATGAGGAAGAGAAGAGGGAGCTGAAGCTTTTCAGCAGCCAGAGGAAACGCGAAAACTTGGGCCGCGGGAA TGTCAGGCCTTTCCCAGTCACCATGACAGGAGCTATTTGTGAACAGTGCGGAGGCCAGATCAATGGTGGA GACATCGCTGTGTTTGCGTCACGCGCTGGCCACGGCGTTTGCTGGCACCCGCCGTGCTTCGTATGCACTG TCTGCAATGAGCTCCTGGTGGATCTGATCTACTTTTACCAAGATGGGAAGATATACTGTGGCAGGCACCA TGCTGAGTGCCTGAAGCCGCGCTGTGCTGCCTGCGATGAGATCATCTTTGCAGATGAATGCACAGAAGCT GAGGGGCGACACTGGCACATGAAACACTTTTGCTGCTTCGAGTGTGAGACAGTGCTGGGCGGCCAGCGCT ACATCATGAAGGAGGGAAGACCCTACTGTTGCCACTGCTTCGAGTCCTTGTATGCAGAATATTGTGACAC CTGTGCCCAACATATAGGTATCGACCAAGGTCAAATGACCTATGACGGCCAACACTGGCATGCCACTGAG ACCTGTTTCTGCTGTGCTCACTGCAAGAAATCCCTCCTGGGGCGGCCATTCCTCCCGAAGCAGGGCCAGA TATTCTGCTCACGGGCCTGCAGTGCTGGGGAAGACCCCAATGGTTCTGACTCCTCTGATTCCGCCTTCCA GAACGCCAGGGCCAAGGAGTCCCGGCGCAGTGCCAAAATTGGCAAGAACAAGGGCAAGACGGAGGAGCCC ATGCTGAACCAGCACAGCCAGCTGCAAGTGAGTTCTAACCGGCTGTCAGCCGACGTAGACCCCCTGTCAC TGCAGATGGACATGCTCAGCCTGTCCAGCCAGACACCCAGCCTCAACCGGGACCCCATCTGGAGGAGCCG GGAAGAGCCCTACCATTATGGGAACAAGATGGAGCAGAACCAGACCCAGAGCCCTCTGCAGCTCCTCAGC CAGTGCAACATCAGAACTTCCTACAGTCCAGGAGGGCAAGGGGCTGGGGCCCAGCCCGAAATGTGGGGCA AGCACTTCAGCAACCCCAAAAGGAGCTCGTCACTGGCCATGACAGGACATGCTGGCAGCTTCATCAAGGA ATGCCGAGAAGACTATTACCCGGGGAGGCTGAGATCTCAGGAGAGCTACAGTGATATGTCTAGTCAGAGT TTCAGTGAGACCCGAGGCAGCATCCAAGTCCCCAAATATGAGGAGGAAGAGGAAGAGGAAGGGGGCTTGT CCACTCAGCAGTGTCGGACCCGTCATCCCATCAGTTCCCTGAAATACACAGAGGACATGACGCCCACAGA GCAGACCCCTCGGGGCTCCATGGAATCCCTGGCCCTGTCTAATGCAACAGGCCTCTCTGCTGATGGTGGT GCCAAGCGCCAGGAGCACCTATCCCGATTTTCCATGCCTGACCTCAGCAAAGACTCTGGAATGAATGTGT CTGAGAAGCTGAGCAACATGGGCACTCTTAACTCGTCCATGCAGTTCCGGAGCGCAGAGTCAGTTCGCAG CCTGCTCTCTGCCCAGCAGTACCAGGAGATGGAGGGAAACCTCCACCAGCTCAGCAACCCCATTGGCTAC AGAGACCTGCAGTCCCACGGAAGGATGCATCAGAGCTTTGATTTTGATGGAGGGATGGCGGGCAGCAAGC TGCCAGGGCAGGAGGGCGTGAGGATCCAGCCCATGAGTGAACGCACCCGGAGAAGAGCTACTTCACGCGA CGACAACCGCCGTTTCCGACCTCACAGGTCCAGGCGTTCCCGACGCTCTCGCTCCGACAACGCCCTCCAC CTGGCCAGCGAACGCGAGGCCATCTCCCGGTTAAAAGATAGGCCCCCTCTGAGAGCCAGGGAGGACTATG ACCAATTTATGCGCCAGCGGAGCTTCCAGGAGAGCATGGGGCATGGGTCCCGGAGGGACCTGTACGGCCA GTGCCCTAGGACTGTGTCGGACCTGGCTTTGCAGAATGCCTTTGGGGACCGCTGGGGACCCTACTTCGCC GAGTATGATTGGTGTTCCACCTGCTCCTCCTCTTCAGAGTCTGACAACGAGGGCTATTTCCTAGGAGAAC CCATCCCCCAGCCAGCGCGCCTGCGATACGTCACAAGCGATGAGCTGCTGCACAAATACAGCTCCTACGG CCTCCCCAAATCTTCCACATTAGGTGGCAGAGGACAGTTGCACAGCAGGAAAAGACAGAAGAGCAAAAAC TGTATCATTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219763 protein sequence
Red=Cloning site Green=Tags(s) MVTVMPLEMEKTISKLMFDFQRNSTSDDDSGCALEEYAWVPPGLKPEQVHQYYSCLPEEKVPYVNSPGEK LRIKQLLHQLPPHDNEVRYCNSLDEEEKRELKLFSSQRKRENLGRGNVRPFPVTMTGAICEQCGGQINGG DIAVFASRAGHGVCWHPPCFVCTVCNELLVDLIYFYQDGKIYCGRHHAECLKPRCAACDEIIFADECTEA EGRHWHMKHFCCFECETVLGGQRYIMKEGRPYCCHCFESLYAEYCDTCAQHIGIDQGQMTYDGQHWHATE TCFCCAHCKKSLLGRPFLPKQGQIFCSRACSAGEDPNGSDSSDSAFQNARAKESRRSAKIGKNKGKTEEP MLNQHSQLQVSSNRLSADVDPLSLQMDMLSLSSQTPSLNRDPIWRSREEPYHYGNKMEQNQTQSPLQLLS QCNIRTSYSPGGQGAGAQPEMWGKHFSNPKRSSSLAMTGHAGSFIKECREDYYPGRLRSQESYSDMSSQS FSETRGSIQVPKYEEEEEEEGGLSTQQCRTRHPISSLKYTEDMTPTEQTPRGSMESLALSNATGLSADGG AKRQEHLSRFSMPDLSKDSGMNVSEKLSNMGTLNSSMQFRSAESVRSLLSAQQYQEMEGNLHQLSNPIGY RDLQSHGRMHQSFDFDGGMAGSKLPGQEGVRIQPMSERTRRRATSRDDNRRFRPHRSRRSRRSRSDNALH LASEREAISRLKDRPPLRAREDYDQFMRQRSFQESMGHGSRRDLYGQCPRTVSDLALQNAFGDRWGPYFA EYDWCSTCSSSSESDNEGYFLGEPIPQPARLRYVTSDELLHKYSSYGLPKSSTLGGRGQLHSRKRQKSKN CIIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198859 |
| ORF Size | 2532 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198859.2 |
| RefSeq Size | 8322 bp |
| RefSeq ORF | 2535 bp |
| Locus ID | 166336 |
| UniProt ID | Q7Z3G6 |
| Protein Pathways | Wnt signaling pathway |
| MW | 95.6 kDa |
| Gene Summary | This gene encodes a homolog of Drosophila prickle. The exact function of this gene is not known, however, studies in mice suggest that it may be involved in seizure prevention. Mutations in this gene are associated with progressive myoclonic epilepsy type 5. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219763L3 | Lenti ORF clone of Human prickle homolog 2 (Drosophila) (PRICKLE2), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC219763L4 | Lenti ORF clone of Human prickle homolog 2 (Drosophila) (PRICKLE2), mGFP tagged |
CNY 9,030.00 |
|
| RG219763 | PRICKLE2 (tGFP-tagged) - Human prickle homolog 2 (Drosophila) (PRICKLE2) |
CNY 7,784.00 |
|
| SC307799 | PRICKLE2 (untagged)-Human prickle homolog 2 (Drosophila) (PRICKLE2) |
CNY 7,220.00 |
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