United States
Germany
Japan
United Kingdom
China
NF-kB p65 (RELA) (NM_021975) Human Tagged ORF Clone
CAT＃: RC220780
RELA (Myc-DDK-tagged)-Human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1
ORF Plasmid: tGFP
"NM_021975" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 4,104.00
CNY 4,560.00
| Cited in 11 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CMCU; NFKB3; p65 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220780 representing NM_021975
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAACTGTTCCCCCTCATCTTCCCGGCAGAGCCAGCCCAGGCCTCTGGCCCCTATGTGGAGATCA TTGAGCAGCCCAAGCAGCGGGGCATGCGCTTCCGCTACAAGTGCGAGGGGCGCTCCGCGGGCAGCATCCC AGGCGAGAGGAGCACAGATACCACCAAGACCCACCCCACCATCAAGATCAATGGCTACACAGGACCAGGG ACAGTGCGCATCTCCCTGGTCACCAAGGACCCTCCTCACCGGCCTCACCCCCACGAGCTTGTAGGAAAGG ACTGCCGGGATGGCTTCTATGAGGCTGAGCTCTGCCCGGACCGCTGCATCCACAGTTTCCAGAACCTGGG AATCCAGTGTGTGAAGAAGCGGGACCTGGAGCAGGCTATCAGTCAGCGCATCCAGACCAACAACAACCCC TTCCAAGTTCCTATAGAAGAGCAGCGTGGGGACTACGACCTGAATGCTGTGCGGCTCTGCTTCCAGGTGA CAGTGCGGGACCCATCAGGCAGGCCCCTCCGCCTGCCGCCTGTCCTTTCTCATCCCATCTTTGACAATCG TGCCCCCAACACTGCCGAGCTCAAGATCTGCCGAGTGAACCGAAACTCTGGCAGCTGCCTCGGTGGGGAT GAGATCTTCCTACTGTGTGACAAGGTGCAGAAAGAGGACATTGAGGTGTATTTCACGGGACCAGGCTGGG AGGCCCGAGGCTCCTTTTCGCAAGCTGATGTGCACCGACAAGTGGCCATTGTGTTCCGGACCCCTCCCTA CGCAGACCCCAGCCTGCAGGCTCCTGTGCGTGTCTCCATGCAGCTGCGGCGGCCTTCCGACCGGGAGCTC AGTGAGCCCATGGAATTCCAGTACCTGCCAGATACAGACGATCGTCACCGGATTGAGGAGAAACGTAAAA GGACATATGAGACCTTCAAGAGCATCATGAAGAAGAGTCCTTTCAGCGGACCCACCGACCCCCGGCCTCC ACCTCGACGCATTGCTGTGCCTTCCCGCAGCTCAGCTTCTGTCCCCAAGCCAGCACCCCAGCCCTATCCC TTTACGTCATCCCTGAGCACCATCAACTATGATGAGTTTCCCACCATGGTGTTTCCTTCTGGGCAGATCA GCCAGGCCTCGGCCTTGGCCCCGGCCCCTCCCCAAGTCCTGCCCCAGGCTCCAGCCCCTGCCCCTGCTCC AGCCATGGTATCAGCTCTGGCCCAGGCCCCAGCCCCTGTCCCAGTCCTAGCCCCAGGCCCTCCTCAGGCT GTGGCCCCACCTGCCCCCAAGCCCACCCAGGCTGGGGAAGGAACGCTGTCAGAGGCCCTGCTGCAGCTGC AGTTTGATGATGAAGACCTGGGGGCCTTGCTTGGCAACAGCACAGACCCAGCTGTGTTCACAGACCTGGC ATCCGTCGACAACTCCGAGTTTCAGCAGCTGCTGAACCAGGGCATACCTGTGGCCCCCCACACAACTGAG CCCATGCTGATGGAGTACCCTGAGGCTATAACTCGCCTAGTGACAGGGGCCCAGAGGCCCCCCGACCCAG CTCCTGCTCCACTGGGGGCCCCGGGGCTCCCCAATGGCCTCCTTTCAGGAGATGAAGACTTCTCCTCCAT TGCGGACATGGACTTCTCAGCCCTGCTGAGTCAGATCAGCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220780 representing NM_021975
Red=Cloning site Green=Tags(s) MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPG TVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNP FQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGD EIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDREL SEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYP FTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQA VAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTE PMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021975 |
| ORF Size | 1653 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021975.4 |
| RefSeq Size | 1760 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 5970 |
| UniProt ID | Q04206 |
| Domains | RHD, IPT |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway |
| MW | 60 kDa |
| Gene Summary | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
Citations (11)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
microRNA-421-3p prevents inflammatory response in cerebral ischemia/reperfusion injury through targeting m6A Reader YTHDF1 to inhibit p65 mRNA translation
,Zheng, L;Tang, X;Lu, M;Sun, S;Xie, S;Cai, J;Zan, J;,
International Immunopharmacology
,PubMed ID 32890792
[NF-kB p65]
|
|
Long noncoding RNA NKILA regulates HIV-1 replication and latency through repressing NF-ĸB signaling
,Wang, H;Liu, Y;Huan, C;Yang, J;Li, Z;Zheng, B;Wang, Y;Zhang, W;,
J. Virol.
,PubMed ID 32581100
[NF-kB p65]
|
|
Hepatocyte CREBH deficiency aggravates inflammatory liver injury following chemokine-dependent neutrophil infiltration through upregulation of NF-κB p65 in mice
,Noh, JR;Kim, JH;Na, SY;Lee, IB;Seo, YJ;Choi, JH;Seo, Y;Lee, TG;Choi, HS;Kim, YH;Lee, CH;,
Arch. Toxicol.
,PubMed ID 31797000
[NF-kB p65]
|
|
Sevoflurane exerts protective effects on liver ischemia/reperfusion injury by regulating NFKB3 expression via miR-9-5p
,Liao, X;Zhou, S;Zong, J;Wang, Z;,
Exp Ther Med
,PubMed ID 30906455
[NF-kB p65]
|
|
Star-related lipid transfer protein 10 (STARD10): a novel key player in alcohol-induced breast cancer progression
,Floris, A;Luo, J;Frank, J;Zhou, J;Orrù, S;Biancolella, M;Pucci, S;Orlandi, A;Campagna, P;Balzano, A;Ramani, K;Tomasi, ML;,
J. Exp. Clin. Cancer Res.
,PubMed ID 30611309
[NF-kB p65]
|
|
ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum
,Bin, L;Li, X;Richers, B;Streib, JE;Hu, JW;Taylor, P;Leung, DYM;,
J. Allergy Clin. Immunol.
,PubMed ID 29371118
[NF-kB p65]
|
|
Downregulation of miR-16 via URGCP pathway contributes to glioma growth
,Hong, L;Qing, O;Ji, Z;Chengqu, Z;Ying, C;Hao, C;Minhui, X;Lunshan, X;,
Sci Rep
,PubMed ID 29044221
[NF-kB p65]
|
|
MiR-138 indirectly regulates the MDR1 promoter by NF-?B/p65 silencing
,Requenez-Contreras, JL;López-Castillejos, ES;Hernández-Flores, R;Moreno-Eutimio, MA;Granados-Riveron, JT;Martinez-Ruiz, GU;Aquino-Jarquin, G;,
Biochem. Biophys. Res. Commun
,PubMed ID 28153721
[NF-kB p65]
|
|
Oxidized low-density lipoprotein upregulates microRNA-146a via JNK and NF-?B signaling
,Li, Z;Wang, S;Zhao, W;Sun, Z;,
Molecular Medicine Reports
,PubMed ID 26718614
[NF-kB p65]
|
|
CSN-associated USP48 confers stability to nuclear NF-?B/RelA by trimming K48-linked Ub-chains
,Schweitzer, K;Naumann, M;,
Biochim. Biophys. Acta
,PubMed ID 25486460
[NF-kB p65]
|
|
NF-?B p65 repression by the sesquiterpene lactone, Helenalin, contributes to the induction of autophagy cell death
,null,
BMC Complementary and Alternative Medicine
,PubMed ID 22784363
[NF-kB p65]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220780L1 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1, Myc-DDK-tagged |
CNY 6,504.00 |
|
| RC220780L2 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1, mGFP tagged |
CNY 6,504.00 |
|
| RC220780L3 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1, Myc-DDK-tagged |
CNY 6,504.00 |
|
| RC220780L4 | Lenti ORF clone of Human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1, mGFP tagged |
CNY 6,504.00 |
|
| RG220780 | RELA (tGFP-tagged) - Human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1 |
CNY 5,704.00 |
|
| SC313967 | RELA (untagged)-Human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1 |
CNY 6,168.00 |
其它NF-kB p65产品
