Heme oxygenase 2 (HMOX2) (NM_001127204) Human Tagged ORF Clone
CAT#: RC225451
HMOX2 (Myc-DDK-tagged)-Human heme oxygenase (decycling) 2 (HMOX2), transcript variant 1
ORF Plasmid: tGFP
"NM_001127204" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HO-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225451 representing NM_001127204
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCGGAAGTGGAAACCTCAGAGGGGGTAGACGAGTCAGAAAAAAAGAACTCTGGGGCCCTAGAAA AGGAGAACCAAATGAGAATGGCTGACCTCTCGGAGCTCCTGAAGGAAGGGACCAAGGAAGCACACGACCG GGCAGAAAACACCCAGTTTGTCAAGGACTTCTTGAAAGGCAACATTAAGAAGGAGCTGTTTAAGCTGGCC ACCACGGCACTTTACTTCACATACTCAGCCCTCGAGGAGGAAATGGAGCGCAACAAGGACCATCCAGCCT TTGCCCCTTTGTACTTCCCCATGGAGCTGCACCGGAAGGAGGCGCTGACCAAGGACATGGAGTATTTCTT TGGTGAAAACTGGGAGGAGCAGGTGCAGTGCCCCAAGGCTGCCCAGAAGTACGTGGAGCGGATCCACTAC ATAGGGCAGAACGAGCCGGAGCTACTGGTGGCCCATGCATACACCCGCTACATGGGGGATCTCTCGGGGG GCCAGGTGCTGAAGAAGGTGGCCCAGCGAGCACTGAAACTCCCCAGCACAGGGGAAGGGACCCAGTTCTA CCTGTTTGAGAATGTGGACAATGCCCAGCAGTTCAAGCAGCTCTACCGGGCCAGGATGAACGCCCTGGAC CTGAACATGAAGACCAAAGAGAGGATCGTGGAGGAGGCCAACAAGGCTTTTGAGTATAACATGCAGATAT TCAATGAACTGGACCAGGCCGGCTCCACACTGGCCAGAGAGACCTTGGAGGATGGGTTCCCTGTACACGA TGGGAAAGGAGACATGCGTAAATGCCCTTTCTACGCTGCTGAACAAGACAAAGGTGCCCTGGAGGGCAGC AGCTGTCCCTTCCGAACAGCTATGGCTGTGCTGAGGAAGCCCAGCCTCCAGTTCATCCTGGCCGCTGGTG TGGCCCTAGCTGCTGGACTCTTGGCCTGGTACTACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225451 representing NM_001127204
Red=Cloning site Green=Tags(s) MSAEVETSEGVDESEKKNSGALEKENQMRMADLSELLKEGTKEAHDRAENTQFVKDFLKGNIKKELFKLA TTALYFTYSALEEEMERNKDHPAFAPLYFPMELHRKEALTKDMEYFFGENWEEQVQCPKAAQKYVERIHY IGQNEPELLVAHAYTRYMGDLSGGQVLKKVAQRALKLPSTGEGTQFYLFENVDNAQQFKQLYRARMNALD LNMKTKERIVEEANKAFEYNMQIFNELDQAGSTLARETLEDGFPVHDGKGDMRKCPFYAAEQDKGALEGS SCPFRTAMAVLRKPSLQFILAAGVALAAGLLAWYYM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001127204 |
ORF Size | 948 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001127204.1, NP_001120676.1 |
RefSeq ORF | 951 bp |
Locus ID | 3163 |
UniProt ID | P30519 |
Protein Families | Transmembrane |
Protein Pathways | Porphyrin and chlorophyll metabolism |
MW | 35.9 kDa |
Gene Summary | Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225451L3 | Lenti-ORF clone of HMOX2 (Myc-DDK-tagged)-Human heme oxygenase (decycling) 2 (HMOX2), transcript variant 1 |
CNY 5,890.00 |
|
RC225451L4 | Lenti-ORF clone of HMOX2 (mGFP-tagged)-Human heme oxygenase (decycling) 2 (HMOX2), transcript variant 1 |
CNY 5,890.00 |
|
RG225451 | HMOX2 (tGFP-tagged) - Human heme oxygenase (decycling) 2 (HMOX2), transcript variant 1 |
CNY 4,370.00 |
|
SC322968 | HMOX2 (untagged)-Human heme oxygenase (decycling) 2 (HMOX2), transcript variant 1 |
CNY 6,270.00 |
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