Ankyrin brain (ANK2) (NM_001127493) Human Tagged ORF Clone
CAT#: RC226435
- TrueORF®
ANK2 (Myc-DDK-tagged)-Human ankyrin 2, neuronal (ANK2), transcript variant 3
ORF Plasmid: tGFP
"NM_001127493" in other vectors (2)
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CNY 18,620.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ANK-2; brank-2; CFAP87; FAP87; LQT4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC226435 representing NM_001127493
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACCATGTTGCAAAAGTCTGACAGCAATGCAAGCTTCCTCCGTGCTGCCAGAGCAGGCAACCTGG ACAAAGTTGTGGAATATCTGAAGGGGGGCATAGACATCAATACCTGCAATCAGAATGGACTCAACGCTCT CCATCTGGCTGCCAAGGAAGGCCACGTGGGGCTGGTGCAGGAGCTGCTGGGAAGAGGGTCCTCTGTGGAT TCTGCCACTAAGAAGGGAAATACCGCTCTTCACATTGCATCTTTGGCTGGACAAGCAGAAGTTGTCAAAG TTCTTGTTAAGGAAGGAGCCAATATTAATGCACAGTCTCAGAATGGCTTTACTCCTTTATACATGGCTGC CCAAGAGAATCACATTGATGTTGTAAAATATTTGCTGGAAAATGGAGCTAATCAGAGCACTGCTACAGAG GATGGCTTTACTCCTCTAGCTGTGGCACTCCAGCAAGGACACAACCAGGCGGTGGCCATCCTCTTGGAGA ATGACACCAAAGGGAAAGTGAGGCTGCCAGCTCTGCATATTGCCGCTAGGAAAGACGACACCAAATCTGC CGCACTTCTGCTTCAGAATGACCACAATGCTGACGTACAATCCAAGATGATGGTGAATAGGACAACTGAG AGTGGTTTTACCCCTTTGCACATAGCTGCACATTACGGAAATGTCAACGTGGCAACTCTTCTTCTAAACC GGGGAGCTGCTGTGGACTTCACAGCCAGGAATGGAATCACTCCTCTGCATGTGGCTTCCAAAAGAGGAAA TACAAACATGGTGAAGCTCTTACTGGATCGAGGCGGTCAGATCGATGCCAAAACTAGGGATGGGTTGACA CCACTTCACTGTGCTGCACGAAGTGGGCATGACCAAGTGGTGGAACTTCTGTTGGAACGGGGTGCCCCCT TGCTGGCAAGGACTAAGAATGGGCTGTCTCCACTACACATGGCTGCCCAGGGAGACCACGTGGAATGTGT GAAGCACCTGTTACAGCACAAGGCACCTGTTGATGATGTCACCCTAGACTACCTGACAGCCCTCCACGTT GCTGCGCACTGTGGCCACTACCGTGTAACCAAACTCCTTTTAGACAAGAGAGCCAATCCGAACGCCAGAG CCCTGAATGGTTTTACTCCACTGCACATTGCCTGCAAGAAAAACCGCATCAAAGTCATGGAACTGCTGGT GAAATATGGGGCTTCAATCCAAGCTATAACAGAGTCTGGCCTCACACCAATACATGTGGCTGCCTTCATG GGCCACTTGAACATTGTCCTCCTTCTGCTGCAGAACGGAGCCTCTCCAGATGTCACTAACATTCGTGGTG AGACGGCACTACACATGGCAGCCCGAGCCGGGCAGGTGGAAGTGGTCCGATGCCTCCTGAGAAATGGTGC CCTTGTTGATGCCAGAGCCAGGGAGGAACAGACACCTTTACATATTGCCTCCCGCCTGGGTAAGACAGAA ATTGTCCAGCTGCTTCTACAACATATGGCTCATCCAGATGCGGCCACTACAAATGGGTACACACCACTGC ACATCTCTGCCCGGGAGGGCCAGGTGGATGTGGCATCAGTCCTATTGGAAGCAGGAGCAGCCCACTCCTT AGCTACCAAGAAGGGTTTTACTCCCCTGCATGTAGCAGCCAAGTATGGAAGCCTGGATGTGGCAAAACTT CTCTTGCAACGCCGTGCTGCCGCAGATTCTGCAGGGAAGAACGGCCTTACCCCGCTCCATGTTGCTGCTC ATTATGACAACCAGAAGGTGGCGCTGCTGTTACTGGAGAAGGGTGCTTCCCCTCATGCCACTGCCAAGAA TGGCTATACTCCGTTACATATTGCTGCCAAGAAGAATCAAATGCAGATAGCTTCCACACTCCTGAACTAT GGAGCAGAGACAAACATTGTGACAAAGCAAGGAGTAACTCCACTCCATCTGGCCTCGCAGGAGGGGCACA CAGATATGGTTACCTTGCTTCTGGATAAGGGAGCCAATATCCACATGTCAACTAAGAGTGGACTCACATC CTTACACCTTGCAGCCCAGGAAGATAAAGTGAATGTTGCTGATATTCTCACCAAGCATGGAGCTGATCAG GATGCTCATACAAAGCTTGGTTACACACCTTTAATTGTGGCCTGTCACTATGGAAATGTGAAAATGGTCA ACTTTCTTCTGAAGCAGGGAGCAAATGTTAACGCAAAAACCAAGAACGGCTACACGCCTTTGCACCAGGC CGCTCAGCAGGGTCACACGCACATCATCAACGTCCTGCTCCAGCATGGGGCCAAGCCCAACGCCACCACT GCGAATGGCAACACTGCCTTGGCGATTGCTAAGCGTCTGGGCTACATCTCCGTGGTCGACACCCTGAAGG TTGTGACTGAGGAGGTCACCACCACCACCACAACTATTACAGAAAAACACAAACTAAATGTACCTGAGAC GATGACTGAGGTTCTTGATGTTTCTGATGAAGAGGGTGATGACACAATGACTGGTGATGGGGGAGAATAC CTTAGGCCTGAGGACCTAAAAGAACTGGGTGATGACTCACTACCCAGCAGTCAGTTCCTGGATGGTATGA ATTACCTGCGATACAGCTTGGAGGGAGGACGATCTGACAGCCTTCGATCCTTCAGTTCCGACAGGTCTCA CACTCTGAGCCATGCCTCCTACCTGAGGGACAGTGCCGTGATGGATGACTCAGTTGTGATTCCCAGTCAC CAGGTGTCAACTCTAGCCAAGGAGGCAGAAAGGAATTCTTATCGCCTAAGCTGGGGCACTGAGAACTTAG ACAACGTGGCTCTTTCTTCTAGTCCTATTCATTCAGGCCGCGCCTCTCCATGTCTTGAACGTGACAACAG CAGTTTCCTGGTTAGTTTTATGGTGGATGCCCGAGGTGGTGCTATGCGAGGATGCAGACACAATGGGCTC CGAATCATTATTCCACCTCGGAAATGTACTGCTCCAACGCGAGTCACCTGCCGACTGGTCAAGCGCCACA GACTGGCAACAATGCCTCCAATGGTGGAAGGAGAAGGCCTGGCCAGTCGCCTGATCGAAGTTGGACCTTC TGGTGCTCAGTTCCTTGGTAAACTTCACCTGCCAACGGCTCCTCCCCCACTTAATGAGGGAGAAAGTTTG GTCAGCCGCATTCTTCAGCTGGGGCCTCCTGGAACCAAATTCCTTGGGCCTGTGATCGTGGAGATCCCTC ACTTTGCGGCCCTTCGAGGAAAGGAAAGGGAACTGGTGGTCCTGCGCAGTGAGAATGGGGACAGCTGGAA AGAGCATTTCTGTGACTACACTGAAGATGAATTGAATGAAATTCTTAACGGCATGGATGAAGTACTGGAT AGCCCAGAAGACCTAGAAAAGAAACGAATCTGCCGCATCATCACCCGAGACTTCCCACAGTACTTTGCAG TGGTGTCTCGTATCAAACAGGACAGCAATCTGATTGGCCCAGAAGGAGGTGTACTGAGCAGCACAGTGGT GCCCCAGGTGCAGGCCGTCTTCCCAGAGGGGGCACTCACCAAGCGGATCCGCGTAGGCCTGCAGGCTCAA CCTATGCACAGTGAGCTGGTTAAGAAGATCCTAGGCAACAAAGCTACCTTCAGCCCTATAGTCACTTTGG AACCTAGAAGAAGAAAATTCCACAAACCAATTACCATGACCATTCCTGTCCCCAAAGCTTCAAGTGATGT CATGTTGAATGGTTTTGGGGGAGATGCACCAACCTTAAGATTACTATGCAGCATAACAGGTGGAACCACC CCTGCCCAGTGGGAAGATATTACAGGAACTACGCCATTAACATTTGTCAATGAATGTGTTTCCTTTACAA CAAACGTGTCTGCCAGGTTCTGGCTGATAGATTGTCGACAGATCCAGGAATCCGTTACTTTTGCATCACA AGTATACAGAGAAATTATCTGCGTACCTTATATGGCCAAATTTGTAGTGTTTGCCAAATCACATGACCCC ATTGAAGCCAGGTTGAGGTGTTTCTGCATGACTGATGATAAAGTGGATAAGACCCTTGAACAACAAGAAA ATTTTGCTGAGGTGGCCAGAAGCAGGGATGTGGAGGTGTTAGAAGGAAAACCCATCTACGTTGATTGTTT CGGCAACTTGGTACCATTAACTAAAAGTGGCCAGCATCATATATTCAGTTTTTTTGCCTTCAAAGAAAAT AGACTTCCTCTATTTGTCAAGGTACGCGATACGACTCAGGAACCTTGCGGACGACTATCATTTATGAAGG AGCCAAAATCCACGAGAGGCCTGGTGCATCAAGCTATTTGCAACTTAAACATCACTTTGCCGATTTATAC AAAGGAATCAGAGTCAGATCAAGAACAGGAGGAAGAGATCGATATGACATCAGAAAAAAATCCACAGGAT GAGCAGGAACGGATCGAGGAAAGGCTGGCTTATATTGCTGATCACCTTGGCTTCAGCTGGACAGAATTAG CAAGAGAACTGGATTTCACTGAGGAGCAAATTCATCAAATTCGAATTGAAAATCCCAACTCTCTTCAAGA CCAGAGTCATGCACTGTTGAAGTACTGGCTAGAGAGGGATGGGAAACATGCTACAGATACCAACCTCGTT GAATGTCTCACCAAGATCAACCGAATGGATATTGTTCATCTCATGGAGACCAACACAGAACCTCTCCAGG AGCGCATCAGTCATAGTTATGCAGAAATTGAACAGACCATTACACTGGATCATAGTGAAGGGTTCTCGGT ACTTCAAGAGGAGTTATGCACTGCACAGCACAAGCAGAAAGAGGAGCAAGCTGTTTCTAAAGAAAGTGAG ACCTGCGATCACCCTCCTATCGTCTCAGAGGAAGACATTTCTGTTGGTTATTCCACTTTTCAGGATGGCG TCCCCAAAACTGAGGGGGACAGCTCAGCAACAGCACTCTTTCCCCAAACTCACAAGGAGCAAGTTCAACA GGATTTCTCAGGGAAAATGCAAGACCTGCCTGAAGAGTCATCTCTGGAATATCAGCAGGAATATTTTGTG ACAACTCCAGGAACAGAAACATCAGAGACTCAGAAGGCTATGATAGTACCCAGCTCTCCCAGCAAGACAC CTGAGGAAGTTAGCACCCCTGCAGAGGAGGAGAAGCTGTACCTCCAGACCCCAACATCCAGCGAGCGGGG AGGCTCTCCCATCATACAAGAACCCGAAGAGCCCTCAGAGCACAGAGAGGAGAGCTCTCCGCGGAAAACC AGCCTCGTAATAGTGGAGTCTGCCGATAACCAGCCTGAGACCTGTGAAAGACTCGATGAAGATGCAGCTT TTGAAAAGGGAGACGATATGCCTGAAATACCCCCAGAAACAGTCACAGAAGAAGAATACATTGATGAGCA TGGACACACCGTGGTAAAGAAGGTTACTAGGAAAATCATTAGGCGGTATGTATCCTCTGAAGGCACAGAG AAAGAAGAGATTATGGTGCAGGGAATGCCACAGGAACCTGTCAACATCGAGGAAGGGGATGGCTATTCCA AAGTTATAAAGCGTGTTGTATTGAAGAGTGACACCGAGCAGTCAGAGGACAACAATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226435 representing NM_001127493
Red=Cloning site Green=Tags(s) MTTMLQKSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVD SATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATE DGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTE SGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLT PLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHV AAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFM GHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTE IVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKL LLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNY GAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQ DAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATT ANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEY LRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAVMDDSVVIPSH QVSTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRASPCLERDNSSFLVSFMVDARGGAMRGCRHNGL RIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGKLHLPTAPPPLNEGESL VSRILQLGPPGTKFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHFCDYTEDELNEILNGMDEVLD SPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQ PMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTT PAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDP IEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKEN RLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNPQD EQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTNLV ECLTKINRMDIVHLMETNTEPLQERISHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEQAVSKESE TCDHPPIVSEEDISVGYSTFQDGVPKTEGDSSATALFPQTHKEQVQQDFSGKMQDLPEESSLEYQQEYFV TTPGTETSETQKAMIVPSSPSKTPEEVSTPAEEEKLYLQTPTSSERGGSPIIQEPEEPSEHREESSPRKT SLVIVESADNQPETCERLDEDAAFEKGDDMPEIPPETVTEEEYIDEHGHTVVKKVTRKIIRRYVSSEGTE KEEIMVQGMPQEPVNIEEGDGYSKVIKRVVLKSDTEQSEDNNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001127493 |
ORF Size | 5589 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001127493.2 |
RefSeq Size | 8082 bp |
RefSeq ORF | 5592 bp |
Locus ID | 287 |
UniProt ID | Q01484 |
Protein Families | Druggable Genome |
MW | 204.8 kDa |
Gene Summary | This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Dec 2011] |
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