BCAR3 (NM_001261408) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AND-34; MIG7; NSP2; SH2D3B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234936 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGGAAAATTTGCAAGCCTTCCCAGAAACATGCCGGTGAATCACCAGTTCCCCCTGGCCTCAT CCATGGACCTTCTGAGCAGCAGGTCCCCTCTCGCTGAGCATCGCCCAGATGCCTATCAAGATGTGTCTAT ACATGGCACCCTTCCACGGAAGAAAAAAGGTCCTCCTCCCATAAGGTCCTGTGATGACTTCAGTCACATG GGCACCCTCCCCCACTCCAAATCCCCACGGCAGAACTCGCCTGTGACCCAGGATGGCATCCAGGAGAGCC CATGGCAGGACCGGCACGGCGAAACCTTCACCTTCAGGGATCCACATCTTCTGGACCCAACTGTGGAATA TGTGAAGTTCTCCAAGGAGAGGCACATCATGGACAGGACCCCCGAGAAACTGAAGAAGGAGCTGGAGGAG GAGCTGCTCCTGAGCAGCGAGGACCTGCGCAGCCATGCCTGGTACCACGGCCGCATCCCCCGACAGGTGT CTGAAAACCTTGTGCAGCGAGATGGTGACTTCCTAGTTCGTGACTCTCTGTCCAGCCCTGGGAACTTTGT CCTGACCTGTCAGTGGAAGAACCTCGCTCAGCACTTCAAAATCAACCGGACAGTTCTGCGACTCAGCGAG GCCTACAGCCGCGTGCAGTACCAGTTCGAGATGGAGAGCTTCGACTCCATCCCCGGCCTGGTGCGCTGCT ACGTGGGCAACCGCCGGCCCATCTCCCAGCAGAGTGGCGCCATCATCTTCCAGCCCATCAACAGGACGGT GCCTCTGCGGTGCCTGGAGGAGCATTATGGCACCTCCCCAGGCCAGGCCCGGGAGGGCAGCCTCACCAAG GGAAGGCCGGATGTGGCCAAGAGGCTGAGCCTCACCATGGGTGGCGTCCAGGCCCGAGAGCAGAATTTGC CCAGGGGAAACCTCCTCAGAAACAAAGAAAAGAGTGGTAGCCAGCCCGCCTGCCTGGATCACATGCAGGA CAGAAGAGCCTTGTCCCTCAAAGCCCACCAGTCAGAGAGCTACCTGCCGATTGGCTGCAAGCTGCCACCT CAGTCCTCGGGTGTGGACACAAGCCCCTGCCCAAACTCACCTGTGTTCAGGACGGGAAGCGAGCCTGCCC TGAGCCCAGCAGTGGTTCGGAGGGTCTCCTCAGACGCCAGGGCTGGGGAGGCGCTGAGGGGATCAGACAG TCAACTGTGCCCTAAGCCCCCGCCTAAGCCCTGCAAGGTGCCGTTCCTCAAGGTTCCCTCGTCTCCCTCT GCCTGGCTCAACTCAGAGGCCAACTACTGTGAACTGAACCCAGCGTTTGCCACAGGCTGCGGCAGGGGAG CAAAGCTACCCTCATGTGCCCAGGGAAGCCACACAGAACTGCTCACAGCCAAGCAGAATGAGGCGCCAGG TCCCCGGAACTCTGGCGTCAACTACTTGATCCTTGATGATGATGACAGGGAAAGACCTTGGGAACCTGCG GCAGCTCAGATGGAGAAGGGGCAGTGGGACAAGGGCGAGTTTGTGACGCCCCTCCTGGAGACTGTCTCCT CCTTCAGGCCCAACGAGTTTGAGTCAAAGTTCCTTCCCCCTGAGAATAAGCCCCTGGAAACAGCAATGTT GAAACGTGCAAAAGAACTGTTCACCAACAACGACCCCAAGGTCATCGCCCAGCACGTACTGAGCATGGAC TGCAGGGTTGCTAGGATACTTGGAGTCTCTGAAGAGATGAGGAGGAACATGGGGGTGAGCTCAGGCCTGG AACTCATTACCTTGCCTCACGGACACCAGCTGCGCCTGGACATAATTGAAAGACACAACACAATGGCCAT CGGCATTGCAGTGGACATTCTGGGATGCACGGGCACTTTGGAGGACCGAGCGGCCACTCTGAGTAAGATC ATCCAGGTGGCGGTGGAACTGAAGGATTCCATGGGGGACCTCTATTCCTTCTCAGCTCTCATGAAAGCCC TGGAAATGCCACAGATCACAAGGTTAGAAAAGACGTGGACTGCTCTGCGGCACCAGTACACCCAAACTGC CATTCTCTATGAGAAACAGCTGAAGCCCTTCAGCAAACTCCTGCATGAAGGCAGAGAGTCCACATGTGTT CCCCCAAACAATGTATCAGTCCCACTGCTGATGCCGCTTGTGACGTTAATGGAGCGCCAGGCTGTGACTT TTGAAGGAACCGACATGTGGGAAAAAAACGACCAGAGCTGTGAAATCATGCTGAACCATTTGGCAACAGC GCGATTCATGGCCGAGGCTGCAGACAGCTACCGGATGAATGCTGAGAGGATCCTGGCAGGTTTTCAACCA GATGAAGAAATGAATGAAATCTGCAAGACTGAATTTCAAATGCGATTGCTATGGGGCAGCAAAGGTGCAC AAGTCAATCAGACAGAGAGATATGAGAAATTCAACCAGATTTTAACTGCCCTCTCGCGTAAATTGGAACC TCCTCCTGTAAAGCAGGCAGAGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234936 protein sequence Red=Cloning site Green=Tags(s) MAAGKFASLPRNMPVNHQFPLASSMDLLSSRSPLAEHRPDAYQDVSIHGTLPRKKKGPPPIRSCDDFSHM GTLPHSKSPRQNSPVTQDGIQESPWQDRHGETFTFRDPHLLDPTVEYVKFSKERHIMDRTPEKLKKELEE ELLLSSEDLRSHAWYHGRIPRQVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSE AYSRVQYQFEMESFDSIPGLVRCYVGNRRPISQQSGAIIFQPINRTVPLRCLEEHYGTSPGQAREGSLTK GRPDVAKRLSLTMGGVQAREQNLPRGNLLRNKEKSGSQPACLDHMQDRRALSLKAHQSESYLPIGCKLPP QSSGVDTSPCPNSPVFRTGSEPALSPAVVRRVSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPSSPS AWLNSEANYCELNPAFATGCGRGAKLPSCAQGSHTELLTAKQNEAPGPRNSGVNYLILDDDDRERPWEPA AAQMEKGQWDKGEFVTPLLETVSSFRPNEFESKFLPPENKPLETAMLKRAKELFTNNDPKVIAQHVLSMD CRVARILGVSEEMRRNMGVSSGLELITLPHGHQLRLDIIERHNTMAIGIAVDILGCTGTLEDRAATLSKI IQVAVELKDSMGDLYSFSALMKALEMPQITRLEKTWTALRHQYTQTAILYEKQLKPFSKLLHEGRESTCV PPNNVSVPLLMPLVTLMERQAVTFEGTDMWEKNDQSCEIMLNHLATARFMAEAADSYRMNAERILAGFQP DEEMNEICKTEFQMRLLWGSKGAQVNQTERYEKFNQILTALSRKLEPPPVKQAEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001261408
ORF Size	2475 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001261408.2
RefSeq Size	3240 bp
RefSeq ORF	2478 bp
Locus ID	8412
UniProt ID	O75815
Protein Families	Druggable Genome
MW	92.6 kDa
Gene Summary	Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

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