Carboxypeptidase D (CPD) (NM_001199775) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GP180
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235181 representing NM_001199775 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTTTGTGCTTTCTGGAAATCTGCATGGTGGCTCAGTGGTAGCAAGCTATCCTTTTGATGATTCTC CAGAACATAAGGCCACTGGAATCTATAGCAAAACCTCAGATGATGAAGTATTTAAATACTTGGCAAAAGC TTATGCTTCAAACCACCCCATAATGAAAACTGGTGAGCCTCATTGTCCAGGAGATGAAGACGAGACTTTC AAAGATGGAATCACAAACGGCGCACATTGGTATGATGTGGAAGGTGGTATGCAAGATTACAATTATGTGT GGGCCAACTGTTTTGAGATCACATTAGAACTGTCTTGTTGCAAGTACCCACCTGCTTCACAGCTTCGACA GGAATGGGAGAACAATCGTGAGTCTTTGATCACATTGATTGAAAAGGTTCACATTGGAGTGAAAGGATTT GTTAAAGATTCCATAACAGGATCTGGGTTAGAGAATGCAACCATCTCAGTGGCTGGTATTAATCATAATA TCACAACAGGCAGATTTGGTGATTTCTACCGATTACTTGTTCCTGGAACTTACAACCTTACAGTAGTTTT AACTGGGTATATGCCATTGACTGTTACTAATGTAGTGGTGAAAGAAGGACCAGCCACAGAGGTGGATTTT TCTCTTAGGCCAACTGTAACTTCAGTAATCCCTGACACGACAGAGGCTGTATCAACTGCTAGCACAGTTG CTATACCTAATATTCTTTCTGGAACATCATCCTCCTACCAGCCAATTCAGCCAAAGGACTTTCACCACCA CCATTTCCCTGATATGGAAATCTTCTTGAGAAGGTTTGCCAATGAATATCCTAACATTACCCGGCTTTAT TCCTTGGGAAAATCAGTAGAGTCAAGAGAACTTTATGTGATGGAGATATCTGATAATCCGGGTGTCCATG AACCAGGTGAACCAGAATTTAAGTACATTGGAAATATGCATGGAAATGAAGTGGTTGGAAGAGAACTGCT GTTGAACCTCATAGAATACCTTTGTAAGAACTTTGGAACAGACCCTGAAGTCACAGATTTGGTTCATAAC ACTAGAATTCACCTTATGCCATCCATGAATCCTGATGGGTATGAAAAGTCCCAGGAAGGAGATTCAATAA GTGTAATTGGCAGAAACAACAGCAACAACTTTGACCTGAACCGAAATTTCCCAGACCAGTTTGTTCAGAT CACAGATCCTACGCAACCAGAAACTATTGCTGTAATGAGCTGGATGAAGTCCTATCCATTTGTACTTTCA GCAAACCTGCATGGAGGTTCTTTGGTGGTTAACTACCCTTTTGATGATGATGAACAAGGACTTGCCACAT ATAGTAAATCACCAGATGATGCTGTGTTCCAACAAATAGCACTTTCTTATTCCAAGGAAAATTCCCAGAT GTTTCAAGGTAGACCTTGCAAGAATATGTATCCTAATGAATATTTTCCTCATGGAATAACAAATGGAGCT AGTTGGTATAATGTGCCAGGAGGAATGCAGGACTGGAACTATTTACAAACAAATTGCTTTGAAGTGACTA TTGAACTAGGTTGTGTGAAATATCCACTTGAGAAAGAGCTGCCAAACTTTTGGGAACAGAATCGAAGATC ACTAATCCAGTTTATGAAACAGGTTCATCAGGGCGTCAGAGGATTTGTTCTAGATGCCACAGATGGCAGG GGTATATTAAATGCCACCATTAGTGTTGCTGAGATTAATCACCCAGTGACTACTTACAAAACTGGAGATT ACTGGCGTCTCTTGGTTCCAGGAACTTATAAAATCACAGCATCTGCTCGAGGGTATAATCCAGTTACCAA GAATGTGACTGTCAAGAGTGAAGGCGCTATTCAGGTCAACTTCACACTTGTTCGATCCTCAACAGATTCA AACAATGAATCAAAGAAAGGAAAAGGGGCTAGCAGCAGCACCAATGATGCCAGTGATCCAACTACTAAAG AGTTTGAAACTTTAATTAAAGACCTTTCAGCGGAGAATGGTTTGGAAAGCCTCATGTTACGCTCCTCCTC AAATCTGGCTCTGGCTCTTTATCGATACCATTCCTACAAAGACTTATCAGAGTTTCTGAGAGGACTTGTA ATGAACTATCCACATATTACAAATCTTACCAATTTGGGACAGAGCACTGAATATCGTCACATTTGGTCCC TTGAAATCTCCAATAAGCCCAATGTATCTGAGCCTGAAGAACCAAAGATTCGTTTTGTTGCTGGTATCCA TGGAAATGCGCCAGTTGGAACTGAACTGCTTTTGGCTCTGGCAGAATTTCTCTGCCTGAACTACAAAAAG AACCCAGCTGTTACCCAATTGGTTGACAGGACTAGGATTGTGATTGTCCCTTCTCTAAATCCAGATGGGC GAGAGAGAGCTCAAGAGAAAGACTGTACTTCAAAAATAGGACAAACAAATGCTCGTGGCAAAGATTTGGA TACAGACTTCACAAATAATGCCTCCCAACCTGAGACCAAAGCCATCATTGAAAATTTGATTCAAAAACAG GACTTTAGTCTTTCTGTTGCCTTAGATGGTGGTTCCATGCTGGTCACATATCCTTATGACAAGCCAGTAC AGACAGTGGAAAATAAAGAGACTCTGAAGCATTTGGCATCTCTTTATGCAAATAATCATCCATCCATGCA CATGGGTCAGCCCAGTTGCCCAAATAAATCAGATGAGAATATTCCAGGAGGAGTAATGCGTGGAGCAGAA TGGCATAGTCACCTGGGCAGCATGAAGGATTATAGTGTCACCTATGGCCATTGTCCGGAAATCACAGTAT ACACAAGCTGCTGTTACTTTCCTAGTGCTGCACGACTCCCTTCCTTGTGGGCAGACAATAAGAGATCTCT TCTTAGTATGTTAGTGGAGGTTCACAAGGGAGTTCATGGATTTGTTAAAGATAAGACTGGAAAGCCAATC TCTAAAGCAGTCATTGTACTTAATGAAGGAATAAAGGTACAAACAAAAGAGGGAGGTTATTTCCATGTAC TCTTAGCGCCAGGTGTCCATAACATTATTGCCATCGCTGATGGGTACCAGCAACAACATTCACAGGTCTT TGTGCATCATGATGCAGCTAGTTCTGTGGTGATAGTCTTTGACACAGATAACCGGATATTTGGTTTGCCA AGGGAGCTTGTGGTAACTGTATCAGGTGCTACTATGTCGGCATTGATCCTAACAGCTTGCATTATTTGGT GCATCTGCTCAATCAAGTCTAATAGACACAAGGATGGCTTTCATCGGCTCAGGCAGCATCATGATGAGTA TGAAGATGAAATTCGCATGATGTCTACCGGCTCCAAGAAGTCCCTCCTAAGCCATGAGTTCCAGGATGAA ACAGACACTGAAGAGGAAACATTATATTCTAGCAAACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235181 representing NM_001199775 Red=Cloning site Green=Tags(s) MRFVLSGNLHGGSVVASYPFDDSPEHKATGIYSKTSDDEVFKYLAKAYASNHPIMKTGEPHCPGDEDETF KDGITNGAHWYDVEGGMQDYNYVWANCFEITLELSCCKYPPASQLRQEWENNRESLITLIEKVHIGVKGF VKDSITGSGLENATISVAGINHNITTGRFGDFYRLLVPGTYNLTVVLTGYMPLTVTNVVVKEGPATEVDF SLRPTVTSVIPDTTEAVSTASTVAIPNILSGTSSSYQPIQPKDFHHHHFPDMEIFLRRFANEYPNITRLY SLGKSVESRELYVMEISDNPGVHEPGEPEFKYIGNMHGNEVVGRELLLNLIEYLCKNFGTDPEVTDLVHN TRIHLMPSMNPDGYEKSQEGDSISVIGRNNSNNFDLNRNFPDQFVQITDPTQPETIAVMSWMKSYPFVLS ANLHGGSLVVNYPFDDDEQGLATYSKSPDDAVFQQIALSYSKENSQMFQGRPCKNMYPNEYFPHGITNGA SWYNVPGGMQDWNYLQTNCFEVTIELGCVKYPLEKELPNFWEQNRRSLIQFMKQVHQGVRGFVLDATDGR GILNATISVAEINHPVTTYKTGDYWRLLVPGTYKITASARGYNPVTKNVTVKSEGAIQVNFTLVRSSTDS NNESKKGKGASSSTNDASDPTTKEFETLIKDLSAENGLESLMLRSSSNLALALYRYHSYKDLSEFLRGLV MNYPHITNLTNLGQSTEYRHIWSLEISNKPNVSEPEEPKIRFVAGIHGNAPVGTELLLALAEFLCLNYKK NPAVTQLVDRTRIVIVPSLNPDGRERAQEKDCTSKIGQTNARGKDLDTDFTNNASQPETKAIIENLIQKQ DFSLSVALDGGSMLVTYPYDKPVQTVENKETLKHLASLYANNHPSMHMGQPSCPNKSDENIPGGVMRGAE WHSHLGSMKDYSVTYGHCPEITVYTSCCYFPSAARLPSLWADNKRSLLSMLVEVHKGVHGFVKDKTGKPI SKAVIVLNEGIKVQTKEGGYFHVLLAPGVHNIIAIADGYQQQHSQVFVHHDAASSVVIVFDTDNRIFGLP RELVVTVSGATMSALILTACIIWCICSIKSNRHKDGFHRLRQHHDEYEDEIRMMSTGSKKSLLSHEFQDE TDTEEETLYSSKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199775
ORF Size	3399 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199775.1, NP_001186704.1
RefSeq Size	8422 bp
RefSeq ORF	3402 bp
Locus ID	1362
UniProt ID	O75976
Protein Families	Druggable Genome, Protease, Transmembrane
MW	126.9 kDa
Gene Summary	The metallocarboxypeptidase family of enzymes is divided into 2 subfamilies based on sequence similarities. The pancreatic carboxypeptidase-like and the regulatory B-type carboxypeptidase subfamilies. Carboxypeptidase D has been identified as a regulatory B-type carboxypeptidase. CPD is a homolog of duck gp180, a hepatitis B virus-binding protein. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Jul 2008]

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