Carboxypeptidase D (CPD) (NM_001199775) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GP180
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331360 representing NM_001199775. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGGTTTGTGCTTTCTGGAAATCTGCATGGTGGCTCAGTGGTAGCAAGCTATCCTTTTGATGATTCT CCAGAACATAAGGCCACTGGAATCTATAGCAAAACCTCAGATGATGAAGTATTTAAATACTTGGCAAAA GCTTATGCTTCAAACCACCCCATAATGAAAACTGGTGAGCCTCATTGTCCAGGAGATGAAGACGAGACT TTCAAAGATGGAATCACAAACGGCGCACATTGGTATGATGTGGAAGGTGGTATGCAAGATTACAATTAT GTGTGGGCCAACTGTTTTGAGATCACATTAGAACTGTCTTGTTGCAAGTACCCACCTGCTTCACAGCTT CGACAGGAATGGGAGAACAATCGTGAGTCTTTGATCACATTGATTGAAAAGGTTCACATTGGAGTGAAA GGATTTGTTAAAGATTCCATAACAGGATCTGGGTTAGAGAATGCAACCATCTCAGTGGCTGGTATTAAT CATAATATCACAACAGGCAGATTTGGTGATTTCTACCGATTACTTGTTCCTGGAACTTACAACCTTACA GTAGTTTTAACTGGGTATATGCCATTGACTGTTACTAATGTAGTGGTGAAAGAAGGACCAGCCACAGAG GTGGATTTTTCTCTTAGGCCAACTGTAACTTCAGTAATCCCTGACACGACAGAGGCTGTATCAACTGCT AGCACAGTTGCTATACCTAATATTCTTTCTGGAACATCATCCTCCTACCAGCCAATTCAGCCAAAGGAC TTTCACCACCACCATTTCCCTGATATGGAAATCTTCTTGAGAAGGTTTGCCAATGAATATCCTAACATT ACCCGGCTTTATTCCTTGGGAAAATCAGTAGAGTCAAGAGAACTTTATGTGATGGAGATATCTGATAAT CCGGGTGTCCATGAACCAGGTGAACCAGAATTTAAGTACATTGGAAATATGCATGGAAATGAAGTGGTT GGAAGAGAACTGCTGTTGAACCTCATAGAATACCTTTGTAAGAACTTTGGAACAGACCCTGAAGTCACA GATTTGGTTCATAACACTAGAATTCACCTTATGCCATCCATGAATCCTGATGGGTATGAAAAGTCCCAG GAAGGAGATTCAATAAGTGTAATTGGCAGAAACAACAGCAACAACTTTGACCTGAACCGAAATTTCCCA GACCAGTTTGTTCAGATCACAGATCCTACGCAACCAGAAACTATTGCTGTAATGAGCTGGATGAAGTCC TATCCATTTGTACTTTCAGCAAACCTGCATGGAGGTTCTTTGGTGGTTAACTACCCTTTTGATGATGAT GAACAAGGACTTGCCACATATAGTAAATCACCAGATGATGCTGTGTTCCAACAAATAGCACTTTCTTAT TCCAAGGAAAATTCCCAGATGTTTCAAGGTAGACCTTGCAAGAATATGTATCCTAATGAATATTTTCCT CATGGAATAACAAATGGAGCTAGTTGGTATAATGTGCCAGGAGGAATGCAGGACTGGAACTATTTACAA ACAAATTGCTTTGAAGTGACTATTGAACTAGGTTGTGTGAAATATCCACTTGAGAAAGAGCTGCCAAAC TTTTGGGAACAGAATCGAAGATCACTAATCCAGTTTATGAAACAGGTTCATCAGGGCGTCAGAGGATTT GTTCTAGATGCCACAGATGGCAGGGGTATATTAAATGCCACCATTAGTGTTGCTGAGATTAATCACCCA GTGACTACTTACAAAACTGGAGATTACTGGCGTCTCTTGGTTCCAGGAACTTATAAAATCACAGCATCT GCTCGAGGGTATAATCCAGTTACCAAGAATGTGACTGTCAAGAGTGAAGGCGCTATTCAGGTCAACTTC ACACTTGTTCGATCCTCAACAGATTCAAACAATGAATCAAAGAAAGGAAAAGGGGCTAGCAGCAGCACC AATGATGCCAGTGATCCAACTACTAAAGAGTTTGAAACTTTAATTAAAGACCTTTCAGCGGAGAATGGT TTGGAAAGCCTCATGTTACGCTCCTCCTCAAATCTGGCTCTGGCTCTTTATCGATACCATTCCTACAAA GACTTATCAGAGTTTCTGAGAGGACTTGTAATGAACTATCCACATATTACAAATCTTACCAATTTGGGA CAGAGCACTGAATATCGTCACATTTGGTCCCTTGAAATCTCCAATAAGCCCAATGTATCTGAGCCTGAA GAACCAAAGATTCGTTTTGTTGCTGGTATCCATGGAAATGCGCCAGTTGGAACTGAACTGCTTTTGGCT CTGGCAGAATTTCTCTGCCTGAACTACAAAAAGAACCCAGCTGTTACCCAATTGGTTGACAGGACTAGG ATTGTGATTGTCCCTTCTCTAAATCCAGATGGGCGAGAGAGAGCTCAAGAGAAAGACTGTACTTCAAAA ATAGGACAAACAAATGCTCGTGGCAAAGATTTGGATACAGACTTCACAAATAATGCCTCCCAACCTGAG ACCAAAGCCATCATTGAAAATTTGATTCAAAAACAGGACTTTAGTCTTTCTGTTGCCTTAGATGGTGGT TCCATGCTGGTCACATATCCTTATGACAAGCCAGTACAGACAGTGGAAAATAAAGAGACTCTGAAGCAT TTGGCATCTCTTTATGCAAATAATCATCCATCCATGCACATGGGTCAGCCCAGTTGCCCAAATAAATCA GATGAGAATATTCCAGGAGGAGTAATGCGTGGAGCAGAATGGCATAGTCACCTGGGCAGCATGAAGGAT TATAGTGTCACCTATGGCCATTGTCCGGAAATCACAGTATACACAAGCTGCTGTTACTTTCCTAGTGCT GCACGACTCCCTTCCTTGTGGGCAGACAATAAGAGATCTCTTCTTAGTATGTTAGTGGAGGTTCACAAG GGAGTTCATGGATTTGTTAAAGATAAGACTGGAAAGCCAATCTCTAAAGCAGTCATTGTACTTAATGAA GGAATAAAGGTACAAACAAAAGAGGGAGGTTATTTCCATGTACTCTTAGCGCCAGGTGTCCATAACATT ATTGCCATCGCTGATGGGTACCAGCAACAACATTCACAGGTCTTTGTGCATCATGATGCAGCTAGTTCT GTGGTGATAGTCTTTGACACAGATAACCGGATATTTGGTTTGCCAAGGGAGCTTGTGGTAACTGTATCA GGTGCTACTATGTCGGCATTGATCCTAACAGCTTGCATTATTTGGTGCATCTGCTCAATCAAGTCTAAT AGACACAAGGATGGCTTTCATCGGCTCAGGCAGCATCATGATGAGTATGAAGATGAAATTCGCATGATG TCTACCGGCTCCAAGAAGTCCCTCCTAAGCCATGAGTTCCAGGATGAAACAGACACTGAAGAGGAAACA TTATATTCTAGCAAACATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001199775
Insert Size	3402 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199775.1
RefSeq Size	8422 bp
RefSeq ORF	3402 bp
Locus ID	1362
UniProt ID	O75976
Protein Families	Druggable Genome, Protease, Transmembrane
MW	126.5 kDa
Gene Summary	The metallocarboxypeptidase family of enzymes is divided into 2 subfamilies based on sequence similarities. The pancreatic carboxypeptidase-like and the regulatory B-type carboxypeptidase subfamilies. Carboxypeptidase D has been identified as a regulatory B-type carboxypeptidase. CPD is a homolog of duck gp180, a hepatitis B virus-binding protein. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR and coding region and uses an alternate start codon compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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