HCE (RNGTT) (NM_001286428) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CAP1A; hCAP; HCE; HCE1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238659 representing NM_001286428 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTGTTGGTGGACCTGACAAATACTTCAAGGTTCTATGACCGAAATGACATAGAAAAAGAAGGAA TCAAATATATAAAACTTCAGTGTAAAGGACATGGTGAGTGCCCTACCACTGAGAATACTGAGACCTTTAT TCGTCTGTGTGAGCGGTTTAATGAAAGAAATCCACCTGAACTTATAGGTGTTCATTGTACTCATGGCTTC AATCGCACTGGTTTCCTCATATGTGCCTTTTTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCAGTTG CTACTTTTGCCCAAGCCAGACCACCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCGGTA TGGTGACATAGAGGAAGCACCACCCCCACCTCTATTGCCAGATTGGTGTTTTGAGGATGATGAAGACGAA GATGAGGATGAGGATGGAAAGAAGGAATCAGAACCCGGGTCAAGTGCTTCTTTTGGCAAAAGGAGAAAAG AACGGTTAAAACTGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGTGTAACTCAAGTAACAACACA ACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTCTGTGGCTGGGAAGGGTCTGGATTCCCTGGA GCACAGCCTGTTTCCATGGACAAGCAAAATATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGA AAGCAGATGGTACTCGGTACATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAA TTCAGTATTTCATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACT CTCTTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTGATATATGACA TAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGTCTGCAGTGTATAGAACGAGA AATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTCATTGACAAAACACAGGAACCATTTAGCGTC AGAAATAAGCCGTTTTTTGACATCTGTACTTCAAGAAAGAAATACAAACCTGGTCGATGTGATGATATTT TGAAATGGAAGCCTCCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGG GTTACTTCCTCAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAAATCAAGGTG ACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAGAACAACAGCTGGGTCTTCA TGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAACACTGCCATGGCTGTGTGTAACAGCATCTC AAACCCTGTCACCAAGGAGATGCTGTTTGAGTTCATCGACAGATGTACTGCAGCTTCTCAAGGACAGAAG CGAAAACATCATCTGGACCCTGACACGGAGCTCATGCCACCACCACCTCCCAAAAGACCACGCCCTTTAA CC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238659 representing NM_001286428 Red=Cloning site Green=Tags(s) MGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHGF NRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPLLPDWCFEDDEDE DEDEDGKKESEPGSSASFGKRRKERLKLGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPG AQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNT LLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSV RNKPFFDICTSRKKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKV TKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTAASQGQK RKHHLDPDTELMPPPPPKRPRPLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286428
ORF Size	1542 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286428.2
RefSeq Size	4662 bp
RefSeq ORF	1545 bp
Locus ID	8732
UniProt ID	O60942
Protein Families	Druggable Genome, Phosphatase
MW	59.5 kDa
Gene Summary	Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.[UniProtKB/Swiss-Prot Function]

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