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HCE (RNGTT) (NM_001286428) Human Tagged ORF Clone
CAT＃: RG238659
- TrueORF®
RNGTT (tGFP-tagged) - Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), transcript variant 3
ORF Plasmid: DDK
"NM_001286428" in other vectors (2)
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CNY 4,940.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAP1A; hCAP; HCE; HCE1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG238659 representing NM_001286428.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCTTGTTGGTGGACCTGACAAATACTTCAAGGTTCTATGACCGAAATGACATAGAAAAAGAAGGA ATCAAATATATAAAACTTCAGTGTAAAGGACATGGTGAGTGCCCTACCACTGAGAATACTGAGACCTTT ATTCGTCTGTGTGAGCGGTTTAATGAAAGAAATCCACCTGAACTTATAGGTGTTCATTGTACTCATGGC TTCAATCGCACTGGTTTCCTCATATGTGCCTTTTTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCA GTTGCTACTTTTGCCCAAGCCAGACCACCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGT CGGTATGGTGACATAGAGGAAGCACCACCCCCACCTCTATTGCCAGATTGGTGTTTTGAGGATGATGAA GACGAAGATGAGGATGAGGATGGAAAGAAGGAATCAGAACCCGGGTCAAGTGCTTCTTTTGGCAAAAGG AGAAAAGAACGGTTAAAACTGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGTGTAACTCAAGTA ACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTCTGTGGCTGGGAAGGGTCTGGA TTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAAAATATTAAACTTTTAGACCTGAAGCCATACAAA GTAAGCTGGAAAGCAGATGGTACTCGGTACATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATT GATAGAGACAATTCAGTATTTCATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCAT TTATCAAATACTCTCTTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATAT TTGATATATGACATAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGTCTGCAG TGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTCATTGACAAAACACAG GAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACTTCAAGAAAGAAATACAAACCTGGT CGATGTGATGATATTTTGAAATGGAAGCCTCCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACA AGAATGGGAGGAGAAGGGTTACTTCCTCAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCC TTTGCACAAATCAAGGTGACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAG AACAACAGCTGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAACACTGCCATG GCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAGTTCATCGACAGATGTACT GCAGCTTCTCAAGGACAGAAGCGAAAACATCATCTGGACCCTGACACGGAGCTCATGCCACCACCACCT CCCAAAAGACCACGCCCTTTAACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG238659
Blue=ORF Red=Cloning site Green=Tag(s) MGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHG FNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPLLPDWCFEDDE DEDEDEDGKKESEPGSSASFGKRRKERLKLGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSG FPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMH LSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQ EPFSVRNKPFFDICTSRKKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERP FAQIKVTKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCT AASQGQKRKHHLDPDTELMPPPPPKRPRPLT TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001286428 |
| ORF Size | 1542 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001286428.2 |
| RefSeq Size | 4662 bp |
| RefSeq ORF | 1545 bp |
| Locus ID | 8732 |
| UniProt ID | O60942 |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 59.5 kDa |
| Gene Summary | Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
