TLE4 (NM_001282749) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BCE-1; BCE1; E(spI); E(spl); ESG; ESG4; Grg-4; GRG4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239471 representing NM_001282749 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACCAGACACCCGGCACCGCATCAGCCTGCTCAACCCT TTAAATTTACAATTTCCGAATCCTGTGATCGGATTAAGGAAGAGTTTCAGTTTTTACAGGCTCAATACCA CAGTCTGAAGCTGGAATGTGAGAAACTCGCCAGTGAGAAGACAGAGATGCAGCGGCATTATGTCATGTAT TATGAAATGTCCTATGGGTTGAATATAGAAATGCACAAGCAGGCAGAGATTGTCAAGAGGCTGAATGCTA TCTGTGCACAAGTCATTCCTTTCCTGTCCCAAGAGCAACAACTCCAGGCCCAGCATTTATCACATGGACA TGGTCTCCCCGTACCTCTGACTCCACACCCTTCAGGGCTCCAGCCCCCTGCCATTCCACCCATCGGTAGC AGTGCCGGGCTTCTGGCCCTCTCCAGTGCTCTAGGAGGTCAGTCCCATCTTCCAATTAAAGATGAGAAGA AGCACCATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCAGTATCCCCATCAGCCAG TTTCCGAGGTGCTGAGAAGCACAGAAACTCCGCAGACTACTCCTCAGAGAGCAAAAAGCAGAAAACTGAA GAAAAGGAAATTGCAGCTCGTTATGACAGCGATGGTGAGAAAAGTGATGACAACTTGGTGGTTGACGTTT CCAATGAGGATCCATCTTCCCCTCGAGGGAGCCCAGCACATTCCCCCAGAGAGAATGGCCTAGACAAGAC ACGCCTGCTCAAGAAAGATGCCCCGATTAGTCCAGCCTCTATTGCATCTTCCAGCAGTACTCCCTCCTCC AAATCCAAAGAACTTAGCCTTAATGAAAAATCTACTACTCCCGTCTCAAAGTCCAATACCCCTACTCCAC GAACTGATGCGCCCACCCCAGGCAGTAACTCTACTCCCGGATTGAGGCCTGTACCTGGAAAACCACCAGG AGTTGACCCTTTGGCCTCAAGCCTAAGGACCCCAATGGCAGTACCTTGTCCATATCCAACTCCATTTGGG ATTGTGCCCCATGCTGGAATGAACGGAGAGCTGACCAGCCCCGGAGCGGCCTACGCTGGGCTCCACAACA TCTCCCCTCAGATGAGCGCAGCTGCTGCCGCCGCCGCTGCTGCTGCTGCCTATGGGAGATCACCAGTGGT GGGATTTGATCCACACCATCACATGCGTGTGCCAGCAATACCTCCAAACCTGACAGGCATTCCAGGAGGA AAACCAGCATACTCCTTCCATGTTAGCGCAGATGGTCAGATGCAGCCTGTCCCTTTTCCACCCGACGCCC TCATCGGACCTGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGC GGTGACCATCAGCAACCCCACGAGACACGTGTACACGGGTGGGAAGGGCTGCGTCAAGGTCTGGGACATC AGCCACCCAGGCAATAAGAGTCCTGTCTCCCAGCTCGACTGTCTGAACAGGGATAACTACATCCGTTCCT GCAGATTGCTCCCTGATGGTCGCACCCTAATTGTTGGAGGGGAAGCCAGTACTTTGTCCATTTGGGACCT GGCGGCTCCAACCCCACGCATCAAGGCAGAGCTGACATCCTCGGCCCCCGCCTGCTATGCCCTGGCCATC AGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGGGATCTGCACAACC AGACCTTGGTGAGGCAATTCCAGGGCCACACAGATGGAGCCAGCTGTATTGACATTTCTAATGATGGCAC CAAGCTCTGGACAGGTGGTTTGGACAACACGGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAG CAGCACGACTTCACCTCCCAGATCTTTTCTCTGGGCTACTGCCCAACTGGAGAGTGGCTTGCAGTGGGGA TGGAGAACAGCAATGTGGAAGTTTTGCATGTCACCAAGCCAGACAAATACCAACTACATCTTCATGAGAG CTGTGTGCTGTCGCTCAAGTTTGCCCATTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACAACCTTCTG AATGCCTGGAGAACACCTTATGGGGCCAGTATATTCCAGTCCAAAGAATCCTCATCGGTGCTTAGCTGTG ACATCTCCGTGGACGACAAATACATTGTCACTGGCTCTGGGGATAAGAAGGCCACAGTTTATGAAGTTAT TTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239471 representing NM_001282749 Red=Cloning site Green=Tags(s) MIRDLSKMYPQTRHPAPHQPAQPFKFTISESCDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMY YEMSYGLNIEMHKQAEIVKRLNAICAQVIPFLSQEQQLQAQHLSHGHGLPVPLTPHPSGLQPPAIPPIGS SAGLLALSSALGGQSHLPIKDEKKHHDNDHQRDRDSIKSSSVSPSASFRGAEKHRNSADYSSESKKQKTE EKEIAARYDSDGEKSDDNLVVDVSNEDPSSPRGSPAHSPRENGLDKTRLLKKDAPISPASIASSSSTPSS KSKELSLNEKSTTPVSKSNTPTPRTDAPTPGSNSTPGLRPVPGKPPGVDPLASSLRTPMAVPCPYPTPFG IVPHAGMNGELTSPGAAYAGLHNISPQMSAAAAAAAAAAAYGRSPVVGFDPHHHMRVPAIPPNLTGIPGG KPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDI SHPGNKSPVSQLDCLNRDNYIRSCRLLPDGRTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAI SPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQ QHDFTSQIFSLGYCPTGEWLAVGMENSNVEVLHVTKPDKYQLHLHESCVLSLKFAHCGKWFVSTGKDNLL NAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282749
ORF Size	2244 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282749.1, NP_001269678.1
RefSeq Size	5003 bp
RefSeq ORF	2247 bp
Locus ID	7091
UniProt ID	Q04727
Protein Families	Transcription Factors
MW	81.5 kDa
Gene Summary	Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.