TLE4 (NM_001282749) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCE-1; BCE1; E(spI); E(spl); ESG; ESG4; Grg-4; GRG4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282749, the custom clone sequence may differ by one or more nucleotides ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACCAGACACCCGGCACCGCATCAGCCTGCTCAACCCT TTAAATTTACAATTTCCGAATCCTGTGATCGGATTAAGGAAGAGTTTCAGTTTTTACAGGCTCAATACCA CAGTCTGAAGCTGGAATGTGAGAAACTCGCCAGTGAGAAGACAGAGATGCAGCGGCATTATGTCATGTAT TATGAAATGTCCTATGGGTTGAATATAGAAATGCACAAGCAGGCAGAGATTGTCAAGAGGCTGAATGCTA TCTGTGCACAAGTCATTCCTTTCCTGTCCCAAGAGCAACAACTCCAGGCCCAGCATTTATCACATGGACA TGGTCTCCCCGTACCTCTGACTCCACACCCTTCAGGGCTCCAGCCCCCTGCCATTCCACCCATCGGTAGC AGTGCCGGGCTTCTGGCCCTCTCCAGTGCTCTAGGAGGTCAGTCCCATCTTCCAATTAAAGATGAGAAGA AGCACCATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCAGTATCCCCATCAGCCAG TTTCCGAGGTGCTGAGAAGCACAGAAACTCCGCAGACTACTCCTCAGAGAGCAAAAAGCAGAAAACTGAA GAAAAGGAAATTGCAGCTCGTTATGACAGCGATGGTGAGAAAAGTGATGACAACTTGGTGGTTGACGTTT CCAATGAGGATCCATCTTCCCCTCGAGGGAGCCCAGCACATTCCCCCAGAGAGAATGGCCTAGACAAGAC ACGCCTGCTCAAGAAAGATGCCCCGATTAGTCCAGCCTCTATTGCATCTTCCAGCAGTACTCCCTCCTCC AAATCCAAAGAACTTAGCCTTAATGAAAAATCTACTACTCCCGTCTCAAAGTCCAATACCCCTACTCCAC GAACTGATGCGCCCACCCCAGGCAGTAACTCTACTCCCGGATTGAGGCCTGTACCTGGAAAACCACCAGG AGTTGACCCTTTGGCCTCAAGCCTAAGGACCCCAATGGCAGTACCTTGTCCATATCCAACTCCATTTGGG ATTGTGCCCCATGCTGGAATGAACGGAGAGCTGACCAGCCCCGGAGCGGCCTACGCTGGGCTCCACAACA TCTCCCCTCAGATGAGCGCAGCTGCTGCCGCCGCCGCTGCTGCTGCTGCCTATGGGAGATCACCAGTGGT GGGATTTGATCCACACCATCACATGCGTGTGCCAGCAATACCTCCAAACCTGACAGGCATTCCAGGAGGA AAACCAGCATACTCCTTCCATGTTAGCGCAGATGGTCAGATGCAGCCTGTCCCTTTTCCACCCGACGCCC TCATCGGACCTGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGC GGTGACCATCAGCAACCCCACGAGACACGTGTACACGGGTGGGAAGGGCTGCGTCAAGGTCTGGGACATC AGCCACCCAGGCAATAAGAGTCCTGTCTCCCAGCTCGACTGTCTGAACAGGGATAACTACATCCGTTCCT GCAGATTGCTCCCTGATGGTCGCACCCTAATTGTTGGAGGGGAAGCCAGTACTTTGTCCATTTGGGACCT GGCGGCTCCAACCCCACGCATCAAGGCAGAGCTGACATCCTCGGCCCCCGCCTGCTATGCCCTGGCCATC AGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGGGATCTGCACAACC AGACCTTGGTGAGGCAATTCCAGGGCCACACAGATGGAGCCAGCTGTATTGACATTTCTAATGATGGCAC CAAGCTCTGGACAGGTGGTTTGGACAACACGGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAG CAGCACGACTTCACCTCCCAGATCTTTTCTCTGGGCTACTGCCCAACTGGAGAGTGGCTTGCAGTGGGGA TGGAGAACAGCAATGTGGAAGTTTTGCATGTCACCAAGCCAGACAAATACCAACTACATCTTCATGAGAG CTGTGTGCTGTCGCTCAAGTTTGCCCATTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACAACCTTCTG AATGCCTGGAGAACACCTTATGGGGCCAGTATATTCCAGTCCAAAGAATCCTCATCGGTGCTTAGCTGTG ACATCTCCGTGGACGACAAATACATTGTCACTGGCTCTGGGGATAAGAAGGCCACAGTTTATGAAGTTAT TTATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282749
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282749.1, NP_001269678.1
RefSeq Size	5003 bp
RefSeq ORF	2247 bp
Locus ID	7091
UniProt ID	Q04727
Protein Families	Transcription Factors
Gene Summary	Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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