DMTF1 (NM_021145) Human Tagged ORF Clone
CAT#: RG209791
- TrueORF®
DMTF1 (tGFP-tagged) - Human cyclin D binding myb-like transcription factor 1 (DMTF1), transcript variant 1
ORF Plasmid: DDK
"NM_021145" in other vectors (6)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DMP1; DMTF; hDMP1; MRUL |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209791 representing NM_021145
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACAGTGGAAGAGGATTCTGACACAGTAACAGTAGAAACTGTGAACTCTGTGACTTTGACTCAGG ACACAGAAGGGAATCTCATTCTTCACTGCCCTCAGAATGAAGCGGATGAAATAGACTCAGAAGATAGTAT TGAACCTCCACATAAAAGGCTTTGTTTGTCCTCTGAGGATGATCAGAGTATTGATGATTCTACTCCTTGC ATATCAGTTGTTGCACTTCCACTTTCAGAAAATGATCAGAGCTTTGAAGTGACCATGACTGCAACCACAG AAGTAGCAGATGATGAGGTTACTGAGGGGACTGTGACACAGATACAGATTTTGCAGAATGAGCAACTAGA TGAAATATCTCCCTTGGGTAACGAGGAAGTTTCAGCAGTTAGCCAAGCATGGTTTACAACTAAAGAAGAT AAGGATTCTCTGACTAATAAAGGACATAAATGGAAGCAGGGGATGTGGTCCAAGGAAGAAATTGATATTT TGATGAACAATATTGAACGCTATCTTAAGGCACGCGGAATAAAAGATGCTACAGAAATCATCTTTGAGAT GTCAAAAGACGAAAGAAAAGATTTCTACAGGACTATAGCATGGGGTCTGAACCGGCCTTTGTTTGCAGTT TATAGAAGAGTGCTTCGCATGTATGATGACAGAAACCATGTGGGAAAATATACACCTGAAGAAATTGAGA AGCTCAAGGAGCTCCGGATAAAGCATGGCAATGACTGGGCAACAATAGGGGCGGCGCTAGGAAGAAGTGC ATCTTCTGTCAAAGATCGGTGCCGACTGATGAAGGATACTTGCAACACAGGGAAGTGGACAGAAGAAGAA GAAAAGAGACTTGCAGAAGTGGTTCATGAGTTGACAAGCACTGAGCCAGGTGACATAGTCACACAGGGTG TGTCTTGGGCAGCTGTGGCTGAACGAGTCGGTACCCGCTCAGAAAAGCAATGTCGTTCTAAATGGCTCAA CTACCTGAATTGGAAACAGAGTGGGGGTACTGAATGGACCAAGGAAGATGAAATCAATCTCATCCTCAGG ATAGCAGAACTTGATGTAGCTGATGAAAATGACATTAACTGGGATCTGTTAGCTGAGGGATGGAGTAGTG TCCGTTCACCACAATGGCTACGAAGTAAATGGTGGACCATCAAAAGGCAAATTGCAAACCATAAGGATGT TTCGTTCCCTGTCTTAATAAAAGGTCTTAAACAGTTACATGAGAACCAAAAAAACAACCCAACGCTTTTG GAGAATAAATCAGGATCTGGAGTTCCAAACAGTAATACCAATTCCAGTGTGCAGCATGTTCAGATAAGAG TTGCCCGCTTGGAAGATAATACAGCCATCTCTTCTAGCCCCATGGCAGCATTGCAGATTCCAGTCCAGAT CACCCATGTTTCTTCAGCAGACTCTCCTGCTACCGTTGACTCAGAAACAATAACACTAAACAGTGGAACA CTACAGACATTTGAGATTCTTCCCTCTTTCCATCTACAGCCCACTGGCACTCCAGGCACCTACCTACTTC AAACAAGCTCAAGCCAAGGCCTTCCCCTAACTCTGACTGCTAGTCCCACAGTAACCCTGACAGCTGCTGC TCCTGCTTCTCCTGAACAGATTATTGTTCATGCTTTATCCCCAGAACATTTGTTGAACACAAGTGATAAT GTTACAGTGCAGTGTCACACACCAAGAGTCATCATTCAGACTGTTGCCACAGAGGACATCACTTCTTCCA TATCCCAAGCAGAACTGACAGTCGATAGTGATATTCAGTCATCTGATTTTCCTGAGCCTCCAGACGCCCT AGAAGCAGACACTTTCCCAGATGAAGTTCATCACCCTAAGATGACTGTGGAGCCATCATTTAATGATGCT CATGTATCCAAATTCAGTGACCAAAATAGCACAGAACTGATGAATAGTGTTATGGTCAGAACAGAAGAAG AAATCTCTGACACCGACCTTAAACAAGAGGAATCACCCTCTGATTTAGCCAGTGCTTATGTTACTGAGGG TTTAGAGTCTCCCACTATAGAAGAACAAGTTGATCAAACAATTGATGATGAAACAATACTTATCGTTCCT TCACCACATGGCTTTATCCAGGCATCTGATGTTATAGATACTGAATCTGTCTTGCCTTTGACAACACTAA CAGATCCCATACTCCAACATCATCAGGAAGAATCAAATATCATTGGATCATCCTTGGGCAGTCCTGTTTC AGAAGATTCAAAGGATGTCGAAGATTTGGTAAACTGTCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209791 representing NM_021145
Red=Cloning site Green=Tags(s) MSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPC ISVVALPLSENDQSFEVTMTATTEVADDEVTEGTVTQIQILQNEQLDEISPLGNEEVSAVSQAWFTTKED KDSLTNKGHKWKQGMWSKEEIDILMNNIERYLKARGIKDATEIIFEMSKDERKDFYRTIAWGLNRPLFAV YRRVLRMYDDRNHVGKYTPEEIEKLKELRIKHGNDWATIGAALGRSASSVKDRCRLMKDTCNTGKWTEEE EKRLAEVVHELTSTEPGDIVTQGVSWAAVAERVGTRSEKQCRSKWLNYLNWKQSGGTEWTKEDEINLILR IAELDVADENDINWDLLAEGWSSVRSPQWLRSKWWTIKRQIANHKDVSFPVLIKGLKQLHENQKNNPTLL ENKSGSGVPNSNTNSSVQHVQIRVARLEDNTAISSSPMAALQIPVQITHVSSADSPATVDSETITLNSGT LQTFEILPSFHLQPTGTPGTYLLQTSSSQGLPLTLTASPTVTLTAAAPASPEQIIVHALSPEHLLNTSDN VTVQCHTPRVIIQTVATEDITSSISQAELTVDSDIQSSDFPEPPDALEADTFPDEVHHPKMTVEPSFNDA HVSKFSDQNSTELMNSVMVRTEEEISDTDLKQEESPSDLASAYVTEGLESPTIEEQVDQTIDDETILIVP SPHGFIQASDVIDTESVLPLTTLTDPILQHHQEESNIIGSSLGSPVSEDSKDVEDLVNCH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021145 |
ORF Size | 2280 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021145.2, NP_066968.2 |
RefSeq Size | 3998 bp |
RefSeq ORF | 2283 bp |
Locus ID | 9988 |
UniProt ID | Q9Y222 |
Domains | myb_DNA-binding |
Protein Families | Transcription Factors |
Gene Summary | This gene encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209791 | DMTF1 (Myc-DDK-tagged)-Human cyclin D binding myb-like transcription factor 1 (DMTF1), transcript variant 1 |
CNY 5,568.00 |
|
RC209791L1 | Lenti-ORF clone of DMTF1 (Myc-DDK-tagged)-Human cyclin D binding myb-like transcription factor 1 (DMTF1), transcript variant 1 |
CNY 7,968.00 |
|
RC209791L2 | Lenti-ORF clone of DMTF1 (mGFP-tagged)-Human cyclin D binding myb-like transcription factor 1 (DMTF1), transcript variant 1 |
CNY 5,890.00 |
|
RC209791L3 | Lenti-ORF clone of DMTF1 (Myc-DDK-tagged)-Human cyclin D binding myb-like transcription factor 1 (DMTF1), transcript variant 1 |
CNY 5,890.00 |
|
RC209791L4 | Lenti-ORF clone of DMTF1 (mGFP-tagged)-Human cyclin D binding myb-like transcription factor 1 (DMTF1), transcript variant 1 |
CNY 5,890.00 |
|
SC107975 | DMTF1 (untagged)-Human cyclin D binding myb-like transcription factor 1 (DMTF1), transcript variant 1 |
CNY 5,576.00 |