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NTH1 (NTHL1) (NM_002528) Human Tagged ORF Clone
CAT＃: RG214598
- TrueORF®
NTHL1 (tGFP-tagged) - Human nth endonuclease III-like 1 (E. coli) (NTHL1)
ORF Plasmid: DDK
"NM_002528" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
| Cited in 2 publications. |
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FAP3; hNTH1; NTH1; OCTS3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214598 representing NM_002528
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTAGTCCGCAGGAGTCCGGCATGACCGCCTTGAGCGCGAGGATGCTGACCCGGAGCCGGAGCCTGG GACCCGGGGCTGGGCCGCGGGGGTGTAGGGAGGAGCCCGGGCCTCTCCGGAGAAGAGAGGCTGCAGCAGA AGCGAGGAAAAGCCACAGCCCCGTGAAGCGTCCGCGGAAAGCACAGAGACTGCGTGTGGCCTATGAGGGC TCGGACAGTGAGAAAGGTGAGGGGGCTGAGCCCCTCAAGGTGCCAGTCTGGGAGCCCCAGGACTGGCAGC AACAGCTGGTCAACATCCGTGCCATGAGGAACAAAAAGGATGCACCTGTGGACCATCTGGGGACTGAGCA CTGCTATGACTCCAGTGCCCCCCCAAAGGTACGCAGGTACCAGGTGCTGCTGTCACTGATGCTCTCCAGC CAAACCAAAGACCAGGTGACGGCGGGCGCCATGCAGCGACTGCGGGCGCGGGGCCTGACGGTGGACAGCA TCCTGCAGACAGATGATGCCACGCTGGGCAAGCTCATCTACCCCGTCGGTTTCTGGAGGAGCAAGGTGAA ATACATCAAGCAGACCAGCGCCATCCTGCAGCAGCACTACGGTGGGGACATCCCAGCCTCTGTGGCCGAG CTGGTGGCGCTGCCGGGTGTTGGGCCCAAGATGGCACACCTGGCTATGGCTGTGGCCTGGGGCACTGTGT CAGGCATTGCAGTGGACACGCATGTGCACAGAATCGCCAACAGGCTGAGGTGGACCAAGAAGGCAACCAA GTCCCCAGAGGAGACCCGCGCCGCCCTGGAGGAGTGGCTGCCTAGGGAGCTGTGGCACGAGATCAATGGA CTCTTGGTGGGCTTCGGCCAGCAGACCTGTCTGCCTGTGCACCCTCGCTGCCACGCCTGCCTCAACCAAG CCCTCTGCCCGGCCGCCCAGGGTCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214598 representing NM_002528
Red=Cloning site Green=Tags(s) MCSPQESGMTALSARMLTRSRSLGPGAGPRGCREEPGPLRRREAAAEARKSHSPVKRPRKAQRLRVAYEG SDSEKGEGAEPLKVPVWEPQDWQQQLVNIRAMRNKKDAPVDHLGTEHCYDSSAPPKVRRYQVLLSLMLSS QTKDQVTAGAMQRLRARGLTVDSILQTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAE LVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEING LLVGFGQQTCLPVHPRCHACLNQALCPAAQGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002528 |
| ORF Size | 936 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002528.6 |
| RefSeq Size | 1097 bp |
| RefSeq ORF | 915 bp |
| Locus ID | 4913 |
| UniProt ID | P78549 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Base excision repair |
| Gene Summary | The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Overexpression of the base excision repair NTHL1 glycosylase causes genomic instability and early cellular hallmarks of cancer
,Limpose, KL;Trego, KS;Li, Z;Leung, SW;Sarker, AH;Shah, JA;Ramalingam, SS;Werner, EM;Dynan, WS;Cooper, PK;Corbett, AH;Doetsch, PW;,
Nucleic Acids Res.
,PubMed ID 29522130
[NTHL1]
|
|
Identification of SUMO modification sites in the base excision repair protein, Ntg1
,Swartzlander, DB;McPherson, AJ;Powers, HR;Limpose, KL;Kuiper, EG;Degtyareva, NP;Corbett, AH;Doetsch, PW;,
DNA Repair (Amst.) 2016
,PubMed ID 27839712
[NTHL1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214598 | NTHL1 (Myc-DDK-tagged)-Human nth endonuclease III-like 1 (E. coli) (NTHL1) |
CNY 2,400.00 |
|
| RC214598L3 | Lenti ORF clone of Human nth endonuclease III-like 1 (E. coli) (NTHL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214598L4 | Lenti ORF clone of Human nth endonuclease III-like 1 (E. coli) (NTHL1), mGFP tagged |
CNY 5,890.00 |
|
| SC303213 | NTHL1 (untagged)-Human nth endonuclease III-like 1 (E. coli) (NTHL1) |
CNY 2,400.00 |
