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EGFLAM (NM_152403) Human Tagged ORF Clone
CAT＃: RG220450
- TrueORF®
EGFLAM (tGFP-tagged) - Human EGF-like, fibronectin type III and laminin G domains (EGFLAM), transcript variant 1
ORF Plasmid: DDK
"NM_152403" in other vectors (6)
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CNY 8,624.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AGRINL; AGRNL; PIKA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220450 representing NM_152403
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTAATCCGAGGCGTCTTGCTCCGGCTCCTGCTCCTGGCTTCCAGCCTCGGACCCGGCGCGGTGT CGCTCCGAGCGGCCATCCGAAAACCAGGCAAGGTAGGGCCTCCTCTTGACATCAAGCTGGGCGCATTGAA CTGTACGGCTTTCAGCATCCAGTGGAAAATGCCAAGGCATCCTGGAAGTCCCATCCTTGGGTACACTGTC TTTTACTCTGAGGTTGGCGCAGATAAATCCCTGCAGGAGCAGTTGCACAGCGTGCCTCTCAGCCGGGACA TCCCGACCACGGAGGAAGTGATTGGAGATTTGAAACCAGGCACTGAATATCGTGTGAGCATAGCAGCTTA CAGCCAGGCTGGCAAAGGGCGGCTGAGCTCTCCTCGGCATGTCACCACTTTGTCCCAAGATTCCTGCCTG CCTCCTGCAGCTCCCCAGCAGCCACATGTCATTGTGGTTTCGGATTCTGAGGTGGCCCTGTCTTGGAAAC CTGGAGCGAGTGAAGGAAGCGCCCCTATTCAGTACTATTCTGTGGAATTCATCAGGCCAGATTTCGACAA GAAGTGGACCTCAATCCATGAGCGGATCCAGATGGACTCCATGGTTATCAAGGGCCTCGATCCAGATACC AACTACCAGTTTGCCGTGAGGGCAATGAATTCCCATGGCCCCAGCCCCCGCAGCTGGCCCAGTGACATCA TCCGGACCCTCTGCCCTGAGGAGGCGGGAAGTGGCCGCTATGGACCCCGTTATATCACCGACATGGGAGC TGGTGAGGATGATGAAGGATTTGAAGACGACTTAGATTTGGATATTTCCTTTGAGGAGGTTAAACCACTT CCTGCTACCAAAGGAGGGAATAAGAAATTTTTGGTGGAAAGCAAGAAGATGTCTATATCTAACCCAAAGA CCATTTCTAGGCTCATCCCCCCTACCTCAGCATCTCTCCCTGTGACCACGGTGGCTCCCCAGCCCATTCC CATACAGAGAAAGGGGAAGAATGGTGTGGCCATAATGTCAAGGCTCTTTGACATGCCTTGTGATGAAACT CTCTGCTCTGCTGACAGCTTCTGTGTCAATGACTACACCTGGGGGGGCTCGCGATGCCAGTGCACCCTGG GCAAAGGTGGTGAGAGCTGCTCAGAAGATATTGTTATCCAGTATCCTCAGTTCTTTGGCCACTCCTATGT AACGTTTGAACCTCTGAAGAATTCTTATCAGGCATTTCAAATTACTCTTGAATTTAGGGCGGAGGCAGAG GATGGCTTACTGCTCTACTGTGGGGAGAACGAACACGGGAGGGGGGATTTCATGTCCCTGGCTATCATCC GACGCTCCCTGCAGTTCAGGTTTAATTGTGGAACTGGGGTTGCCATCATCGTAAGTGAGACCAAAATCAA ACTAGGGGGTTGGCACACGGTTATGCTCTACAGAGATGGGCTGAACGGGCTGCTGCAGCTGAACAATGGC ACCCCAGTGACAGGCCAGTCTCAGGGCCAATACAGTAAAATTACTTTCCGGACACCTCTCTATCTTGGTG GCGCTCCCAGCGCTTACTGGTTGGTTAGAGCAACAGGGACAAACCGAGGCTTTCAAGGCTGTGTGCAGTC GCTCGCTGTGAATGGGAGGAGAATTGACATGAGGCCCTGGCCCCTGGGAAAAGCACTCAGTGGGGCTGAT GTGGGGGAATGCAGCAGTGGAATCTGTGATGAGGCCTCGTGCATCCATGGTGGCACCTGCACAGCAATCA AAGCCGACTCCTACATTTGCCTCTGTCCCCTTGGGTTTAAAGGTCGACACTGTGAAGATGCTTTCACCTT GACCATTCCTCAGTTCAGAGAGTCTCTGAGATCTTACGCTGCAACTCCCTGGCCACTGGAGCCCCAGCAT TACCTTTCCTTCATGGAATTTGAGATCACATTTCGGCCAGACTCAGGAGATGGTGTCCTCCTGTACAGCT ATGACACAGGCAGCAAAGACTTCCTGTCCATCAACTTGGCAGGGGGCCACGTGGAGTTCCGCTTTGACTG TGGCTCTGGGACCGGTGTCCTCAGGAGTGAAGATCCCCTCACCCTGGGCAACTGGCACGAGCTTCGTGTA TCTCGCACAGCAAAGAATGGAATCTTACAGGTGGATAAGCAGAAGATAGTGGAGGGAATGGCAGAGGGAG GCTTCACACAGATTAAGTGCAACACAGACATTTTCATTGGCGGAGTCCCCAATTATGATGATGTGAAGAA GAACTCGGGTGTCCTGAAGCCTTTCAGCGGGAGCATCCAGAAGATCATCCTGAATGACCGAACCATCCAT GTGAAGCATGACTTCACCTCCGGAGTGAATGTGGAGAATGCGGCCCACCCCTGTGTGAGAGCCCCTTGTG CCCATGGGGGCAGCTGCCGGCCCAGGAAGGAGGGCTATGACTGTGACTGCCCCTTGGGCTTTGAGGGGCT TCACTGCCAGAAAGCGATCATAGAAGCCATTGAGATCCCGCAGTTTATCGGCCGCAGTTACCTGACGTAT GACAACCCAGATATCTTGAAGAGGGTGTCAGGATCAAGATCAAATGTGTTCATGAGGTTTAAAACAACTG CCAAGGATGGCCTTTTGCTGTGGAGGGGAGACAGCCCCATGAGACCCAACAGCGACTTCATTTCCTTGGG CCTTCGGGATGGAGCCCTCGTGTTCAGCTATAACCTGGGCAGTGGTGTGGCATCCATCATGGTGAATGGC TCCTTCAACGATGGTCGGTGGCACCGAGTTAAGGCCGTTAGGGATGGCCAGTCAGGAAAGATAACCGTGG ATGACTATGGAGCCAGAACAGGCAAATCCCCAGGCATGATGCGGCAGCTTAACATCAATGGAGCTCTGTA TGTGGGTGGAATGAAGGAAATTGCTCTGCACACTAACAGGCAATATATGAGAGGGCTCGTGGGCTGTATC TCTCACTTCACCCTGTCCACCGATTACCACATTTCCCTCGTGGAAGATGCCGTGGATGGGAAAAACATCA ACACTTGTGGAGCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220450 representing NM_152403
Red=Cloning site Green=Tags(s) MDLIRGVLLRLLLLASSLGPGAVSLRAAIRKPGKVGPPLDIKLGALNCTAFSIQWKMPRHPGSPILGYTV FYSEVGADKSLQEQLHSVPLSRDIPTTEEVIGDLKPGTEYRVSIAAYSQAGKGRLSSPRHVTTLSQDSCL PPAAPQQPHVIVVSDSEVALSWKPGASEGSAPIQYYSVEFIRPDFDKKWTSIHERIQMDSMVIKGLDPDT NYQFAVRAMNSHGPSPRSWPSDIIRTLCPEEAGSGRYGPRYITDMGAGEDDEGFEDDLDLDISFEEVKPL PATKGGNKKFLVESKKMSISNPKTISRLIPPTSASLPVTTVAPQPIPIQRKGKNGVAIMSRLFDMPCDET LCSADSFCVNDYTWGGSRCQCTLGKGGESCSEDIVIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAE DGLLLYCGENEHGRGDFMSLAIIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVMLYRDGLNGLLQLNNG TPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGRRIDMRPWPLGKALSGAD VGECSSGICDEASCIHGGTCTAIKADSYICLCPLGFKGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQH YLSFMEFEITFRPDSGDGVLLYSYDTGSKDFLSINLAGGHVEFRFDCGSGTGVLRSEDPLTLGNWHELRV SRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGVLKPFSGSIQKIILNDRTIH VKHDFTSGVNVENAAHPCVRAPCAHGGSCRPRKEGYDCDCPLGFEGLHCQKAIIEAIEIPQFIGRSYLTY DNPDILKRVSGSRSNVFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNG SFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCI SHFTLSTDYHISLVEDAVDGKNINTCGAK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152403 |
| ORF Size | 3027 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152403.4 |
| RefSeq Size | 3952 bp |
| RefSeq ORF | 3030 bp |
| Locus ID | 133584 |
| UniProt ID | Q63HQ2 |
| Protein Families | Transmembrane |
| Gene Summary | Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220450 | EGFLAM (Myc-DDK-tagged)-Human EGF-like, fibronectin type III and laminin G domains (EGFLAM), transcript variant 1 |
CNY 7,024.00 |
|
| RC220450L1 | Lenti ORF clone of Human EGF-like, fibronectin type III and laminin G domains (EGFLAM), transcript variant 1, Myc-DDK-tagged |
CNY 9,424.00 |
|
| RC220450L2 | Lenti ORF clone of Human EGF-like, fibronectin type III and laminin G domains (EGFLAM), transcript variant 1, mGFP tagged |
CNY 11,970.00 |
|
| RC220450L3 | Lenti ORF clone of Human EGF-like, fibronectin type III and laminin G domains (EGFLAM), transcript variant 1, Myc-DDK-tagged |
CNY 11,970.00 |
|
| RC220450L4 | Lenti ORF clone of Human EGF-like, fibronectin type III and laminin G domains (EGFLAM), transcript variant 1, mGFP tagged |
CNY 11,970.00 |
|
| SC306386 | EGFLAM (untagged)-Human EGF-like, fibronectin type III and laminin G domains (EGFLAM), transcript variant 1 |
CNY 10,450.00 |
