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TREM2 (NM_018965) Human Tagged ORF Clone
CAT＃: RG221132
- TrueORF®
TREM2 (tGFP-tagged) - Human triggering receptor expressed on myeloid cells 2 (TREM2)
ORF Plasmid: DDK
"NM_018965" in other vectors (6)
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CNY 5,200.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PLOSL2; TREM-2; Trem2a; Trem2b; Trem2c |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221132 representing NM_018965
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCTCTCCGGCTGCTCATCTTACTCTTTGTCACAGAGCTGTCCGGAGCCCACAACACCACAGTGT TCCAGGGCGTGGCGGGCCAGTCCCTGCAGGTGTCTTGCCCCTATGACTCCATGAAGCACTGGGGGAGGCG CAAGGCCTGGTGCCGCCAGCTGGGAGAGAAGGGCCCATGCCAGCGTGTGGTCAGCACGCACAACTTGTGG CTGCTGTCCTTCCTGAGGAGGTGGAATGGGAGCACAGCCATCAAAGACGATACCCTGGGTGGCACTCTCA CCATTACGCTGCGGAATCTACAACCCCATGATGCGGGTCTCTACCAGTGCCAGAGCCTCCATGGCAGTGA GGCTGACACCCTCAGGAAGGTCCTGGTGGAGGTGCTGGCAGACCCCCTGGATCACCGGGATGCTGGAGAT CTCTGGTTCCCCGGGGAGTCTGAGAGCTTCGAGGATGCCCATGTGGAGCACAGCATCTCCAGGAGCCTCT TGGAAGGAGAAATCCCCTTCCCACCCACTTCCATCCTTCTCCTCCTGGCCTGCATCTTTCTCATCAAGAT TCTAGCAGCCAGCGCCCTCTGGGCTGCAGCCTGGCATGGACAGAAGCCAGGGACACATCCACCCAGTGAA CTGGACTGTGGCCATGACCCAGGGTATCAGCTCCAAACTCTGCCAGGGCTGAGAGACACG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221132 representing NM_018965
Red=Cloning site Green=Tags(s) MEPLRLLILLFVTELSGAHNTTVFQGVAGQSLQVSCPYDSMKHWGRRKAWCRQLGEKGPCQRVVSTHNLW LLSFLRRWNGSTAIKDDTLGGTLTITLRNLQPHDAGLYQCQSLHGSEADTLRKVLVEVLADPLDHRDAGD LWFPGESESFEDAHVEHSISRSLLEGEIPFPPTSILLLLACIFLIKILAASALWAAAWHGQKPGTHPPSE LDCGHDPGYQLQTLPGLRDT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018965 |
| ORF Size | 690 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018965.1, NP_061838.1 |
| RefSeq Size | 1041 bp |
| RefSeq ORF | 693 bp |
| Locus ID | 54209 |
| UniProt ID | Q9NZC2 |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Gene Summary | This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221132 | TREM2 (Myc-DDK-tagged)-Human triggering receptor expressed on myeloid cells 2 (TREM2) |
CNY 3,600.00 |
|
| RC221132L1 | Lenti ORF clone of Human triggering receptor expressed on myeloid cells 2 (TREM2), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC221132L2 | Lenti ORF clone of Human triggering receptor expressed on myeloid cells 2 (TREM2), mGFP tagged |
CNY 6,000.00 |
|
| RC221132L3 | Lenti ORF clone of Human triggering receptor expressed on myeloid cells 2 (TREM2), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC221132L4 | Lenti ORF clone of Human triggering receptor expressed on myeloid cells 2 (TREM2), mGFP tagged |
CNY 6,000.00 |
|
| SC113273 | TREM2 (untagged)-Human triggering receptor expressed on myeloid cells 2 (TREM2) |
CNY 3,600.00 |
