DMP1 (NM_001079911) Human Tagged ORF Clone
CAT#: RG222208
- TrueORF®
DMP1 (tGFP-tagged) - Human dentin matrix acidic phosphoprotein 1 (DMP1), transcript variant 2
ORF Plasmid: DDK
"NM_001079911" in other vectors (4)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ARHP; ARHR; DMP-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222208 representing NM_001079911
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATCAGCATCCTGCTCATGTTCCTTTGGGGATTATCCTGTGCTCTCCCAGTAACCAGGTATCAAA ATAATGAATCTGAGGATTCTGAAGAATGGAAGGGTCATTTGGCTCAGGCACCAACACCACCCTTGGCAAA TGAAGACCCCAGTGACTGCACTCAGTCAGAGGAGGGCCTGGGCTCTGATGATCATCAATACATTTATAGG CTAGCTGGTGGCTTCTCCAGGAGCACAGGAAAAGGAGGAGATGATAAAGATGACGATGAAGATGACAGTG GAGATGACACCTTTGGTGACGATGACAGTGGCCCAGGGCCCAAAGACAGACAAGAAGGAGGAAACTCCAG ACTGGGAAGTGATGAGGACTCTGATGACACCATACAAGCCAGTGAAGAGAGTGCCCCACAAGGGCAAGAC AGTGCCCAAGATACCACCAGTGAGAGCAGGGAACTTGACAATGAGGACCGGGTGGACAGCAAGCCTGAGG GAGGTGACTCCACTCAAGAGAGTGAGAGTGAAGAGCACTGGGTGGGAGGTGGCAGTGATGGGGAGAGCAG CCATGGAGACGGCTCCGAGTTGGACGATGAGGGAATGCAGAGTGATGACCCAGAGAGCATCAGGAGTGAA AGGGGAAACTCCAGAATGAACAGTGCAGGCATGAAATCAAAAGAATCTGGAGAAAACAGTGAGCAAGCAA ACACTCAAGATTCAGGTGGCAGCCAATTGCTGGAGCATCCCAGTAGGAAAATTTTTAGGAAGTCTCGCAT CTCAGAGGAAGATGACAGAAGCGAGCTTGATGACAACAACACAATGGAAGAAGTCAAGAGTGACTCTACA GAAAACAGCAACTCCAGAGACACTGGCCTCAGCCAACCCAGGAGAGACAGCAAGGGTGACTCTCAAGAAG ACAGCAAGGAGAATCTGTCCCAGGAAGAGAGCCAAAACGTAGATGGTCCCAGCAGTGAGTCCAGCCAAGA GGCCAACCTGTCATCTCAAGAGAACAGCAGTGAGTCTCAGGAAGAGGTGGTGAGTGAGTCCAGGGGAGAT AACCCCGACCCCACAACTAGTTATGTAGAAGACCAGGAAGACAGTGACTCCAGCGAGGAGGACAGCTCGC ACACACTCTCCCACTCAAAAAGTGAATCCAGAGAGGAGCAAGCAGACAGCGAATCCAGTGAGAGCCTCAA CTTCTCAGAGGAAAGCCCGGAGTCCCCTGAGGATGAGAACAGCTCCAGCCAGGAGGGCCTCCAGTCTCAC AGCAGCTCAGCAGAGAGTCAGAGCGAGGAAAGCCATTCTGAGGAAGACGACAGTGACTCTCAAGACAGCA GCAGATCCAAAGAAGATAGCAACTCCACGGAGAGCAAATCAAGCAGTGAGGAAGATGGCCAGTTGAAAAA CATTGAGATAGAGAGCCGGAAATTAACAGTTGATGCCTATCACAACAAACCCATTGGGGACCAAGATGAC AATGACTGCCAAGACGGCTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222208 representing NM_001079911
Red=Cloning site Green=Tags(s) MKISILLMFLWGLSCALPVTRYQNNESEDSEEWKGHLAQAPTPPLANEDPSDCTQSEEGLGSDDHQYIYR LAGGFSRSTGKGGDDKDDDEDDSGDDTFGDDDSGPGPKDRQEGGNSRLGSDEDSDDTIQASEESAPQGQD SAQDTTSESRELDNEDRVDSKPEGGDSTQESESEEHWVGGGSDGESSHGDGSELDDEGMQSDDPESIRSE RGNSRMNSAGMKSKESGENSEQANTQDSGGSQLLEHPSRKIFRKSRISEEDDRSELDDNNTMEEVKSDST ENSNSRDTGLSQPRRDSKGDSQEDSKENLSQEESQNVDGPSSESSQEANLSSQENSSESQEEVVSESRGD NPDPTTSYVEDQEDSDSSEEDSSHTLSHSKSESREEQADSESSESLNFSEESPESPEDENSSSQEGLQSH SSSAESQSEESHSEEDDSDSQDSSRSKEDSNSTESKSSSEEDGQLKNIEIESRKLTVDAYHNKPIGDQDD NDCQDGY TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001079911 |
ORF Size | 1491 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001079911.1, NP_001073380.1 |
RefSeq Size | 2631 bp |
RefSeq ORF | 1494 bp |
Locus ID | 1758 |
UniProt ID | Q13316 |
Protein Families | Secreted Protein |
Gene Summary | Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222208 | DMP1 (Myc-DDK-tagged)-Human dentin matrix acidic phosphoprotein 1 (DMP1), transcript variant 2 |
CNY 3,656.00 |
|
RC222208L3 | Lenti ORF clone of Human dentin matrix acidic phosphoprotein 1 (DMP1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC222208L4 | Lenti ORF clone of Human dentin matrix acidic phosphoprotein 1 (DMP1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC315785 | DMP1 (untagged)-Human dentin matrix acidic phosphoprotein 1 (DMP1), transcript variant 2 |
CNY 7,980.00 |