United States
Germany
Japan
United Kingdom
China
Phospholipase c eta 1 (PLCH1) (NM_001130961) Human Tagged ORF Clone
CAT＃: RG226295
- TrueORF®
PLCH1 (tGFP-tagged) - Human phospholipase C, eta 1 (PLCH1), transcript variant 3
ORF Plasmid: DDK
"NM_001130961" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 11,020.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PLC-L3; PLC eta 1; PLCL3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226295 representing NM_001130961
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACCTTGAAGTGTATAAAAACTTAAGTCCAGAAAAAGTTGAAAGATGCATGAGTGTGATGCAGT CCGGGACACAGATGATCAAGCTGAAACGTGGAACCAAAGGGCTTGTCCGCCTCTTTTACCTGGATGAGCA CCGGACACGCCTCCGATGGCGACCCTCTAGGAAGAGTGAGAAGGCAAAAATACTTATTGATTCCATTTAC AAAGTGACTGAGGGCCGGCAGTCTGAAATATTCCACAGACAAGCTGAGGGGAACTTCGACCCCAGCTGCT GCTTCACCATCTACCATGGCAACCACATGGAGTCCCTGGACCTCATCACCTCCAACCCCGAGGAGGCCCG CACCTGGATCACAGGCCTCAAGTACCTGATGGCTGGCATCAGTGATGAAGACTCCCTTGCCAAAAGGCAG AGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAAGCTGATAAGAATGGTGACGGCTTGCTGAATA TTGAAGAGATACATCAGCTGATGCATAAACTGAATGTTAATCTGCCCCGAAGAAAAGTCAGACAAATGTT TCAGGAAGCCGACACAGATGAGAATCAGGGAACTTTGACATTTGAAGAGTTCTGTGTTTTTTACAAAATG ATGTCTTTGAGACGAGACCTTTATTTGTTACTTTTGAGCTACAGTGACAAGAAAGATCACCTAACTGTGG AAGAACTGGCTCAGTTTTTGAAGGTGGAGCAAAAGATGAATAATGTGACAACGGACTATTGTCTTGACAT CATAAAGAAGTTTGAAGTTTCAGAAGAAAATAAGGTGAAAAATGTTCTTGGCATAGAAGGCTTCACGAAC TTCATGCGTAGTCCTGCCTGTGACATATTTAACCCATTGCACCATGAAGTGTACCAAGACATGGATCAGC CCCTCTGCAACTACTACATTGCTTCCTCTCACAATACATACCTGACTGGAGACCAGCTCCTTTCTCAGTC CAAAGTGGATATGTATGCACGGGTGCTGCAAGAGGGCTGTCGCTGTGTGGAAGTTGACTGTTGGGATGGC CCAGATGGAGAGCCAGTAGTACATCATGGTTACACTCTCACTTCAAAAATTCTCTTCAGAGATGTTGTGG AGACCATCAACAAGCATGCCTTTGTGAAGAATGAGTTTCCTGTTATATTGTCTATCGAGAATCACTGCAG TATCCAGCAGCAAAGGAAGATTGCTCAGTACCTGAAAGGAATATTCGGAGACAAACTGGACCTGTCATCT GTTGATACAGGGGAGTGCAAGCAGCTTCCAAGCCCTCAAAGTTTGAAAGGCAAAATTCTAGTGAAGGGTA AGAAGTTGCCTTATCACCTTGGGGATGATGCAGAGGAAGGGGAAGTTTCTGATGAGGACAGTGCAGATGA AATTGAAGACGAGTGCAAATTCAAGCTCCATTATAGTAATGGGACCACTGAGCATCAGGTGGAATCTTTC ATAAGGAAAAAACTGGAGTCACTGTTAAAAGAATCTCAAATTCGAGATAAAGAAGATCCTGATAGTTTCA CAGTGCGGGCACTACTGAAGGCCACGCATGAAGGCTTAAATGCACACCTGAAGCAGAGTCCAGATGTAAA GGAAAGTGGAAAGAAATCACATGGACGATCCCTCATGACCAACTTTGGAAAACATAAGAAAACTACAAAA TCACGGTCTAAATCTTACAGTACTGATGATGAGGAAGACACACAGCAGAGTACTGGCAAGGAGGGTGGCC AGCTGTACAGATTGGGTCGCCGAAGGAAAACCATGAAGCTCTGCCGAGAACTCTCTGATTTGGTTGTGTA CACAAACTCCGTGGCCGCTCAGGACATTGTGGATGACGGAACCACAGGAAATGTGTTATCATTCAGTGAA ACAAGAGCACATCAGGTTGTTCAGCAAAAATCAGAGCAGTTCATGATTTATAATCAAAAGCAACTCACGA GGATTTACCCCTCTGCCTACCGCATTGATTCCAGTAACTTCAACCCTCTCCCCTACTGGAACGCAGGCTG CCAGCTAGTGGCACTGAATTATCAATCTGAAGGACGAATGATGCAGTTAAACCGAGCCAAATTCAAGGCA AATGGCAATTGTGGCTATGTCCTCAAACCCCAGCAAATGTGCAAAGGTACTTTCAACCCTTTCTCTGGTG ACCCTCTTCCTGCCAACCCCAAAAAGCAGCTCATCCTGAAAGTTATCAGTGGACAGCAACTCCCCAAACC TCCAGACTCCATGTTTGGAGATCGAGGCGAGATCATTGACCCTTTTGTTGAAGTTGAAATTATTGGATTG CCAGTAGATTGTTGTAAAGATCAAACCCGTGTGGTAGATGACAATGGATTTAACCCTGTGTGGGAAGAAA CACTGACATTTACAGTACACATGCCAGAAATAGCTTTGGTTCGGTTCCTTGTGTGGGATCACGATCCCAT TGGACGAGACTTTGTTGGACAAAGAACTGTGACCTTCAGCAGCTTAGTGCCTGGCTACCGGCATGTCTAT TTGGAAGGACTGACAGAAGCATCCATATTTGTACACATAACCATCAATGAAATCTATGGAAAGTGGAGCC CTTTAATACTCAACCCCAGTTATACTATATTGCACTTTCTAGGAGCTACAAAGAACAGACAACTCCAGGG TCTGAAGGGACTGTTCAATAAGAATCCTAGGCACAGTTCTTCAGAAAACAATTCCCATTATGTACGGAAG CGATCCATTGGAGATAGAATTCTGCGACGCACAGCTAGCGCCCCAGCCAAAGGCAGGAAAAAGAGCAAAA TGGGCTTCCAAGAAATGGTGGAGATAAAGGATTCTGTGTCCGAGGCCACAAGAGATCAAGATGGCGTGCT GAGGAGGACCACACGCAGTTTGCAAGCACGCCCTGTCTCTATGCCTGTTGACAGAAACCTTCTGGGAGCT TTGTCGCTGCCTGTATCTGAAACAGCAAAAGACATTGAAGGAAAAGAAAACTCTCTAGTACAAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226295 representing NM_001130961
Red=Cloning site Green=Tags(s) MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIY KVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQ RTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKM MSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTN FMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDG PDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSS VDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESF IRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTK SRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSE TRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKA NGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGL PVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVY LEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRK RSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGA LSLPVSETAKDIEGKENSLVQI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130961 |
| ORF Size | 3006 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130961.2 |
| RefSeq Size | 6473 bp |
| RefSeq ORF | 3009 bp |
| Locus ID | 23007 |
| UniProt ID | Q4KWH8 |
| Protein Families | Druggable Genome |
| Gene Summary | PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226295 | PLCH1 (Myc-DDK-tagged)-Human phospholipase C, eta 1 (PLCH1), transcript variant 3 |
CNY 9,980.00 |
|
| RC226295L3 | Lenti-ORF clone of PLCH1 (Myc-DDK-tagged)-Human phospholipase C, eta 1 (PLCH1), transcript variant 3 |
CNY 11,880.00 |
|
| RC226295L4 | Lenti-ORF clone of PLCH1 (mGFP-tagged)-Human phospholipase C, eta 1 (PLCH1), transcript variant 3 |
CNY 11,880.00 |
|
| SC326312 | PLCH1 (untagged)-Human phospholipase C, eta 1 (PLCH1), transcript variant 3 |
CNY 19,290.00 |
其它Phospholipase c eta 1产品
