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Angiomotin (AMOT) (NM_001113490) Human Tagged ORF Clone
CAT＃: RG226319
- TrueORF®
AMOT (tGFP-tagged) - Human angiomotin (AMOT), transcript variant 1
ORF Plasmid: DDK
"NM_001113490" in other vectors (6)
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CNY 13,672.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226319 representing NM_001113490
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAATTCTGAAGAACAGCCAAGTGGAGGGACCACGGTATTGCAGCGTTTGCTACAAGAGCAGCTTC GCTATGGCAATCCTAGTGAGAATCGCAGCCTGCTTGCCATACACCAGCAAGCCACAGGGAATGGCCCTCC TTTCCCCAGTGGCAGTGGGAACCCGGGCCCTCAGAGTGATGTGTTGAGTCCCCAAGACCACCACCAACAG CTTGTGGCTCATGCTGCTCGACAAGAACCCCAGGGGCAGGAAATCCAGTCAGAAAACCTCATCATGGAGA AGCAGCTGTCTCCTCGAATGCAAAATAATGAAGAACTCCCGACCTATGAAGAAGCCAAGGTCCAGTCCCA GTACTTTCGGGGCCAACAGCATGCCAGTGTTGGAGCTGCCTTCTATGTCACTGGAGTCACCAACCAGAAG ATGAGGACTGAGGGACGCCCATCAGTTCAGCGGCTCAATCCTGGAAAGATGCACCAAGATGAGGGACTCA GAGACCTTAAGCAAGGGCATGTCCGTTCCTTGAGTGAACGACTAATGCAGATGTCACTGGCCACCAGTGG AGTTAAGGCCCATCCACCTGTTACCAGTGCTCCCCTCTCCCCACCACAACCCAATGACCTCTACAAGAAT CCCACAAGTTCCAGTGAATTCTACAAGGCCCAAGGGCCACTTCCTAACCAGCATAGCCTGAAGGGCATGG AACACCGAGGCCCCCCACCAGAATATCCCTTCAAGGGCATGCCACCCCAATCTGTAGTGTGCAAGCCCCA AGAGCCAGGGCACTTCTATAGTGAGCATCGCCTGAACCAGCCAGGGAGAACAGAGGGGCAACTGATGAGG TATCAGCATCCCCCTGAGTATGGAGCAGCCAGGCCAGCGCAGGACATCTCATTGCCATTGTCAGCCAGGA ACTCGCAGCCTCACAGCCCTACTTCTTCTCTGACCTCTGGGGGGTCCTTGCCCTTGCTACAATCTCCACC ATCCACTAGATTGTCTCCTGCCCGACACCCCTTGGTCCCAAACCAGGGAGACCATTCAGCTCACCTGCCT AGGCCGCAGCAGCATTTCCTTCCTAATCAGGCTCACCAGGGGGATCATTACCGTCTCTCCCAACCTGGCC TGAGTCAGCAGCAGCAGCAACAGCAGCAGCAGCACCATCATCACCATCACCACCAACAACAGCAGCAGCA GCAGCCACAGCAGCAGCCAGGAGAAGCCTATTCAGCTATGCCTCGGGCTCAGCCATCCTCTGCTTCTTAT CAGCCAGTGCCAGCAGACCCTTTTGCCATTGTTTCCAGAGCCCAGCAGATGGTTGAGATCCTCTCAGACG AGAACCGGAACTTGAGGCAAGAGTTGGAAGGATGCTATGAGAAGGTGGCAAGACTGCAGAAGGTGGAGAC AGAAATCCAGCGCGTCTCGGAGGCATATGAGAACCTCGTGAAGTCATCCTCCAAAAGAGAGGCCCTAGAG AAAGCCATGAGAAACAAGCTAGAGGGCGAGATTCGGAGGATGCATGATTTCAACAGGGATCTGAGAGAGC GTCTAGAGACTGCCAACAAGCAGCTTGCAGAGAAGGAATATGAGGGGTCAGAGGACACCAGAAAAACCAT CTCGCAGCTCTTTGCAAAAAATAAAGAAAGCCAGCGTGAGAAGGAGAAGCTGGAAGCGGAGCTGGCCACT GCCCGTTCTACCAATGAGGACCAAAGACGACACATCGAAATCCGAGATCAGGCCCTGAGTAATGCCCAGG CCAAGGTGGTAAAGCTGGAAGAAGAGCTGAAAAAGAAGCAAGTGTACGTTGACAAGGTGGAGAAGATGCA GCAGGCCCTTGTACAGCTCCAGGCAGCATGTGAAAAACGTGAGCAGCTAGAGCACCGTCTCCGGACACGA CTGGAGAGGGAACTGGAATCCCTGAGAATCCAGCAGCGTCAGGGCAACTGTCAGCCCACCAACGTTTCAG AATACAATGCTGCTGCACTGATGGAGCTCCTTCGGGAGAAAGAGGAGAGGATTCTGGCTCTGGAAGCTGA TATGACAAAGTGGGAGCAGAAATATTTGGAGGAGAATGTGATGAGACATTTTGCTCTGGATGCTGCTGCA ACTGTGGCTGCTCAGAGGGACACAACAGTCATCAGTCACTCTCCTAACACCAGCTATGACACAGCTCTAG AAGCTCGCATCCAGAAAGAGGAGGAAGAAATCTTGATGGCCAATAAGCGTTGCCTTGACATGGAGGGCAG GATTAAGACCCTCCATGCCCAGATTATTGAGAAGGATGCCATGATCAAAGTACTCCAGCAGCGTTCCCGG AAGGAGCCGAGCAAGACAGAGCAGCTGTCGTGCATGCGGCCAGCGAAGTCTCTGATGTCCATTTCCAATG CTGGATCAGGCTTGCTCTCCCACTCATCCACCCTGACTGGCTCCCCCATCATGGAAGAAAAGCGAGACGA CAAGAGCTGGAAGGGGAGCCTAGGCATTCTCCTGGGTGGAGACTACCGTGCTGAATATGTCCCTTCCACA CCCTCGCCTGTGCCACCCTCGACTCCCCTGCTCTCGGCTCACTCCAAGACAGGCAGCCGAGACTGCAGTA CCCAAACTGAACGTGGGACGGAATCGAACAAAACTGCAGCTGTTGCTCCCATCTCTGTTCCTGCTCCAGT TGCTGCTGCCGCCACTGCTGCCGCCATCACTGCCACTGCTGCCACCATCACCACCACCATGGTAGCTGCT GCTCCAGTTGCTGTTGCTGCTGCTGCTGCTCCAGCTGCTGCTGCTGCCCCGTCTCCAGCCACTGCCGCTG CTACTGCTGCTGCTGTTTCTCCAGCTGCTGCTGGTCAGATTCCAGCTGCTGCCTCTGTTGCCTCAGCTGC TGCCGTTGCTCCTTCTGCTGCTGCTGCTGCTGCTGTTCAGGTTGCTCCAGCTGCTCCGGCTCCAGTTCCA GCTCCGGCTCTGGTTCCGGTTCCAGCTCCAGCAGCGGCTCAGGCTTCTGCTCCTGCTCAGACTCAGGCAC CAACTTCAGCTCCGGCTGTGGCTCCAACTCCAGCTCCAACTCCAACTCCAGCTGTGGCTCAGGCTGAGGT TCCTGCAAGTCCAGCTACCGGTCCTGGACCACATCGTTTGTCTATACCAAGTTTGACCTGCAATCCAGAC AAAACAGATGGGCCTGTGTTCCACTCCAATACTCTGGAAAGAAAAACTCCCATTCAGATCCTGGGACAAG AGCCTGATGCAGAGATGGTGGAATATCTCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226319 representing NM_001113490
Red=Cloning site Green=Tags(s) MRNSEEQPSGGTTVLQRLLQEQLRYGNPSENRSLLAIHQQATGNGPPFPSGSGNPGPQSDVLSPQDHHQQ LVAHAARQEPQGQEIQSENLIMEKQLSPRMQNNEELPTYEEAKVQSQYFRGQQHASVGAAFYVTGVTNQK MRTEGRPSVQRLNPGKMHQDEGLRDLKQGHVRSLSERLMQMSLATSGVKAHPPVTSAPLSPPQPNDLYKN PTSSSEFYKAQGPLPNQHSLKGMEHRGPPPEYPFKGMPPQSVVCKPQEPGHFYSEHRLNQPGRTEGQLMR YQHPPEYGAARPAQDISLPLSARNSQPHSPTSSLTSGGSLPLLQSPPSTRLSPARHPLVPNQGDHSAHLP RPQQHFLPNQAHQGDHYRLSQPGLSQQQQQQQQQHHHHHHHQQQQQQQPQQQPGEAYSAMPRAQPSSASY QPVPADPFAIVSRAQQMVEILSDENRNLRQELEGCYEKVARLQKVETEIQRVSEAYENLVKSSSKREALE KAMRNKLEGEIRRMHDFNRDLRERLETANKQLAEKEYEGSEDTRKTISQLFAKNKESQREKEKLEAELAT ARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVEKMQQALVQLQAACEKREQLEHRLRTR LERELESLRIQQRQGNCQPTNVSEYNAAALMELLREKEERILALEADMTKWEQKYLEENVMRHFALDAAA TVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDMEGRIKTLHAQIIEKDAMIKVLQQRSR KEPSKTEQLSCMRPAKSLMSISNAGSGLLSHSSTLTGSPIMEEKRDDKSWKGSLGILLGGDYRAEYVPST PSPVPPSTPLLSAHSKTGSRDCSTQTERGTESNKTAAVAPISVPAPVAAAATAAAITATAATITTTMVAA APVAVAAAAAPAAAAAPSPATAAATAAAVSPAAAGQIPAAASVASAAAVAPSAAAAAAVQVAPAAPAPVP APALVPVPAPAAAQASAPAQTQAPTSAPAVAPTPAPTPTPAVAQAEVPASPATGPGPHRLSIPSLTCNPD KTDGPVFHSNTLERKTPIQILGQEPDAEMVEYLI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001113490 |
| ORF Size | 3252 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001113490.1, NP_001106962.1 |
| RefSeq Size | 6945 bp |
| RefSeq ORF | 3255 bp |
| Locus ID | 154796 |
| UniProt ID | Q4VCS5 |
| Gene Summary | This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226319 | AMOT (Myc-DDK-tagged)-Human angiomotin (AMOT), transcript variant 1 |
CNY 12,072.00 |
|
| RC226319L1 | Lenti-ORF clone of AMOT (Myc-DDK-tagged)-Human angiomotin (AMOT), transcript variant 1 |
CNY 14,472.00 |
|
| RC226319L2 | Lenti-ORF clone of AMOT (mGFP-tagged)-Human angiomotin (AMOT), transcript variant 1 |
CNY 12,730.00 |
|
| RC226319L3 | Lenti-ORF clone of AMOT (Myc-DDK-tagged)-Human angiomotin (AMOT), transcript variant 1 |
CNY 12,730.00 |
|
| RC226319L4 | Lenti-ORF clone of AMOT (mGFP-tagged)-Human angiomotin (AMOT), transcript variant 1 |
CNY 14,472.00 |
|
| SC319022 | AMOT (untagged)-Human angiomotin (AMOT), transcript variant 1 |
CNY 20,810.00 |
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