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VPS52 (NM_001289174) Human Tagged ORF Clone
CAT＃: RG239228
- TrueORF®
VPS52 (tGFP-tagged) - Human vacuolar protein sorting 52 homolog (S. cerevisiae) (VPS52), transcript variant 2
ORF Plasmid: DDK
"NM_001289174" in other vectors (2)
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CNY 4,940.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARE1; dJ1033B10.5; SAC2; SACM2L |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239228 representing NM_001289174.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATTCATCCTGGATGAAGTGGATGGGTGTAGATCTCCGTCACTATTCAAAGCAAGTTGAGCTGGAG CTACAGCAGATTGAACAGAAATCCATTCGGGATTATATTCAAGAGAGTGAGAATATAGCATCTCTACAC AACCAGATCACAGCCTGTGATGCTGTCCTGGAGCGAATGGAGCAGATGTTGGGAGCTTTTCAGAGTGAC CTCAGCTCCATCAGCTCTGAGATCCGGACACTGCAGGAACAGTCAGGAGCCATGAACATTCGACTTCGA AATCGCCAGGCAGTTCGGGGGAAACTTGGGGAGCTTGTTGATGGTCTGGTGGTGCCTTCTGCTCTGGTC ACGGCAATTCTGGAGGCTCCAGTGACAGAGCCCAGGTTCTTGGAGCAGCTACAGGAGCTGGATGCCAAG GCAGCCGCAGTCAGAGAGCAGGAAGCTAGAGGCACAGCAGCCTGCGCAGATGTCAGAGGCGTGCTCGAT CGGCTCCGGGTCAAGGCAGTGACGAAGATCCGAGAGTTTATCCTCCAGAAGATTTATTCCTTCAGGAAA CCCATGACCAACTATCAGATCCCCCAGACGGCCCTGCTGAAGTACAGGTTCTTCTATCAGTTTCTGCTG GGCAATGAACGAGCAACAGCAAAGGAGATCAGGGATGAATATGTGGAGACGCTGAGCAAGATTTACCTG TCTTACTACCGCTCTTACCTGGGGCGGCTCATGAAGGTGCAGTATGAGGAAGTCGCTGAGAAAGATGAT CTAATGGGTGTGGAAGATACAGCAAAGAAAGGATTCTTCTCAAAGCCATCGCTCCGCAGCAGGAACACC ATTTTCACCCTAGGAACCCGCGGCTCTGTCATCTCCCCCACTGAACTTGAGGCCCCCATCCTGGTGCCT CACACAGCGCAGCGCGGAGAGCAGAGGTATCCATTTGAGGCCCTCTTCCGCAGCCAGCACTACGCCCTC CTAGACAATTCCTGCCGCGAATACCTTTTCATCTGTGAATTTTTTGTTGTGTCTGGCCCAGCTGCACAC GACCTGTTCCATGCTGTCATGGGCCGTACACTCAGCATGACCCTGAAACACCTGGATTCTTATCTAGCT GACTGCTACGATGCCATTGCTGTTTTTCTCTGTATCCACATTGTTCTCCGGTTCCGTAACATTGCAGCA AAGAGGGATGTTCCTGCCCTGGACAGGTACTGGGAACAGGTGCTTGCCTTGCTATGGCCACGGTTTGAA CTGATCCTGGAGATGAATGTTCAGAGCGTCCGAAGCACTGACCCCCAGCGCCTAGGGGGGTTGGATACT CGGCCCCACTATATCACACGCCGCTATGCAGAGTTCTCCTCCGCTCTTGTCAGTATCAACCAGACAATT CCTAATGAACGGACCATGCAATTGCTGGGACAGCTGCAGGTGGAGGTGGAGAATTTTGTCCTCCGAGTG GCAGCTGAGTTCTCCTCAAGGAAGGAGCAGCTTGTGTTTCTGATCAACAACTATGACATGATGCTGGGT GTGCTGATGGAGCGGGCTGCAGATGACAGCAAAGAGGTTGAGAGCTTCCAGCAGCTGCTCAATGCTCGG ACACAGGAATTCATTGAAGAGTTGCTGTCTCCCCCTTTTGGGGGTTTAGTGGCATTTGTGAAGGAGGCT GAGGCTTTGATTGAGCGTGGACAGGCTGAGCGACTTCGAGGGGAAGAAGCCCGGGTAACTCAGCTGATC CGTGGCTTTGGTAGTTCCTGGAAATCATCAGTGGAATCTCTGAGTCAGGATGTAATGCGGAGTTTCACC AACTTCAGAAATGGCACCAGTATCATTCAGGGAGCGCTGACCCAGCTGATCCAGCTCTATCATCGCTTC CACCGGGTGCTGTCCCAGCCGCAGCTCCGAGCCCTCCCTGCCCGGGCTGAGCTCATCAACATTCACCAC CTTATGGTGGAGCTCAAGAAGCATAAGCCCAACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239228
Blue=ORF Red=Cloning site Green=Tag(s) MNSSWMKWMGVDLRHYSKQVELELQQIEQKSIRDYIQESENIASLHNQITACDAVLERMEQMLGAFQSD LSSISSEIRTLQEQSGAMNIRLRNRQAVRGKLGELVDGLVVPSALVTAILEAPVTEPRFLEQLQELDAK AAAVREQEARGTAACADVRGVLDRLRVKAVTKIREFILQKIYSFRKPMTNYQIPQTALLKYRFFYQFLL GNERATAKEIRDEYVETLSKIYLSYYRSYLGRLMKVQYEEVAEKDDLMGVEDTAKKGFFSKPSLRSRNT IFTLGTRGSVISPTELEAPILVPHTAQRGEQRYPFEALFRSQHYALLDNSCREYLFICEFFVVSGPAAH DLFHAVMGRTLSMTLKHLDSYLADCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFE LILEMNVQSVRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTMQLLGQLQVEVENFVLRV AAEFSSRKEQLVFLINNYDMMLGVLMERAADDSKEVESFQQLLNARTQEFIEELLSPPFGGLVAFVKEA EALIERGQAERLRGEEARVTQLIRGFGSSWKSSVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRF HRVLSQPQLRALPARAELINIHHLMVELKKHKPNF TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001289174 |
| ORF Size | 1968 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001289174.2 |
| RefSeq Size | 3442 bp |
| RefSeq ORF | 1971 bp |
| Locus ID | 6293 |
| MW | 75.8 kDa |
| Gene Summary | This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Documents
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