VPS52 (NM_001289174) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARE1; dJ1033B10.5; SAC2; SACM2L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337122 representing NM_001289174. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATTCATCCTGGATGAAGTGGATGGGTGTAGATCTCCGTCACTATTCAAAGCAAGTTGAGCTGGAG CTACAGCAGATTGAACAGAAATCCATTCGGGATTATATTCAAGAGAGTGAGAATATAGCATCTCTACAC AACCAGATCACAGCCTGTGATGCTGTCCTGGAGCGAATGGAGCAGATGTTGGGAGCTTTTCAGAGTGAC CTCAGCTCCATCAGCTCTGAGATCCGGACACTGCAGGAACAGTCAGGAGCCATGAACATTCGACTTCGA AATCGCCAGGCAGTTCGGGGGAAACTTGGGGAGCTTGTTGATGGTCTGGTGGTGCCTTCTGCTCTGGTC ACGGCAATTCTGGAGGCTCCAGTGACAGAGCCCAGGTTCTTGGAGCAGCTACAGGAGCTGGATGCCAAG GCAGCCGCAGTCAGAGAGCAGGAAGCTAGAGGCACAGCAGCCTGCGCAGATGTCAGAGGCGTGCTCGAT CGGCTCCGGGTCAAGGCAGTGACGAAGATCCGAGAGTTTATCCTCCAGAAGATTTATTCCTTCAGGAAA CCCATGACCAACTATCAGATCCCCCAGACGGCCCTGCTGAAGTACAGGTTCTTCTATCAGTTTCTGCTG GGCAATGAACGAGCAACAGCAAAGGAGATCAGGGATGAATATGTGGAGACGCTGAGCAAGATTTACCTG TCTTACTACCGCTCTTACCTGGGGCGGCTCATGAAGGTGCAGTATGAGGAAGTCGCTGAGAAAGATGAT CTAATGGGTGTGGAAGATACAGCAAAGAAAGGATTCTTCTCAAAGCCATCGCTCCGCAGCAGGAACACC ATTTTCACCCTAGGAACCCGCGGCTCTGTCATCTCCCCCACTGAACTTGAGGCCCCCATCCTGGTGCCT CACACAGCGCAGCGCGGAGAGCAGAGGTATCCATTTGAGGCCCTCTTCCGCAGCCAGCACTACGCCCTC CTAGACAATTCCTGCCGCGAATACCTTTTCATCTGTGAATTTTTTGTTGTGTCTGGCCCAGCTGCACAC GACCTGTTCCATGCTGTCATGGGCCGTACACTCAGCATGACCCTGAAACACCTGGATTCTTATCTAGCT GACTGCTACGATGCCATTGCTGTTTTTCTCTGTATCCACATTGTTCTCCGGTTCCGTAACATTGCAGCA AAGAGGGATGTTCCTGCCCTGGACAGGTACTGGGAACAGGTGCTTGCCTTGCTATGGCCACGGTTTGAA CTGATCCTGGAGATGAATGTTCAGAGCGTCCGAAGCACTGACCCCCAGCGCCTAGGGGGGTTGGATACT CGGCCCCACTATATCACACGCCGCTATGCAGAGTTCTCCTCCGCTCTTGTCAGTATCAACCAGACAATT CCTAATGAACGGACCATGCAATTGCTGGGACAGCTGCAGGTGGAGGTGGAGAATTTTGTCCTCCGAGTG GCAGCTGAGTTCTCCTCAAGGAAGGAGCAGCTTGTGTTTCTGATCAACAACTATGACATGATGCTGGGT GTGCTGATGGAGCGGGCTGCAGATGACAGCAAAGAGGTTGAGAGCTTCCAGCAGCTGCTCAATGCTCGG ACACAGGAATTCATTGAAGAGTTGCTGTCTCCCCCTTTTGGGGGTTTAGTGGCATTTGTGAAGGAGGCT GAGGCTTTGATTGAGCGTGGACAGGCTGAGCGACTTCGAGGGGAAGAAGCCCGGGTAACTCAGCTGATC CGTGGCTTTGGTAGTTCCTGGAAATCATCAGTGGAATCTCTGAGTCAGGATGTAATGCGGAGTTTCACC AACTTCAGAAATGGCACCAGTATCATTCAGGGAGCGCTGACCCAGCTGATCCAGCTCTATCATCGCTTC CACCGGGTGCTGTCCCAGCCGCAGCTCCGAGCCCTCCCTGCCCGGGCTGAGCTCATCAACATTCACCAC CTTATGGTGGAGCTCAAGAAGCATAAGCCCAACTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289174
Insert Size	1971 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289174.1
RefSeq Size	3442 bp
RefSeq ORF	1971 bp
Locus ID	6293
MW	75.4 kDa
Gene Summary	This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) differs in the 5' UTR, initiates translation at an alternate start codon and lacks an alternate exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1.

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