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Sptlc1 (NM_001108406) Rat Untagged Clone
CAT＃: RN201484
Sptlc1 (untagged ORF) - Rat serine palmitoyltransferase, long chain base subunit 1 (Sptlc1), (10 ug)
CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Lcb1; RGD1306617; Spt1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201484 representing NM_001108406
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGTGGCGGAGCAGTGGGTGCTGGTGGAGATGGTGCAGGCGCTGTACGAGGCTCCAGCATACC ATCTAATTCTGGAAGGAATCCTCATACTTTGGATAATCAGACTCGTTTTCTCTAAAACTTACAAGTTGCA GGAGCGTTCTGACCTTACAGCCAAGGAAAAGGAAGAACTGATTGAAGAATGGCAGCCAGAGCCCCTCGTC CCCCCGGTCTCCAGGAACCATCCTGCTCTCAACTACAACATCGTCTCCGGCCCTCCAACCCATAACATCG TGGTGAACGGAAAAGAGTGTGTCAACTTTGCCTCCTTTAATTTCCTCGGGTTGCTGGCCAACCCTCGGGT TAAGGCTGCGGCTTTTGCATCATTAAAGAAGTACGGCGTGGGTACCTGTGGTCCCAGAGGGTTTTATGGC ACATTTGATGTCCATCTGGATTTGGAAGAGCGCCTGGCAAAGTTTATGAAGACCGAAGAAGCTATCATTT ACTCCTATGGCTTCTCCACCATAGCCAGTGCGATTCCTGCGTACTCTAAGAGAGGGGACATTGTTTTTGT GGACAGTGCAGCCTGCTTTGCTATCCAGAAAGGACTACAGGCATCACGCAGTGACATTAAGTTATTCAAG CACAATGACGTAGCTGACCTGGAGCGACTGCTAAAAGAACAAGAGATTGAAGATCAAAAGAATCCTCGAA AGGCCCGTGTGACTCGGCGATTCATCGTCGCGGAAGGATTGTATATGAACACTGGAACCATCTGCCCTCT TCCAGAACTGGTGAGGTTAAAGTATAAATACAAAGCAAGGATCTTCCTGGAGGAGAGCCTGTCGTTCGGA GTCCTTGGGGAGCATGGGCGAGGAGTCACCGAGCACTATGGGATCAGTATTGACGACATCGACCTTATCA GTGCTAACATGGAGAATGCGCTCGCTTCTGTTGGGGGCTTCTGCTGTGGCCGCTCTTTCGTGGTTGACCA TCAGCGGCTCTCTGGCCAAGGATACTGCTTTTCAGCTTCACTGCCCCCCCTGCTAGCCGCTGCCGCCATT GAGGCCCTCAACATCATGGAGGAGAACCCAGGGATTTTTGCAGTTTTAAAGAAAAAGTGCCAGACCATCC ACAAGTCCCTACAAGGGGTTTCCGGTTTAAAAGTGGTGGGGGAGTCCCTTTGCCCAGCGCTTCACCTCCA GCTGGAAGAGAGCACGGGCTCTCGGGAGAGAGATATGAAGCTGCTTCAGGAGATTGTAGAGCAATGCATG AATAAGGGCATCGCATTGACTCAGGCACGCTACTTGGACAAGGAAGAGAAGTGCCTTCCTCCTCCAAGCA TCAGGGTTGTGGTCACCGTGGAGCAGACAGACGAAGAGCTGCAGAGGGCTGCAGCCACCATCAGGGAGGC GGCCCAGGCTGTGCTTTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001108406 |
| Insert Size | 1422 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108406.1, NP_001101876.1 |
| RefSeq Size | 3034 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 361213 |
| UniProt ID | D4A2H2 |
| Gene Summary | Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (By similarity). Required for adipocyte cell viability and metabolic homeostasis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201484 | Sptlc1 (Myc-DDK-tagged ORF) - Rat serine palmitoyltransferase, long chain base subunit 1 (Sptlc1), (10 ug) |
CNY 6,180.00 |
|
| RR201484L3 | Lenti ORF clone of Sptlc1 (Myc-DDK-tagged ORF) - Rat serine palmitoyltransferase, long chain base subunit 1 (Sptlc1), (10 ug) |
CNY 7,980.00 |
|
| RR201484L4 | Lenti ORF clone of Sptlc1 (mGFP-tagged ORF) - Rat serine palmitoyltransferase, long chain base subunit 1 (Sptlc1), (10 ug) |
CNY 8,740.00 |
