Prkci (NM_032059) Rat Untagged Clone

CAT#: RN205182

Prkci (untagged ORF) - Rat protein kinase C, iota (Prkci), (10 ug)



  "NM_032059" in other vectors (3)

CNY 7,700.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Pkcl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN205182 representing NM_032059
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGACCCAGAGGGACAGCAGCACCATGTCTCACACGGTCGCGTGCGGCGGCGGCGGGGACCATTCCC
ACCAGGTCCGGGTGAAAGCCTACTACCGCGGGGATATTATGATAACACACTTCGAGCCTTCCATCTCCTT
TGAAGGACTTTGCAGTGAGGTTCGAGATATGTGTTCTTTTGACAATGAGCAGCCATTCACCATGAAATGG
ATAGATGAGGAAGGAGACCCGTGCACAGTGTCTTCTCAGTTGGAGTTGGAAGAGGCTTTCAGGCTGTATG
AGTTGAACAAGGATTCTGAACTCCTGATCCACGTGTTCCCGTGTGTACCAGAGCGTCCTGGAATGCCTTG
CCCAGGGGAAGACAAGTCCATTTACCGCAGAGGGGCGCGCAGGTGGAGAAAGCTGTATTGTGCAAATGGC
CACACTTTTCAAGCCAAACGCTTTAACAGGCGTGCCCACTGTGCCATCTGCACAGACAGGATCTGGGGAC
TTGGACGACAAGGATATAAATGCATCAACTGCAAACTGCTGGTTCATAAGAAGTGCCACAAGCTTGTCAC
AATTGAGTGTGGGCGGCATTCTTTGCCACCGGAACCCATGATGCCAATGGACCAGTCATCCATGCACCCA
GACCACACACAGACAGTAATTCCATATAATCCTTCAAGTCATGAGAGTTTGGACCAAGTTGGTGAAGAAA
AGGAGGCAATGAACACCAGGGAGAGTGGGAAGGCGTCATCAAGCTTAGGTCTCCAGGATTTCGATTTGCT
TCGAGTTATAGGGAGAGGAAGTTACGCCAAAGTACTGCTGGTTCGATTAAAAAAGACAGATCGCATTTAT
GCAATGAAGGTTGTGAAGAAAGAGCTCGTCAATGACGATGAGGATATTGATTGGGTACAGACAGAAAAGC
ATGTGTTTGAGCAGGCGTCCAATCACCCTTTCCTTGTTGGTCTGCATTCCTGCTTCCAGACAGAAAGCAG
GCTGTTTTTTGTCATAGAATATGTGAATGGAGGGGATCTCATGTTTCATATGCAGCGGCAAAGAAAACTT
CCTGAAGAACATGCCAGGTTTTACTCAGCAGAAATCAGTCTAGCACTAAATTATCTTCATGAGCGAGGGA
TAATTTATAGAGATTTGAAGTTGGACAATGTACTGCTGGACTCTGAAGGACACATCAAACTCACTGACTA
CGGCATGTGTAAGGAAGGATTACGGCCCGGAGATACAACCAGCACCTTCTGTGGGACTCCCAATTACATT
GCTCCTGAGATCTTAAGAGGAGAAGACTATGGCTTCAGCGTTGACTGGTGGGCTCTTGGAGTACTCATGT
TTGAGATGATGGCGGGAAGGTCTCCATTTGATATCGTTGGGAGCTCTGACAATCCTGACCAAAACACAGA
GGATTATCTGTTCCAAGTCATTTTGGAGAAGCAGATTCGCATACCGCGCTCCCTGTCTGTGAAAGCAGCA
AGTGTGCTGAAGAGTTTCCTCAACAAGGACCCAAAGGAACGATTGGGTTGTCACCCTCAAACTGGATTTG
CTGACATCCAAGGACACCCATTCTTCCGTAATGTGGATTGGGACATGATGGAGCAGAAGCAAGTGGTTCC
GCCCTTTAAACCAAACATTTCTGGAGAATTTGGTTTGGATAACTTTGACTCCCAGTTTACCAACGAACCA
GTCCAGCTCACTCCAGATGATGATGACATCGTGAGGAAGATTGATCAGTCTGAATTTGAAGGTTTCGAGT
ATATCAACCCTCTCTTGATGTCTGCAGAAGAGTGTGTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_032059
Insert Size 1791 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032059.1, NP_114448.1
RefSeq Size 4501 bp
RefSeq ORF 1791 bp
Locus ID 84006
UniProt ID F1M7Y5
Gene Summary member of the atypical subgroup of PKCs that do not contain the calcium binding domain and possess only one cysteine-rich region; plays a critical role in controlling cell growth and cell survival; enhances Rab2-mediated vesicle budding [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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