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Prkci (NM_032059) Rat Tagged ORF Clone
CAT＃: RR205182
- TrueORF®
Prkci (Myc-DDK-tagged ORF) - Rat protein kinase C, iota (Prkci), (10 ug)
"NM_032059" in other vectors (3)
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CNY 7,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pkcl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205182 representing NM_032059
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGACCCAGAGGGACAGCAGCACCATGTCTCACACGGTCGCGTGCGGCGGCGGCGGGGACCATTCCC ACCAGGTCCGGGTGAAAGCCTACTACCGCGGGGATATTATGATAACACACTTCGAGCCTTCCATCTCCTT TGAAGGACTTTGCAGTGAGGTTCGAGATATGTGTTCTTTTGACAATGAGCAGCCATTCACCATGAAATGG ATAGATGAGGAAGGAGACCCGTGCACAGTGTCTTCTCAGTTGGAGTTGGAAGAGGCTTTCAGGCTGTATG AGTTGAACAAGGATTCTGAACTCCTGATCCACGTGTTCCCGTGTGTACCAGAGCGTCCTGGAATGCCTTG CCCAGGGGAAGACAAGTCCATTTACCGCAGAGGGGCGCGCAGGTGGAGAAAGCTGTATTGTGCAAATGGC CACACTTTTCAAGCCAAACGCTTTAACAGGCGTGCCCACTGTGCCATCTGCACAGACAGGATCTGGGGAC TTGGACGACAAGGATATAAATGCATCAACTGCAAACTGCTGGTTCATAAGAAGTGCCACAAGCTTGTCAC AATTGAGTGTGGGCGGCATTCTTTGCCACCGGAACCCATGATGCCAATGGACCAGTCATCCATGCACCCA GACCACACACAGACAGTAATTCCATATAATCCTTCAAGTCATGAGAGTTTGGACCAAGTTGGTGAAGAAA AGGAGGCAATGAACACCAGGGAGAGTGGGAAGGCGTCATCAAGCTTAGGTCTCCAGGATTTCGATTTGCT TCGAGTTATAGGGAGAGGAAGTTACGCCAAAGTACTGCTGGTTCGATTAAAAAAGACAGATCGCATTTAT GCAATGAAGGTTGTGAAGAAAGAGCTCGTCAATGACGATGAGGATATTGATTGGGTACAGACAGAAAAGC ATGTGTTTGAGCAGGCGTCCAATCACCCTTTCCTTGTTGGTCTGCATTCCTGCTTCCAGACAGAAAGCAG GCTGTTTTTTGTCATAGAATATGTGAATGGAGGGGATCTCATGTTTCATATGCAGCGGCAAAGAAAACTT CCTGAAGAACATGCCAGGTTTTACTCAGCAGAAATCAGTCTAGCACTAAATTATCTTCATGAGCGAGGGA TAATTTATAGAGATTTGAAGTTGGACAATGTACTGCTGGACTCTGAAGGACACATCAAACTCACTGACTA CGGCATGTGTAAGGAAGGATTACGGCCCGGAGATACAACCAGCACCTTCTGTGGGACTCCCAATTACATT GCTCCTGAGATCTTAAGAGGAGAAGACTATGGCTTCAGCGTTGACTGGTGGGCTCTTGGAGTACTCATGT TTGAGATGATGGCGGGAAGGTCTCCATTTGATATCGTTGGGAGCTCTGACAATCCTGACCAAAACACAGA GGATTATCTGTTCCAAGTCATTTTGGAGAAGCAGATTCGCATACCGCGCTCCCTGTCTGTGAAAGCAGCA AGTGTGCTGAAGAGTTTCCTCAACAAGGACCCAAAGGAACGATTGGGTTGTCACCCTCAAACTGGATTTG CTGACATCCAAGGACACCCATTCTTCCGTAATGTGGATTGGGACATGATGGAGCAGAAGCAAGTGGTTCC GCCCTTTAAACCAAACATTTCTGGAGAATTTGGTTTGGATAACTTTGACTCCCAGTTTACCAACGAACCA GTCCAGCTCACTCCAGATGATGATGACATCGTGAGGAAGATTGATCAGTCTGAATTTGAAGGTTTCGAGT ATATCAACCCTCTCTTGATGTCTGCAGAAGAGTGTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205182 representing NM_032059
Red=Cloning site Green=Tags(s) MPTQRDSSTMSHTVACGGGGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCSEVRDMCSFDNEQPFTMKW IDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANG HTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPPEPMMPMDQSSMHP DHTQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIY AMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKL PEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYI APEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAA SVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEP VQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032059 |
| ORF Size | 1788 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032059.1, NP_114448.1 |
| RefSeq Size | 4501 bp |
| RefSeq ORF | 1791 bp |
| Locus ID | 84006 |
| UniProt ID | F1M7Y5 |
| MW | 68.3 kDa |
| Gene Summary | member of the atypical subgroup of PKCs that do not contain the calcium binding domain and possess only one cysteine-rich region; plays a critical role in controlling cell growth and cell survival; enhances Rab2-mediated vesicle budding [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205182 | Prkci (untagged ORF) - Rat protein kinase C, iota (Prkci), (10 ug) |
CNY 7,700.00 |
|
| RR205182L3 | Lenti ORF clone of Prkci (Myc-DDK-tagged ORF) - Rat protein kinase C, iota (Prkci), (10 ug) |
CNY 9,500.00 |
|
| RR205182L4 | Lenti ORF clone of Prkci (mGFP-tagged ORF) - Rat protein kinase C, iota (Prkci), (10 ug) |
CNY 10,360.00 |
