Col14a1 (NM_001130548) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208572 representing NM_001130548 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGATTTTGCAATGCAAGATGCGGGCCTGGCTGATTTTGGCTTTTTTGGCAGTTGCTTATTTCTGCA CCATCGTTCGGGGGCAAGTGGCTCCACCCACAAGGTTAAGATATAATGTAATATCCCATGACAGTATACA GATTTCATGGAAAGCACCAAGAGGGAAATTTGGTGGCTACAAACTGCTCGTGGCTCCTGCTTCAGGGGGA AAAACCAACCAGATGAATCTCCAGAACGGCGCAACAAAAGCCATTATTCAAGGCCTTCTGCCCGAGCAGA ATTACACCGTTCAACTTATTGCCTACTACAAAGACAAGGAAAGCAAGCCAGCTCAGGGCCAGTTCAGAAT TAAAGATTTAGAGAAAAGAAAGGATCCAACCAAGCCCAGAGTCAAGGCTGTGGACAAAGGAAACGGAAGT AAATCAACTTCACCAGAAGAAATAAAATTCTTCTGCGAGACTCCAGCCATCGCTGACATTGTCATCCTGG TGGATGGATCATGGAGTATTGGGAGATTCAACTTCAGGCTGGTGCGCAATTTCTTGGAAAACCTGGTCAC AGCGTTCAACGTGGGCTCAGAAAAGACAAGAATTGGCTTGGCACAGTACAGTGGGGACCCTAGAATAGAG TGGCACCTGAATGCCTTTAACACGAAAGATGAAGTGATTGACGCTGTCCGAAGCCTTCCATACAAAGGAG GAAATACCCTAACAGGTCTTGCTTTGAACTTTATTTTTGAGAATAGCTTTAAGCCAGAAGCAGGATCGAG GAGTGGCGTTTCCAAGATTGGCATTTTAATCACAGATGGAAAGTCCCAGGATGATATTATCCCACCTTCT AGAAACCTCCGTGAGGCTGGTGTAGAGTTGTTTGCAATAGGGGTTAAAAATGCCGACCTGAGCGAGCTGC AGGAGATAGCCTCTGAGCCAGACAGCACCCATGTGTACAATGTCGCTGAGTTCGACCTGATGCACACAGT GGTGGAGAGCCTGACCCGGACCGTATGCTCCAGAGTGGAAGAGCAGGACAAGGAGATTAAAGCCTCAGCC CTTGCCACCATTGGGCCACCTACAGAGCTGATTACGTCTGAAGTCACCGCCAGGAGCTTTATGGTTAACT GGACTCACTCCCCAGGAAAAGTGGAAAAATACAGAGTTGTGTATTATCCCACCAGAGGTGGAAAGCCAGA GGAGGTGGTGGCGGATGGAAGGGTGTCTTCCATAGTGTTGAAAAACTTGATGTCTTCAACCGAGTACCAG ATAGCAGTGTTTGCAGTATCTGCCCACACGGCTAGTGAAGGCCTGCGGGGAACAGAGACCACATTGGCCT TACCCATGGCTTCCGACCTCGAGCTGTATGATGTGACTGAGAACAGCATGCGAGTCAAGTGGGATGCAGT GCCTGGCGCCACAGGATATCTGATCCTCTATGCTCCTCTCACAGAAGGTCTGGCCGGAGATGAAAAAGAG ATGAAAATCGGTGAGACACACACAGACATTGAACTGAGTGGGCTATTCCCCAATACAGAATATACAGTCA CTGTGTATGCCATGTTTGGAGAAGAGGCCAGTGACCCTGCTACAGGACAAGAGACAACCCTACCCTTAAC CCCACCAAGAAATTTGAGGATCTCCAATGTCGGCTCTAACAGCGCTCGGTTAACCTGGGATCCCACATCG GGAAAGATCACTGGTTACAGAATTGTGTACACCAGTGCCGACGGGACAGAAATCAATGAGGTCGAAGTCG ATCCCATCACTACATTCCCTCTGAAAGGCTTGACCCCCCTCACAGACTACTCCATTGCCATTTTCTCCAT CTATGAGGAGGGGCAGTCCTTGCCTCTGGTTGGAGAGTTTACCACAGAGGAAGTTCCCGCCCAGCAATAC TTGGAAATTGATGAGGTGAAGACAGACAGTTTTCGAGTGACCTGGCATCCGCTCTCAGCTGAGGAGGGCC AGCACAAGCTGATGTGGATTCCGGTCTATGGCGGGAAGACTCAGGAGGTTGCCCTCAAAGAAGAGCAGGA CTCATATGTTGTTGAAGGCCTGGACCCTGGAACTGAGTATGAAGTCTCGCTATTAGCTGTGCTGGATGAC GGAAGTGAGAGTGAGGTGGTGACCGCTGTGGGGACCACACTTGATGATTTTTGGACTGAAGCACCCACAA CTGTAGAACCGACCAGCCCCGTGACTTCAGTTCTCCAGACAGGAATCAGAAACCTGGTTGTAGATGATGA AGCTGCTACTAGCCTGCGGGTCACATGGGACATTTCTGACAGCAATGTGGAGCACTTTAGGGTGACCTAC CTGACAGCCCAAGGGGACCCTAAGGAAGAAGTGGTTATGGTGCCTGGAGTGCAGAACAGTCTCCTTCTGA AAAATCTACTTCCAGACACAGAGTATAAAGTCACGGTGACACCCATCTACACTGTTGGAGAAGGCGTCAG CGTCTCTGCTCCTGGGAAGACTTTGCCAACTTCTGGTCCTCAAAACTTAAGGGTCTCAGAAGAATGGTAC AACCGGTTGCGCATTACATGGGACCCACCTTCTGCCCCCGTGAAGGGCTATAGGATTGTCTACAAACCTG TGAGTGTCCCTGGTCAAACTCTGGAAACTTTCGTGGGTGCTGACGTCAACACCATTGTCATGACAAATCT CCTCAGTGGAATGGACTACAATGTGAAGATCTTTGCTTCCCAGGCTGCAGGCTACAGCGACGCTCTGACT GGCCTTGTTCAAACACTGTTCTTGGGTGTGACAGATCTCCAAGCCAATCAGGTTGAAATGACAAGCTTGT GTGCTCGGTGGCAAATCCATCGCCATGCCACAGCCTACAGGATAGTTCTAGAATCCCTTCAGGACACACA AGCACAAGAATCCACCGTGGGAGGAGGAGTAAACAGGCATTGCTTCTATGGACTTCAGCCTGACTCAGAA TATAAAATCAGCGTTTATACAAAGCTCCAGGAGATAGAGGGACCGAGCGTGAGCATCATGCAGAAAACAC AATCACTTCCCACAGAACCACCAACTTTTCCTCCAACCATCCCACCAGCAAAGGAAGTATGTAAAGCAGC CAAGGCAGACCTAGTATTCATGGTGGATGGATCCTGGAGTATAGGAGATGACAATTTCAATAAGATCATC AACTTTCTGTATAGCACAGTGGGAGCCCTGGACAAGATTGGTGCAGATGGAACTCAGGTGGCAATGGTTC AGTTCACTGATGACCCCCGAACAGAATTCAAACTCGATGCTTACAAAACCAAAGAGACACTTCTTGATGC AATTAGACACATTTCCTACAAAGGGGGAAATACTAAAACAGGAAAAGCTATCAAGCACGTTCGAGATACC TTATTTACTGCAGACTCAGGTACAAGGAGAGGCATCCCAAAGGTCATTGTGGTGATAACTGATGGACGGT CACAAGATGATGTCAACAAAATCTCCAGGGAGATGCAGGCAGATGGTTACAATATCTTTGCCATCGGTGT GGCTGATGCTGATTACTCAGAGTTGGTTCGGATCGGCAGCAAGCCCAGCTCACGTCACGTCTTCTTTGTG GATGACTTTGATGCCTTTAAGAAAATTGAAGATGAATTAATTACTTTTGTCTGTGAAACTGCATCAGCAA CCTGCCCAATGGTGCACAAGGATGGAGTTGATATTGCAGGATTTAAGATGATGGAAATGTTTGGTTTGGT TGAAAAGGATTTTTCTGCAGTGGAAGGAGTGTCTATGGAACCTGGTACCTTCAATTTGTTCCCATGCTAT CAAATCCATAAGGATGCCCTGGTTTCCCAGCCAACAAAGTATCTGCACCCAGAAGGATTGCCTGCTGACT ATACAATGACTTTTCTGTTCCGGATTCTTCCTGACACGCCACAGGAGCCATTTGCTCTTTGGGAGATTTT AAATAAAAAGTCTGAGCCGCTAGTTGGGATAATTTTGGACAATGGTGGAAAGACTCTAACTTATTTCAGC TATGACTATACTGGAGATTTTCAAACTGTCACTTTTGAAGGACCTGACATCAGGAAAATGTTCTATGGAA GCTTTCACAAGCTCCATGTTGTTGTCAGCAAGACTTTGGCCAAAGTGGTTGTCGACTGTAAAGAAGTTGG CCAGAAGGCAATAAATGCGTCAGCGAATATCACTTCGGATGGTGTGGAAGTCCTAGGGAGAATGGTCAGA TCGAGAGGGCCAAATGGAAACTCTGCACCATTCCAGTTACAAATGTTTGATATCGTTTGCTCTACATCAT GGGCCAGTAAAGACAGATGCTGTGAACTTCCGGGCCTGCGGGATGAAGAGTCCTGCCCAGACCTTCCACG ATCCTGCTCTTGCTCAGAAACCAATGAAGTGGCTCTAGGACCAGCTGGCCCCCCAGGTGGTCCAGGACTC CGAGGACCAAAAGGCCAACAAGGTGAACCGGGTCCGAAGGGACCAGAGGGACCTCGGGGAGAAACAGGTC CTGCAGGACCCCAGGGCCCCCCTGGCCCCCAAGGACCCAGTGGTCTGTCCATTCAAGGCATGCCTGGAAT GCCTGGTGATAAAGGAGATAAAGGAGATGCCGGCCTTCCTGGTCCACAGGGTGTCCCTGGAGGTGTTGGT TCACCAGGACGCGATGGCTCACCAGGCCAGAGGGGATTTCCCGGAAAGGATGGTTCCTCTGGGCCTCCAG GACCACCTGGACCAATTGGCATTCCTGGAGCTCCTGGTGTACCAGGGATTGCGGGCAGCATGGGACCACA AGGAGCCTTGGGGCCACCTGGTGTCCCTGGAGCAAAGGGAGAGCGAGGTGAACGAGGAGACCTGCAGTCT CAAGCCATGGTGAGGGCAGTGGCTCGTCAAGTATGTGAGCAGCTCATTCAGAGTCACATGGCCAGGTACA CAGCCATCCTCAACCAAATCCCCAGCCAGTCCTCATCTATCCGGACCATCCAGGGCCCTCCTGGAGAGCC CGGAAGACCAGGTTCACCTGGAACCCCTGGAGAACAAGGACCCCCAGGTGCCCCAGGCTTCCCAGGAAAT GCAGGAGTGCCAGGAACCCCAGGAGAACGAGGTCTAACTGGTGTCAAAGGAGATAAAGGAAACCCAGGCA TTGGAACTCAAGGCCCAAGAGGTCCACCTGGGCCAGCAGGACCTTCAGGGGAAAGTCGTCCTGGCAGTCC TGGGCCACCTGGCTCTCCTGGACCAAGGGGTCCCCCGGGCCACCTGGGAGTACCTGGGCCACAGGGTCCT TCTGGCCAGCCTGGATATTGTGACCCCTCCTCGTGTTCTGCTTACGGCGTTGGAGTGTCCCATCCAGATC AACCCGAGTTCACACCGGTCCAGGACGAGCAGGAGGCCTTGGATCTGTGGAGCGCAGGCATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001130548
Insert Size	5385 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001130548.1, NP_001124020.1
RefSeq Size	6437 bp
RefSeq ORF	5385 bp
Locus ID	314981

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