Col14a1 (NM_001130548) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208572 representing NM_001130548 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGATTTTGCAATGCAAGATGCGGGCCTGGCTGATTTTGGCTTTTTTGGCAGTTGCTTATTTCTGCA CCATCGTTCGGGGGCAAGTGGCTCCACCCACAAGGTTAAGATATAATGTAATATCCCATGACAGTATACA GATTTCATGGAAAGCACCAAGAGGGAAATTTGGTGGCTACAAACTGCTCGTGGCTCCTGCTTCAGGGGGA AAAACCAACCAGATGAATCTCCAGAACGGCGCAACAAAAGCCATTATTCAAGGCCTTCTGCCCGAGCAGA ATTACACCGTTCAACTTATTGCCTACTACAAAGACAAGGAAAGCAAGCCAGCTCAGGGCCAGTTCAGAAT TAAAGATTTAGAGAAAAGAAAGGATCCAACCAAGCCCAGAGTCAAGGCTGTGGACAAAGGAAACGGAAGT AAATCAACTTCACCAGAAGAAATAAAATTCTTCTGCGAGACTCCAGCCATCGCTGACATTGTCATCCTGG TGGATGGATCATGGAGTATTGGGAGATTCAACTTCAGGCTGGTGCGCAATTTCTTGGAAAACCTGGTCAC AGCGTTCAACGTGGGCTCAGAAAAGACAAGAATTGGCTTGGCACAGTACAGTGGGGACCCTAGAATAGAG TGGCACCTGAATGCCTTTAACACGAAAGATGAAGTGATTGACGCTGTCCGAAGCCTTCCATACAAAGGAG GAAATACCCTAACAGGTCTTGCTTTGAACTTTATTTTTGAGAATAGCTTTAAGCCAGAAGCAGGATCGAG GAGTGGCGTTTCCAAGATTGGCATTTTAATCACAGATGGAAAGTCCCAGGATGATATTATCCCACCTTCT AGAAACCTCCGTGAGGCTGGTGTAGAGTTGTTTGCAATAGGGGTTAAAAATGCCGACCTGAGCGAGCTGC AGGAGATAGCCTCTGAGCCAGACAGCACCCATGTGTACAATGTCGCTGAGTTCGACCTGATGCACACAGT GGTGGAGAGCCTGACCCGGACCGTATGCTCCAGAGTGGAAGAGCAGGACAAGGAGATTAAAGCCTCAGCC CTTGCCACCATTGGGCCACCTACAGAGCTGATTACGTCTGAAGTCACCGCCAGGAGCTTTATGGTTAACT GGACTCACTCCCCAGGAAAAGTGGAAAAATACAGAGTTGTGTATTATCCCACCAGAGGTGGAAAGCCAGA GGAGGTGGTGGCGGATGGAAGGGTGTCTTCCATAGTGTTGAAAAACTTGATGTCTTCAACCGAGTACCAG ATAGCAGTGTTTGCAGTATCTGCCCACACGGCTAGTGAAGGCCTGCGGGGAACAGAGACCACATTGGCCT TACCCATGGCTTCCGACCTCGAGCTGTATGATGTGACTGAGAACAGCATGCGAGTCAAGTGGGATGCAGT GCCTGGCGCCACAGGATATCTGATCCTCTATGCTCCTCTCACAGAAGGTCTGGCCGGAGATGAAAAAGAG ATGAAAATCGGTGAGACACACACAGACATTGAACTGAGTGGGCTATTCCCCAATACAGAATATACAGTCA CTGTGTATGCCATGTTTGGAGAAGAGGCCAGTGACCCTGCTACAGGACAAGAGACAACCCTACCCTTAAC CCCACCAAGAAATTTGAGGATCTCCAATGTCGGCTCTAACAGCGCTCGGTTAACCTGGGATCCCACATCG GGAAAGATCACTGGTTACAGAATTGTGTACACCAGTGCCGACGGGACAGAAATCAATGAGGTCGAAGTCG ATCCCATCACTACATTCCCTCTGAAAGGCTTGACCCCCCTCACAGACTACTCCATTGCCATTTTCTCCAT CTATGAGGAGGGGCAGTCCTTGCCTCTGGTTGGAGAGTTTACCACAGAGGAAGTTCCCGCCCAGCAATAC TTGGAAATTGATGAGGTGAAGACAGACAGTTTTCGAGTGACCTGGCATCCGCTCTCAGCTGAGGAGGGCC AGCACAAGCTGATGTGGATTCCGGTCTATGGCGGGAAGACTCAGGAGGTTGCCCTCAAAGAAGAGCAGGA CTCATATGTTGTTGAAGGCCTGGACCCTGGAACTGAGTATGAAGTCTCGCTATTAGCTGTGCTGGATGAC GGAAGTGAGAGTGAGGTGGTGACCGCTGTGGGGACCACACTTGATGATTTTTGGACTGAAGCACCCACAA CTGTAGAACCGACCAGCCCCGTGACTTCAGTTCTCCAGACAGGAATCAGAAACCTGGTTGTAGATGATGA AGCTGCTACTAGCCTGCGGGTCACATGGGACATTTCTGACAGCAATGTGGAGCACTTTAGGGTGACCTAC CTGACAGCCCAAGGGGACCCTAAGGAAGAAGTGGTTATGGTGCCTGGAGTGCAGAACAGTCTCCTTCTGA AAAATCTACTTCCAGACACAGAGTATAAAGTCACGGTGACACCCATCTACACTGTTGGAGAAGGCGTCAG CGTCTCTGCTCCTGGGAAGACTTTGCCAACTTCTGGTCCTCAAAACTTAAGGGTCTCAGAAGAATGGTAC AACCGGTTGCGCATTACATGGGACCCACCTTCTGCCCCCGTGAAGGGCTATAGGATTGTCTACAAACCTG TGAGTGTCCCTGGTCAAACTCTGGAAACTTTCGTGGGTGCTGACGTCAACACCATTGTCATGACAAATCT CCTCAGTGGAATGGACTACAATGTGAAGATCTTTGCTTCCCAGGCTGCAGGCTACAGCGACGCTCTGACT GGCCTTGTTCAAACACTGTTCTTGGGTGTGACAGATCTCCAAGCCAATCAGGTTGAAATGACAAGCTTGT GTGCTCGGTGGCAAATCCATCGCCATGCCACAGCCTACAGGATAGTTCTAGAATCCCTTCAGGACACACA AGCACAAGAATCCACCGTGGGAGGAGGAGTAAACAGGCATTGCTTCTATGGACTTCAGCCTGACTCAGAA TATAAAATCAGCGTTTATACAAAGCTCCAGGAGATAGAGGGACCGAGCGTGAGCATCATGCAGAAAACAC AATCACTTCCCACAGAACCACCAACTTTTCCTCCAACCATCCCACCAGCAAAGGAAGTATGTAAAGCAGC CAAGGCAGACCTAGTATTCATGGTGGATGGATCCTGGAGTATAGGAGATGACAATTTCAATAAGATCATC AACTTTCTGTATAGCACAGTGGGAGCCCTGGACAAGATTGGTGCAGATGGAACTCAGGTGGCAATGGTTC AGTTCACTGATGACCCCCGAACAGAATTCAAACTCGATGCTTACAAAACCAAAGAGACACTTCTTGATGC AATTAGACACATTTCCTACAAAGGGGGAAATACTAAAACAGGAAAAGCTATCAAGCACGTTCGAGATACC TTATTTACTGCAGACTCAGGTACAAGGAGAGGCATCCCAAAGGTCATTGTGGTGATAACTGATGGACGGT CACAAGATGATGTCAACAAAATCTCCAGGGAGATGCAGGCAGATGGTTACAATATCTTTGCCATCGGTGT GGCTGATGCTGATTACTCAGAGTTGGTTCGGATCGGCAGCAAGCCCAGCTCACGTCACGTCTTCTTTGTG GATGACTTTGATGCCTTTAAGAAAATTGAAGATGAATTAATTACTTTTGTCTGTGAAACTGCATCAGCAA CCTGCCCAATGGTGCACAAGGATGGAGTTGATATTGCAGGATTTAAGATGATGGAAATGTTTGGTTTGGT TGAAAAGGATTTTTCTGCAGTGGAAGGAGTGTCTATGGAACCTGGTACCTTCAATTTGTTCCCATGCTAT CAAATCCATAAGGATGCCCTGGTTTCCCAGCCAACAAAGTATCTGCACCCAGAAGGATTGCCTGCTGACT ATACAATGACTTTTCTGTTCCGGATTCTTCCTGACACGCCACAGGAGCCATTTGCTCTTTGGGAGATTTT AAATAAAAAGTCTGAGCCGCTAGTTGGGATAATTTTGGACAATGGTGGAAAGACTCTAACTTATTTCAGC TATGACTATACTGGAGATTTTCAAACTGTCACTTTTGAAGGACCTGACATCAGGAAAATGTTCTATGGAA GCTTTCACAAGCTCCATGTTGTTGTCAGCAAGACTTTGGCCAAAGTGGTTGTCGACTGTAAAGAAGTTGG CCAGAAGGCAATAAATGCGTCAGCGAATATCACTTCGGATGGTGTGGAAGTCCTAGGGAGAATGGTCAGA TCGAGAGGGCCAAATGGAAACTCTGCACCATTCCAGTTACAAATGTTTGATATCGTTTGCTCTACATCAT GGGCCAGTAAAGACAGATGCTGTGAACTTCCGGGCCTGCGGGATGAAGAGTCCTGCCCAGACCTTCCACG ATCCTGCTCTTGCTCAGAAACCAATGAAGTGGCTCTAGGACCAGCTGGCCCCCCAGGTGGTCCAGGACTC CGAGGACCAAAAGGCCAACAAGGTGAACCGGGTCCGAAGGGACCAGAGGGACCTCGGGGAGAAACAGGTC CTGCAGGACCCCAGGGCCCCCCTGGCCCCCAAGGACCCAGTGGTCTGTCCATTCAAGGCATGCCTGGAAT GCCTGGTGATAAAGGAGATAAAGGAGATGCCGGCCTTCCTGGTCCACAGGGTGTCCCTGGAGGTGTTGGT TCACCAGGACGCGATGGCTCACCAGGCCAGAGGGGATTTCCCGGAAAGGATGGTTCCTCTGGGCCTCCAG GACCACCTGGACCAATTGGCATTCCTGGAGCTCCTGGTGTACCAGGGATTGCGGGCAGCATGGGACCACA AGGAGCCTTGGGGCCACCTGGTGTCCCTGGAGCAAAGGGAGAGCGAGGTGAACGAGGAGACCTGCAGTCT CAAGCCATGGTGAGGGCAGTGGCTCGTCAAGTATGTGAGCAGCTCATTCAGAGTCACATGGCCAGGTACA CAGCCATCCTCAACCAAATCCCCAGCCAGTCCTCATCTATCCGGACCATCCAGGGCCCTCCTGGAGAGCC CGGAAGACCAGGTTCACCTGGAACCCCTGGAGAACAAGGACCCCCAGGTGCCCCAGGCTTCCCAGGAAAT GCAGGAGTGCCAGGAACCCCAGGAGAACGAGGTCTAACTGGTGTCAAAGGAGATAAAGGAAACCCAGGCA TTGGAACTCAAGGCCCAAGAGGTCCACCTGGGCCAGCAGGACCTTCAGGGGAAAGTCGTCCTGGCAGTCC TGGGCCACCTGGCTCTCCTGGACCAAGGGGTCCCCCGGGCCACCTGGGAGTACCTGGGCCACAGGGTCCT TCTGGCCAGCCTGGATATTGTGACCCCTCCTCGTGTTCTGCTTACGGCGTTGGAGTGTCCCATCCAGATC AACCCGAGTTCACACCGGTCCAGGACGAGCAGGAGGCCTTGGATCTGTGGAGCGCAGGCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208572 representing NM_001130548 Red=Cloning site Green=Tags(s) MTILQCKMRAWLILAFLAVAYFCTIVRGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVAPASGG KTNQMNLQNGATKAIIQGLLPEQNYTVQLIAYYKDKESKPAQGQFRIKDLEKRKDPTKPRVKAVDKGNGS KSTSPEEIKFFCETPAIADIVILVDGSWSIGRFNFRLVRNFLENLVTAFNVGSEKTRIGLAQYSGDPRIE WHLNAFNTKDEVIDAVRSLPYKGGNTLTGLALNFIFENSFKPEAGSRSGVSKIGILITDGKSQDDIIPPS RNLREAGVELFAIGVKNADLSELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTVCSRVEEQDKEIKASA LATIGPPTELITSEVTARSFMVNWTHSPGKVEKYRVVYYPTRGGKPEEVVADGRVSSIVLKNLMSSTEYQ IAVFAVSAHTASEGLRGTETTLALPMASDLELYDVTENSMRVKWDAVPGATGYLILYAPLTEGLAGDEKE MKIGETHTDIELSGLFPNTEYTVTVYAMFGEEASDPATGQETTLPLTPPRNLRISNVGSNSARLTWDPTS GKITGYRIVYTSADGTEINEVEVDPITTFPLKGLTPLTDYSIAIFSIYEEGQSLPLVGEFTTEEVPAQQY LEIDEVKTDSFRVTWHPLSAEEGQHKLMWIPVYGGKTQEVALKEEQDSYVVEGLDPGTEYEVSLLAVLDD GSESEVVTAVGTTLDDFWTEAPTTVEPTSPVTSVLQTGIRNLVVDDEAATSLRVTWDISDSNVEHFRVTY LTAQGDPKEEVVMVPGVQNSLLLKNLLPDTEYKVTVTPIYTVGEGVSVSAPGKTLPTSGPQNLRVSEEWY NRLRITWDPPSAPVKGYRIVYKPVSVPGQTLETFVGADVNTIVMTNLLSGMDYNVKIFASQAAGYSDALT GLVQTLFLGVTDLQANQVEMTSLCARWQIHRHATAYRIVLESLQDTQAQESTVGGGVNRHCFYGLQPDSE YKISVYTKLQEIEGPSVSIMQKTQSLPTEPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDDNFNKII NFLYSTVGALDKIGADGTQVAMVQFTDDPRTEFKLDAYKTKETLLDAIRHISYKGGNTKTGKAIKHVRDT LFTADSGTRRGIPKVIVVITDGRSQDDVNKISREMQADGYNIFAIGVADADYSELVRIGSKPSSRHVFFV DDFDAFKKIEDELITFVCETASATCPMVHKDGVDIAGFKMMEMFGLVEKDFSAVEGVSMEPGTFNLFPCY QIHKDALVSQPTKYLHPEGLPADYTMTFLFRILPDTPQEPFALWEILNKKSEPLVGIILDNGGKTLTYFS YDYTGDFQTVTFEGPDIRKMFYGSFHKLHVVVSKTLAKVVVDCKEVGQKAINASANITSDGVEVLGRMVR SRGPNGNSAPFQLQMFDIVCSTSWASKDRCCELPGLRDEESCPDLPRSCSCSETNEVALGPAGPPGGPGL RGPKGQQGEPGPKGPEGPRGETGPAGPQGPPGPQGPSGLSIQGMPGMPGDKGDKGDAGLPGPQGVPGGVG SPGRDGSPGQRGFPGKDGSSGPPGPPGPIGIPGAPGVPGIAGSMGPQGALGPPGVPGAKGERGERGDLQS QAMVRAVARQVCEQLIQSHMARYTAILNQIPSQSSSIRTIQGPPGEPGRPGSPGTPGEQGPPGAPGFPGN AGVPGTPGERGLTGVKGDKGNPGIGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGP SGQPGYCDPSSCSAYGVGVSHPDQPEFTPVQDEQEALDLWSAGI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001130548
ORF Size	5382 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001130548.1, NP_001124020.1
RefSeq Size	6437 bp
RefSeq ORF	5385 bp
Locus ID	314981
MW	192.6 kDa

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