Alox12b (NM_001039377) Rat Untagged Clone

CAT#: RN211780

Alox12b (untagged ORF) - Rat arachidonate 12-lipoxygenase, 12R type (Alox12b), (10 ug)



  "NM_001039377" in other vectors (3)

CNY 9,030.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN211780 representing NM_001039377
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCTACAAAGTCAAGGTGGCCACGGGCACTGACTTCTTTTCGGGGACGCTGGACTCAATTTCCC
TGACCATCGTGGGCACCCAAGGAGAGAGCCATAAGCAGCGGTTGAACCACTTTGGGAGAGACTTTGCCAC
AGGGGCCGTGGATGACTACACAGTGCAGTGCCAGCAGGACCTGGGGGAGCTCATCATCATCCGTCTGCAC
AAGGAGCCTCATTCCTTCTTACCCAAGGACCCCTGGTACTGCAACTATGTGCAGATCTGCGCTCCCAACT
GTCGTGTCTACCACTTCCCAGCTTACCAGTGGATGGATGGCTATGAGACCCTGTCACTCCGGGAGGCCAC
AGGAAAGACAACAGCAGATGACACACTTCCCATCCTTTTGGAACACAGACAAGAAGAGATCAGAGCCAAG
AAAGACTTCTACCACTGGAGGGTCTTTGTTCCCGGCCTTCCCAACTATGTGGACATCCCCAGTTACCATC
CTCCTCCCAGACGGTGCCGCAACCCCAACCGGCCTGAGTGGAATGGTTATATTCCAGGATTCCCAATTCT
CATCAACATTAAGGCCACCAGGTTCTTAAACTTAAATCTTCGTTTCTCCTTCGTCAAGACAGCCTCCTTC
TTCTACCGCCTAGGGCCCATGGCACTGGCCTTCAAGCTTCGTGGCCTGGTGGATCGCAAACGCTCTTGGA
AGAGACTAAAGGACATTAAGAACATTTTCCCTGCTACCAAGACTGTCGTCTCTGAGTATGTGGCCGAGCA
CTGGACTGAAGATAGCTTCTTTGGGTACCAGTACCTCAACGGCATCAACCCAGGCCATATCCGAAGATGC
ATGCAGATCCCAGACAAGTTCCCAGTTACAGATGAAATGGTGGCCCCATTCCTGGGTGAAGGAACATGCC
TGCAGGCAGAGCTGGAGAAGGGGAATATCTACCTGGCAGACTACCGCATCTTGGATGGCATCCCCACGGT
GGAGCTCAATGGTCAGAAGCAGCATCACTGTGCCCCGATCTGCTTGCTGCACTTTGGTCCCGATGGTAAC
ATGATGCCTATTGCCATTCAGCTCAGCCAGACTCCTGGGCCTGATTGTCCCATCTTCCTGCCTAATGATT
CTGAGTGGGACTGGCTGTTGGCTAAAACATGGGTACGCTACGCTGAGTTCTACAGCCACGAGGCCGTTGC
CCACTTACTGGAGAGTCACCTCATCGGGGAAGCTTTCTGCTTGGCCCTCCTGAGGAACCTGCCCATGTGC
CACCCCCTGTATAAGCTCCTCATTCCTCACACTCGCTACAACGTCCAGATCAACAGCATTGGGCGGGCCC
TCCTTCTCAACAAAGGGGGTCTCTCTGCCAGGGCCATGTCCCTGGGCCTAGAGGGCTTCGCACAGGTGAT
GGTTCGGGGTCTGTCTGAGCTCACCTACAAAAGCCTCTGCATCCCCAATGACTTTGTGGAGCGCGGGGTT
CAGGATCTGCCTGGGTATTATTTCCGTGACGACAGCTTGGCGGTGTGGTATGCCATGGAGAGGTACGTGA
CAGAGATCATCACTTATTATTACCCGAATGACGCAGCTGTGGAGGGCGACCCGGAATTGCAGTGCTGGGT
GCAGGAGATATTCAAGGAGTGTCTCCTGGAGCGTGAGAGCTCAGGTTTTCCCACCTGCTTGCGAACTGTA
CCTGAGCTGATCGAATACGTCACGATGGTCATGTACACCTGCTCAGCGAGGCATGCAGCTGTCAACACAG
GCCAGTTGGAATACACTTCTTGGATGCCCAACTTCCCGTCATCCATGAGGAACCCGCCGATGCAGTCCAA
GGGGCTGACTACTTTGCAGACATTCATGGACACTTTGCCAGACGTGAAGACCACATGCATTGTGCTCTTG
GTGCTCTGGACTCTCTGCCGGGAGCCTGATGACAGGCGGCCACTTGGCCACTTCCCGGACATCCATTTTG
TGGAGGAGGCCCCTCGGAGGAGTATGGAGGCTTTTCGTCAGAACCTCAACCAGATCTCTCACAACATCCG
CCAGCGCAATAAGTGCCTCAACCTCCCGTATTACTACCTGGACCCGGTGCTGATTGAGAACAGTATTTCC
ATCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001039377
Insert Size 2106 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001039377.1, NP_001034466.1
RefSeq Size 2268 bp
RefSeq ORF 2106 bp
Locus ID 287425
UniProt ID Q2KMM4
Gene Summary Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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