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Alox12b (NM_001039377) Rat Tagged ORF Clone
CAT＃: RR211780
- TrueORF®
Alox12b (Myc-DDK-tagged ORF) - Rat arachidonate 12-lipoxygenase, 12R type (Alox12b), (10 ug)
"NM_001039377" in other vectors (3)
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CNY 9,030.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211780 representing NM_001039377
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCTACAAAGTCAAGGTGGCCACGGGCACTGACTTCTTTTCGGGGACGCTGGACTCAATTTCCC TGACCATCGTGGGCACCCAAGGAGAGAGCCATAAGCAGCGGTTGAACCACTTTGGGAGAGACTTTGCCAC AGGGGCCGTGGATGACTACACAGTGCAGTGCCAGCAGGACCTGGGGGAGCTCATCATCATCCGTCTGCAC AAGGAGCCTCATTCCTTCTTACCCAAGGACCCCTGGTACTGCAACTATGTGCAGATCTGCGCTCCCAACT GTCGTGTCTACCACTTCCCAGCTTACCAGTGGATGGATGGCTATGAGACCCTGTCACTCCGGGAGGCCAC AGGAAAGACAACAGCAGATGACACACTTCCCATCCTTTTGGAACACAGACAAGAAGAGATCAGAGCCAAG AAAGACTTCTACCACTGGAGGGTCTTTGTTCCCGGCCTTCCCAACTATGTGGACATCCCCAGTTACCATC CTCCTCCCAGACGGTGCCGCAACCCCAACCGGCCTGAGTGGAATGGTTATATTCCAGGATTCCCAATTCT CATCAACATTAAGGCCACCAGGTTCTTAAACTTAAATCTTCGTTTCTCCTTCGTCAAGACAGCCTCCTTC TTCTACCGCCTAGGGCCCATGGCACTGGCCTTCAAGCTTCGTGGCCTGGTGGATCGCAAACGCTCTTGGA AGAGACTAAAGGACATTAAGAACATTTTCCCTGCTACCAAGACTGTCGTCTCTGAGTATGTGGCCGAGCA CTGGACTGAAGATAGCTTCTTTGGGTACCAGTACCTCAACGGCATCAACCCAGGCCATATCCGAAGATGC ATGCAGATCCCAGACAAGTTCCCAGTTACAGATGAAATGGTGGCCCCATTCCTGGGTGAAGGAACATGCC TGCAGGCAGAGCTGGAGAAGGGGAATATCTACCTGGCAGACTACCGCATCTTGGATGGCATCCCCACGGT GGAGCTCAATGGTCAGAAGCAGCATCACTGTGCCCCGATCTGCTTGCTGCACTTTGGTCCCGATGGTAAC ATGATGCCTATTGCCATTCAGCTCAGCCAGACTCCTGGGCCTGATTGTCCCATCTTCCTGCCTAATGATT CTGAGTGGGACTGGCTGTTGGCTAAAACATGGGTACGCTACGCTGAGTTCTACAGCCACGAGGCCGTTGC CCACTTACTGGAGAGTCACCTCATCGGGGAAGCTTTCTGCTTGGCCCTCCTGAGGAACCTGCCCATGTGC CACCCCCTGTATAAGCTCCTCATTCCTCACACTCGCTACAACGTCCAGATCAACAGCATTGGGCGGGCCC TCCTTCTCAACAAAGGGGGTCTCTCTGCCAGGGCCATGTCCCTGGGCCTAGAGGGCTTCGCACAGGTGAT GGTTCGGGGTCTGTCTGAGCTCACCTACAAAAGCCTCTGCATCCCCAATGACTTTGTGGAGCGCGGGGTT CAGGATCTGCCTGGGTATTATTTCCGTGACGACAGCTTGGCGGTGTGGTATGCCATGGAGAGGTACGTGA CAGAGATCATCACTTATTATTACCCGAATGACGCAGCTGTGGAGGGCGACCCGGAATTGCAGTGCTGGGT GCAGGAGATATTCAAGGAGTGTCTCCTGGAGCGTGAGAGCTCAGGTTTTCCCACCTGCTTGCGAACTGTA CCTGAGCTGATCGAATACGTCACGATGGTCATGTACACCTGCTCAGCGAGGCATGCAGCTGTCAACACAG GCCAGTTGGAATACACTTCTTGGATGCCCAACTTCCCGTCATCCATGAGGAACCCGCCGATGCAGTCCAA GGGGCTGACTACTTTGCAGACATTCATGGACACTTTGCCAGACGTGAAGACCACATGCATTGTGCTCTTG GTGCTCTGGACTCTCTGCCGGGAGCCTGATGACAGGCGGCCACTTGGCCACTTCCCGGACATCCATTTTG TGGAGGAGGCCCCTCGGAGGAGTATGGAGGCTTTTCGTCAGAACCTCAACCAGATCTCTCACAACATCCG CCAGCGCAATAAGTGCCTCAACCTCCCGTATTACTACCTGGACCCGGTGCTGATTGAGAACAGTATTTCC ATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211780 representing NM_001039377
Red=Cloning site Green=Tags(s) MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRDFATGAVDDYTVQCQQDLGELIIIRLH KEPHSFLPKDPWYCNYVQICAPNCRVYHFPAYQWMDGYETLSLREATGKTTADDTLPILLEHRQEEIRAK KDFYHWRVFVPGLPNYVDIPSYHPPPRRCRNPNRPEWNGYIPGFPILINIKATRFLNLNLRFSFVKTASF FYRLGPMALAFKLRGLVDRKRSWKRLKDIKNIFPATKTVVSEYVAEHWTEDSFFGYQYLNGINPGHIRRC MQIPDKFPVTDEMVAPFLGEGTCLQAELEKGNIYLADYRILDGIPTVELNGQKQHHCAPICLLHFGPDGN MMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMC HPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGV QDLPGYYFRDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLERESSGFPTCLRTV PELIEYVTMVMYTCSARHAAVNTGQLEYTSWMPNFPSSMRNPPMQSKGLTTLQTFMDTLPDVKTTCIVLL VLWTLCREPDDRRPLGHFPDIHFVEEAPRRSMEAFRQNLNQISHNIRQRNKCLNLPYYYLDPVLIENSIS I TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039377 |
| ORF Size | 2103 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039377.1, NP_001034466.1 |
| RefSeq Size | 2268 bp |
| RefSeq ORF | 2106 bp |
| Locus ID | 287425 |
| UniProt ID | Q2KMM4 |
| MW | 80.7 kDa |
| Gene Summary | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211780 | Alox12b (untagged ORF) - Rat arachidonate 12-lipoxygenase, 12R type (Alox12b), (10 ug) |
CNY 9,030.00 |
|
| RR211780L3 | Lenti ORF clone of Alox12b (Myc-DDK-tagged ORF) - Rat arachidonate 12-lipoxygenase, 12R type (Alox12b), (10 ug) |
CNY 10,830.00 |
|
| RR211780L4 | Lenti ORF clone of Alox12b (mGFP-tagged ORF) - Rat arachidonate 12-lipoxygenase, 12R type (Alox12b), (10 ug) |
CNY 11,880.00 |
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