Gak (NM_031030) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215048 representing NM_031030 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTGCTGCAGTCTGCACTGGACTTCCTGGCGGGCCCTGGTTCTCTTGGCGGAGCTGCCGGCCGTG ACCAGAGTGACTTCGTGGGGCAGACTGTGGAGCTGGGCGAGCTGCGTCTGCGGGTGCGGCGGGTCCTGGC CGAGGGAGGGTTTGCATTTGTTTATGAAGCTCAAGATCTGGGAAGTGGCAGAGAGTATGCATTAAAGAGA TTACTATCCAATGAAGAGGAAAAGAACAGAGCCATCATTCAGGAAGTATGTTTCTTGAAAAAACTTTCTG GCCACCCCAATATTGTCCAGTTCTGCTCTGCAGCATCCATAGGAAAAGAGGAATCGGACACTGGGCAGGC TGAGTTCCTCCTGCTTACGGAGCTCTGTAAAGGACAGCTGGTGGAGTTTCTCAGGAGAGTTGAATGTAAA GGCCCTCTATCCTGCGACAGCATTCTGAAGATCTTCTACCAGACATGCAGAGCAGTGCAGCACATGCACA GGCAGAAACCACCCATCATCCACAGGGATCTCAAGGTTGAAAACTTACTGCTTAGTAACCAGGGGACCAT TAAGCTGTGTGACTTTGGCAGTGCCACAACCATCTCCCATTATCCTGACTACAGCTGGAGCGCCCAGAAG CGAGCAATGGTGGAGGAAGAGATCACGAGGAACACCACACCCATGTACAGAACGCCAGAAATTGTAGACC TGTATTCTAACTTCCCTATTGGCGAAAAGCAGGATATCTGGGCACTGGGCTGTATCTTATACCTGCTGTG TTTCCGGCAGCATCCTTTTGAAGATGGAGCAAAACTTCGGATAGTCAATGGGAAGTATTCCATTCCTGTG AATGACACTCGTTACACAGTCTTCCATGACCTTATTCGTGGCATGCTAAAGGTCAATCCAGAAGAGAGGC TATCCATTGCTGAAGTTGTCCGACAACTGCAGGAAATTGCAGCAGCCCGGAATGTGAACCCCAAAGCCCC CATCACAGAACTTCTGGAGCAGAATGGTGGCTATGGGAACTCAGGGCCTTCCCGAGCACAACCACCTTCT GGGGGCCCTGTGAACAGCAGTGGAGTTTTGGCTCTGGCAGAGTATGACCAGCCCTATGGTGGGTTTCTCG ATATCCTACGGGGTGGGACAGAACGGCTCTTCACCAACCTCAAGGATACTTCCTCCAAGGTCATCCAGTC TGTGGCTAACTATGCAAAGGGCGATCTTGACATATCTTACATCACATCCAGGATTGCAGTGATGTCGTTC CCAGCAGAAGGTGTGGAGTCAGCAATCAAAAACAATATAGAGGATGTACGATTGTTTCTGGATGCCAAGC ATCCAGGACATTATGCTGTCTACAACCTTTCTCCAAGAATATACCGGGCTTCCAAGTTCCACAATCGGGT CACTGAGTGTGGCTGGGCAGTCAGGCGGGCACCACATCTCCACAGTTTGTATACTCTATGCAGGAGCATG CATGCCTGGCTCCGGGAAGACCACAGGAACGTCTGTGTTGTACATTGCATGGATGGGAGAGCTGCGTCTG CTGTGGCAGTCTGTGCATTCCTGTGCTTCTGCCGTCTCTTCAGCACTGCAGAGGCTGCTGTGTACATGTT CAGCATGAAGCGCTGCCCACCAGGCATTTGGCCATCCCACAAAAGGTACATTGAATACGTGTGTGACATG GTGGCAGAGGAACCCATCACGCCCCACAGCAAGCCAATGCTGGTGAAATCTGTTGTCATGACCCCTGTGC CACTGTTCAGCAAGCAGAGGAATGGCTGCCGACCATTCTGTGAGGTCTATGTTGGGGAGGAGCGTGTAAC CACCACATCCCAGGAGTATGACAGGATGAAGGAATTTAAAATTGAGGATGGCAAGGCTGTCATCCCCCTG GGCATAACAGTTCAAGGCGATGTCCTCACCATCATTTACCATGCCAGATCCACTTTAGGAGGGAGACTGC AGGCCAAGATGGCGTCCATGAAAATGTTCCAGATTCAGTTCCACACTGGGTTCGTGCCTCGAAACGCAAC CACTGTGAAATTTGCAAAGTATGACCTGGATGCTTGTGACATTCAAGAGAAGTACCCAGATCTGTTCCAG GTGAACCTGGAAGTGGAGGTGGAGCCTAGAGACAGGCCCAGCCGAGACGTTCCACCTTGGGAGAATACCA GCCTAAGGGGGTTAAACCCCAAGATCCTCTTTTCCAACAGGGAAGAGCAACAGGACATTCTGTCTAAGTT TGGGAAACCAGAGCTACCCCGGCAGCCGGGCTCCACAGCTCAGTATGATGCTGAGGCAGGGTCTCCAGAG GCTGAGATCACAGAGTCGGACTCACCACAGAGCAGCAGCACGGACACCAACCACTTTCTTCACACACTGG ATTGGCAGGAGGAAAAAGACCCAGAGACTGGTGTAGACAATACCTCTCCTAAGGAGAGTCAGTCTAACCT GATTGCAGATGGAGATGGAAGTGAAGTATCAGATGAAGAAGAGGCTTCATGCCCTAGTGAAGAGAGAAAG CCTGGGGCTGGAGAAGATACACCAAGGCTGGCAGCTGGGACCAGACAGCAAGACTTAATATTTGATGTGG GCATGCTGGCTGCCCCACAGGAGCCTGTACAGCCTGAAGAAGGTGTCGATCTCCTGGGGCTGCACTCTGA AGGGGACTTAAGGCCTGCTGCTCCCTTGCAGGCTAGCGGGGTCCAGTCTAGCAACACTGACCTGTTGAGC TCCCTTCTTGAACCATCTGATGCTTCTCAAGTGGGACCTCCTGGTGACCTGCTTGGTGGTGAGACTCCTC TGCTGTTAGCAAGCCCAGTTTCTCTTCTTGGGGTGCAGAGCAACCTGCAAGGAAAAGTCCCTGACACTGT GGACCCATTTGACCAGTTCCTGCTGCCATCCAGCTCGGACACCCAGCCCTGCTCCAAGCCTGATCTCTTT GGAGAGTTTCTCAACTCTGACTCTGTAGCTTCCTCAACTGCCTTCCCATCGACCCACAGCGCCCCACCCC CATCCTGCAGCACTGCCTTCCTGCACCTGGGAGATCTGCCAGCAGAGCCCAACAAGGTGATTGCTTCATC CAGCCACCCAGATCTGCTAGGAGGATGGGATACGTGGGCTGAGACTGCTCTACCTGGGCCGGCCTCCATG CCAGTACCAGAAGGTACTCTCTTCTCTTCTGCAGGTCACCCAGCCCCTCCAGGCCCCAACCCCAGCCAAA CCAAGTCTCAGAACCCAGACCCATTTGCTGACCTCAGTGATCTCAGCTCTAGCCTCCAAGGCTTGCCTGC TGGACTTCCTGCAGGGAGCTTCGTTGGCACATCAGCCACCACTCACAAAAGCAACAGCTCCTGGCAGACA ACTCGTCCCACAGCCCCTGGAACCTCATGGCCCCCGCAGGCCAAGCCAGCCCCCAGAGCCTCTGAACAAC TAAGGTCTCACTTTAGTGTGATTGGGGCCCGAGAAGAGAGAGGTGTCCGTGCGCCCAGCTTTGCCCAAAA GCCAAAGGTCTCAGAAAATGATTTTGAAGATCTGCTGCCTAATCAAGGCTTCTCTAAGTCTGACAAGAAG GGGCCAAAGACCATGGCAGAGATGCGGAAACAGGAACTTGCCAGAGATACAGACCCATTCAAATTGAAGC TTTTGGACTGGATTGAAGGCAAAGAGAGGAATATTCGTGCACTGCTGTCCACTCTGCACACAGTATTGTG GGATGGGGAGAGCCGCTGGACACCTGTGAGTATGGCTGACCTGGTGACTCCAGAGCAGGTGAAGAAGCAG TACCGCCGTGCAGTGCTGGTAGTGCATCCTGATAAGGCCACAGGGCAGCCATATGAACAGTCTGCCAAGA TGATCTTCATGGAGCTGAATGATGCATGGTCTGAGTTTGAAAACCAGGGCTCAAGGCCCCTCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031030
Insert Size	3918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031030.2, NP_112292.1
RefSeq Size	4453 bp
RefSeq ORF	3918 bp
Locus ID	81659
UniProt ID	P97874
Gene Summary	may be a serine/threonine protein kinase; may be involved with nucleotide metabolism [RGD, Feb 2006]

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