Gak (NM_031030) Rat Tagged ORF Clone
CAT#: RR215048
- TrueORF®
Gak (Myc-DDK-tagged ORF) - Rat cyclin G associated kinase (Gak), (10 ug)
"NM_031030" in other vectors (1)
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CNY 20,900.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR215048 representing NM_031030
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTGCTGCAGTCTGCACTGGACTTCCTGGCGGGCCCTGGTTCTCTTGGCGGAGCTGCCGGCCGTG ACCAGAGTGACTTCGTGGGGCAGACTGTGGAGCTGGGCGAGCTGCGTCTGCGGGTGCGGCGGGTCCTGGC CGAGGGAGGGTTTGCATTTGTTTATGAAGCTCAAGATCTGGGAAGTGGCAGAGAGTATGCATTAAAGAGA TTACTATCCAATGAAGAGGAAAAGAACAGAGCCATCATTCAGGAAGTATGTTTCTTGAAAAAACTTTCTG GCCACCCCAATATTGTCCAGTTCTGCTCTGCAGCATCCATAGGAAAAGAGGAATCGGACACTGGGCAGGC TGAGTTCCTCCTGCTTACGGAGCTCTGTAAAGGACAGCTGGTGGAGTTTCTCAGGAGAGTTGAATGTAAA GGCCCTCTATCCTGCGACAGCATTCTGAAGATCTTCTACCAGACATGCAGAGCAGTGCAGCACATGCACA GGCAGAAACCACCCATCATCCACAGGGATCTCAAGGTTGAAAACTTACTGCTTAGTAACCAGGGGACCAT TAAGCTGTGTGACTTTGGCAGTGCCACAACCATCTCCCATTATCCTGACTACAGCTGGAGCGCCCAGAAG CGAGCAATGGTGGAGGAAGAGATCACGAGGAACACCACACCCATGTACAGAACGCCAGAAATTGTAGACC TGTATTCTAACTTCCCTATTGGCGAAAAGCAGGATATCTGGGCACTGGGCTGTATCTTATACCTGCTGTG TTTCCGGCAGCATCCTTTTGAAGATGGAGCAAAACTTCGGATAGTCAATGGGAAGTATTCCATTCCTGTG AATGACACTCGTTACACAGTCTTCCATGACCTTATTCGTGGCATGCTAAAGGTCAATCCAGAAGAGAGGC TATCCATTGCTGAAGTTGTCCGACAACTGCAGGAAATTGCAGCAGCCCGGAATGTGAACCCCAAAGCCCC CATCACAGAACTTCTGGAGCAGAATGGTGGCTATGGGAACTCAGGGCCTTCCCGAGCACAACCACCTTCT GGGGGCCCTGTGAACAGCAGTGGAGTTTTGGCTCTGGCAGAGTATGACCAGCCCTATGGTGGGTTTCTCG ATATCCTACGGGGTGGGACAGAACGGCTCTTCACCAACCTCAAGGATACTTCCTCCAAGGTCATCCAGTC TGTGGCTAACTATGCAAAGGGCGATCTTGACATATCTTACATCACATCCAGGATTGCAGTGATGTCGTTC CCAGCAGAAGGTGTGGAGTCAGCAATCAAAAACAATATAGAGGATGTACGATTGTTTCTGGATGCCAAGC ATCCAGGACATTATGCTGTCTACAACCTTTCTCCAAGAATATACCGGGCTTCCAAGTTCCACAATCGGGT CACTGAGTGTGGCTGGGCAGTCAGGCGGGCACCACATCTCCACAGTTTGTATACTCTATGCAGGAGCATG CATGCCTGGCTCCGGGAAGACCACAGGAACGTCTGTGTTGTACATTGCATGGATGGGAGAGCTGCGTCTG CTGTGGCAGTCTGTGCATTCCTGTGCTTCTGCCGTCTCTTCAGCACTGCAGAGGCTGCTGTGTACATGTT CAGCATGAAGCGCTGCCCACCAGGCATTTGGCCATCCCACAAAAGGTACATTGAATACGTGTGTGACATG GTGGCAGAGGAACCCATCACGCCCCACAGCAAGCCAATGCTGGTGAAATCTGTTGTCATGACCCCTGTGC CACTGTTCAGCAAGCAGAGGAATGGCTGCCGACCATTCTGTGAGGTCTATGTTGGGGAGGAGCGTGTAAC CACCACATCCCAGGAGTATGACAGGATGAAGGAATTTAAAATTGAGGATGGCAAGGCTGTCATCCCCCTG GGCATAACAGTTCAAGGCGATGTCCTCACCATCATTTACCATGCCAGATCCACTTTAGGAGGGAGACTGC AGGCCAAGATGGCGTCCATGAAAATGTTCCAGATTCAGTTCCACACTGGGTTCGTGCCTCGAAACGCAAC CACTGTGAAATTTGCAAAGTATGACCTGGATGCTTGTGACATTCAAGAGAAGTACCCAGATCTGTTCCAG GTGAACCTGGAAGTGGAGGTGGAGCCTAGAGACAGGCCCAGCCGAGACGTTCCACCTTGGGAGAATACCA GCCTAAGGGGGTTAAACCCCAAGATCCTCTTTTCCAACAGGGAAGAGCAACAGGACATTCTGTCTAAGTT TGGGAAACCAGAGCTACCCCGGCAGCCGGGCTCCACAGCTCAGTATGATGCTGAGGCAGGGTCTCCAGAG GCTGAGATCACAGAGTCGGACTCACCACAGAGCAGCAGCACGGACACCAACCACTTTCTTCACACACTGG ATTGGCAGGAGGAAAAAGACCCAGAGACTGGTGTAGACAATACCTCTCCTAAGGAGAGTCAGTCTAACCT GATTGCAGATGGAGATGGAAGTGAAGTATCAGATGAAGAAGAGGCTTCATGCCCTAGTGAAGAGAGAAAG CCTGGGGCTGGAGAAGATACACCAAGGCTGGCAGCTGGGACCAGACAGCAAGACTTAATATTTGATGTGG GCATGCTGGCTGCCCCACAGGAGCCTGTACAGCCTGAAGAAGGTGTCGATCTCCTGGGGCTGCACTCTGA AGGGGACTTAAGGCCTGCTGCTCCCTTGCAGGCTAGCGGGGTCCAGTCTAGCAACACTGACCTGTTGAGC TCCCTTCTTGAACCATCTGATGCTTCTCAAGTGGGACCTCCTGGTGACCTGCTTGGTGGTGAGACTCCTC TGCTGTTAGCAAGCCCAGTTTCTCTTCTTGGGGTGCAGAGCAACCTGCAAGGAAAAGTCCCTGACACTGT GGACCCATTTGACCAGTTCCTGCTGCCATCCAGCTCGGACACCCAGCCCTGCTCCAAGCCTGATCTCTTT GGAGAGTTTCTCAACTCTGACTCTGTAGCTTCCTCAACTGCCTTCCCATCGACCCACAGCGCCCCACCCC CATCCTGCAGCACTGCCTTCCTGCACCTGGGAGATCTGCCAGCAGAGCCCAACAAGGTGATTGCTTCATC CAGCCACCCAGATCTGCTAGGAGGATGGGATACGTGGGCTGAGACTGCTCTACCTGGGCCGGCCTCCATG CCAGTACCAGAAGGTACTCTCTTCTCTTCTGCAGGTCACCCAGCCCCTCCAGGCCCCAACCCCAGCCAAA CCAAGTCTCAGAACCCAGACCCATTTGCTGACCTCAGTGATCTCAGCTCTAGCCTCCAAGGCTTGCCTGC TGGACTTCCTGCAGGGAGCTTCGTTGGCACATCAGCCACCACTCACAAAAGCAACAGCTCCTGGCAGACA ACTCGTCCCACAGCCCCTGGAACCTCATGGCCCCCGCAGGCCAAGCCAGCCCCCAGAGCCTCTGAACAAC TAAGGTCTCACTTTAGTGTGATTGGGGCCCGAGAAGAGAGAGGTGTCCGTGCGCCCAGCTTTGCCCAAAA GCCAAAGGTCTCAGAAAATGATTTTGAAGATCTGCTGCCTAATCAAGGCTTCTCTAAGTCTGACAAGAAG GGGCCAAAGACCATGGCAGAGATGCGGAAACAGGAACTTGCCAGAGATACAGACCCATTCAAATTGAAGC TTTTGGACTGGATTGAAGGCAAAGAGAGGAATATTCGTGCACTGCTGTCCACTCTGCACACAGTATTGTG GGATGGGGAGAGCCGCTGGACACCTGTGAGTATGGCTGACCTGGTGACTCCAGAGCAGGTGAAGAAGCAG TACCGCCGTGCAGTGCTGGTAGTGCATCCTGATAAGGCCACAGGGCAGCCATATGAACAGTCTGCCAAGA TGATCTTCATGGAGCTGAATGATGCATGGTCTGAGTTTGAAAACCAGGGCTCAAGGCCCCTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215048 representing NM_031030
Red=Cloning site Green=Tags(s) MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKR LLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLRRVECK GPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQK RAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPV NDTRYTVFHDLIRGMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPS GGPVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSF PAEGVESAIKNNIEDVRLFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSM HAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDM VAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPL GITVQGDVLTIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQ VNLEVEVEPRDRPSRDVPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPE AEITESDSPQSSSTDTNHFLHTLDWQEEKDPETGVDNTSPKESQSNLIADGDGSEVSDEEEASCPSEERK PGAGEDTPRLAAGTRQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQASGVQSSNTDLLS SLLEPSDASQVGPPGDLLGGETPLLLASPVSLLGVQSNLQGKVPDTVDPFDQFLLPSSSDTQPCSKPDLF GEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPNKVIASSSHPDLLGGWDTWAETALPGPASM PVPEGTLFSSAGHPAPPGPNPSQTKSQNPDPFADLSDLSSSLQGLPAGLPAGSFVGTSATTHKSNSSWQT TRPTAPGTSWPPQAKPAPRASEQLRSHFSVIGAREERGVRAPSFAQKPKVSENDFEDLLPNQGFSKSDKK GPKTMAEMRKQELARDTDPFKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQ YRRAVLVVHPDKATGQPYEQSAKMIFMELNDAWSEFENQGSRPLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031030 |
ORF Size | 3915 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031030.2, NP_112292.1 |
RefSeq Size | 4453 bp |
RefSeq ORF | 3918 bp |
Locus ID | 81659 |
UniProt ID | P97874 |
MW | 143.7 kDa |
Gene Summary | may be a serine/threonine protein kinase; may be involved with nucleotide metabolism [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN215048 | Gak (untagged ORF) - Rat cyclin G associated kinase (Gak), (10 ug) |
CNY 20,900.00 |