Nalcn (NM_153630) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Vgcnl1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215561 representing NM_153630 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAAAAGAAAGCAGAGTTCCAGGGTGGAAGCCCAGCCAGTAACTGACTTTGGTCCTGACGAATCTC TGTCAGACAATGCTGACATACTCTGGATTAATAAGCCATGGGTGCACTCTCTGCTGCGCATCTGTGCCAT CATCAGCGTCATCTCAGTGTGCATGAACACACCTATGACCTTCGAGCACTATCCTCCCCTTCAGTATGTG ACCTTCACCCTGGACACTTTACTGATGTTTCTCTACACTGCAGAGATGATAGCAAAGATGCACATACGGG GAATTGTCAAGGGCGATAGCTCCTATGTGAAGGACCGCTGGTGTGTTTTTGATGGATTCATGGTCTTTTG CCTTTGGGTTTCTCTCGTGTTACAGGTGTTTGAAATAGCTGACATAGTTGATCAGATGTCACCTTGGGGC ATGCTGCGGATCCCACGGCCACTCATTATGATCCGGGCTTTCAGGATTTATTTCCGATTCGAACTGCCAA GGACCCGAATTACAAACATTTTAAAGCGGTCAGGAGAACAAATATGGAGTGTCTCAATCTTCCTCCTCTT CTTCCTTCTTCTGTATGGGATTTTAGGAGTTCAGATGTTTGGAACATTTACCTACCACTGCGTAGTCAAT GACACAAAGCCAGGGAATGTAACCTGGAATAGCTTAGCTATCCCAGATACGCACTGCTCGCCAGAGCTAG AAGAAGGCTATCAGTGCCCCCCAGGATTTAAATGCATGGACCTGGAAGACCTGGGACTTAGCAGGCAAGA GCTGGGCTACAGTGGCTTTAATGAGATAGGCACGAGTATATTCACAGTCTATGAGGCTTCATCTCAGGAA GGCTGGGTATTCCTCATGTACAGAGCAATCGACAGTTTCCCCCGTTGGCGTTCCTACTTCTACTTCATCA CGCTGATTTTCTTCCTTGCCTGGCTTGTCAAGAATGTGTTCATTGCTGTCATCATTGAGACATTTGCAGA AATCAGAGTACAATTTCAACAAATGTGGGGAACTCGGAGCAGCACAACTTCCACTGCCACCACGCAGATG TTCCATGAAGATGCTGCCGGTGGCTGGCAGCTGGTAGCTGTGGATGTCAACAAGCCCCAGGGACGTGCCC CAGCCTGCCTACAGAAAATGATGCGGTCATCAGTTTTCCACATGTTTATCCTGAGCATGGTGACTGTGGA TGTAATAGTTGCTGCCAGCAACTACTACAAGGGAGAGAACTTCAGAAGGCAGTATGACGAGTTCTACCTT GCAGAGGTGGCTTTTACAGTTCTTTTTGATCTGGAAGCACTTCTGAAGATATGGTGTTTGGGGTTTACTG GCTACATCAGCTCATCTCTCCACAAGTTTGAACTACTTCTGGTTATTGGGACAACTCTCCATGTATACCC TGATCTTTATCATTCTCAATTTACCTACTTCCAGGTTCTTCGGGTAGTCCGGCTTATTAAGATTTCCCCA GCATTGGAAGATTTTGTGTACAAGATATTTGGTCCCGGGAAAAAACTTGGAAGCTTGGTGGTGTTCACTG CCAGTCTCCTGATAGTTATGTCCGCCATCAGTTTGCAGATGTTCTGCTTCGTCGAAGAACTGGACAGATT CACCACATTTCCAAGGGCATTTATGTCCATGTTCCAGATCCTCACCCAGGAAGGATGGGTGGATGTGATG GATCAGACTCTGAATGCTGTGGGGCATATGTGGGCTCCACTGGTTGCCATCTATTTCATCCTCTACCATC TCTTTGCAACGCTGATCCTCCTGAGTTTGTTTGTTGCTGTTATTTTGGACAACTTAGAACTTGATGAAGA TCTAAAGAAGCTCAAACAATTAAAACAAAGTGAAGCGAACGCAGATACCAAAGAAAAACTTCCTTTGCGC TTGAGAATCTTCGAAAAATTCCCAAACAGACCGCAAATGGTGAAAATCTCAAAACTTCCTTCAGATTTTA CAGTTCCTAAGATCAGGGAAAGCTTCATGAAGCAGTTCATTGACCGCCAGCAACAGGACACCTGCTGTCT CTTCAGAATTCTCCCCTCTACCTCTTCCTCATCCTGTGACAACCCCAAGCGGCCCACAGTTGAAGACAAC AAATACATTGATCAAAAACTCCGCAAGTCTGTTTTCAGCATAAGGGCAAGGAACCTTCTGGAAAAGGAGA CCGCAGTCACAAAAATCTTAAGAGCTTGCACTCGACAGCGCATGCTGAGCGGATCATTCGAGGGGCAGCC AGCAAAGGAGAGGTCGATCCTCAGCGTGCAGCATCACATCCGCCAGGAGCGCAGGTCACTCAGACATGGA TCCAACAGCCAGAGGATCAGCAGGGGCAAATCTCTTGAAACGTTAACTCAAGATCATTCCAATACGGTGC GCTACAGAAATGCACAAAGAGAAGACAGTGAAATAAAGATGATCCAGGAGAAGAAGGAGCAAGCAGAGAT GAAAAGGAAGGTGCAAGAAGAGGAGCTGCGAGAGAACCACCCATACTTTGACAAGCCTCTCTTCATCGTG GGTCGAGAACACAGGTTCAGAAACTTCTGCCGCGTGGTGGTTCGAGCACGCTTCAATGCATCCAAAACAG ACCCTGTCACGGGAGCTGTGAAAAATACAAAGTACCACCAACTTTATGATTTGCTGGGACTTGTCACCTA CCTGGACTGGGTCATGATCACTGTAACCATCTGCTCTTGCATTTCTATGATGTTCGAATCCCCCTTCCGG AGAGTCATGCATGCACCCACTTTACAGATCGCTGAATATGTGTTTGTGATATTCATGAGCATTGAGCTTA ATCTGAAGATTATGGCAGATGGCTTGTTTTTCACTCCAACTGCTGTCATCAGGGACTTTGGTGGCGTCAT GGACATATTTATCTATCTTGTGAGCTTGATATTTCTTTGTTGGATGCCTCAAAATGTGCCTGCTGAGTCA GGAGCCCAGCTCCTGATGGTTCTTCGGTGCCTAAGACCTCTTCGGATATTCAAACTGGTGCCACAAATGA GGAAAGTTGTTCGAGAACTTTTCAGTGGTTTCAAGGAAATATTTTTGGTCTCCATTCTGTTGCTGACACT AATGCTTGTTTTTGCAAGCTTTGGTGTTCAGCTCTTTGCCGGAAAGCTAGCCAAGTGCAATGACCCCAAC ATTATCAGAAGGGAAGACTGTAACGGCATCTTCAGAATTAATGTAAGTGTGTCCAAGAACTTAAATTTAA AACTAAGACCTGGAGAGAAAAAACCTGGATTTTGGGTGCCCCGTGTTTGGGCAAATCCTCGTAACTTTAA CTTTGACAATGTGGGAAATGCTATGCTGGCATTGTTTGAAGTTCTGTCCTTGAAAGGCTGGGTAGAAGTG AGAGATGTCATTATTCATCGTGTGGGGCCGATCCATGGAATCTATATTCATGTTTTCGTATTCCTGGGTT GCATGATTGGACTGACTCTTTTTGTCGGTGTAGTTATTGCTAACTTCAATGAAAACAAGGGGACAGCCCT GCTGACGGTAGATCAGAGAAGATGGGAAGATCTCAAGAGCAGATTGAAGATCGCACAGCCTCTTCATCTC CCACCTCGGCCGGATAATGATGGATTTAGAGCTAAAATGTATGACATAACCCAGCATCCGTTTTTTAAAA GGACAATTGCGTTGCTGGTTCTGGCCCAGTCTGTGTTGCTATCTGTCAAGTGGGATGTTGAGGATCCTGT GACAGTTCCTTTGGCAACCATGTCGGTCGTGTTCACCTTCATCTTTGTCTTAGAGGTTACAATGAAGATC ATAGCAATGTCACCAGCTGGTTTCTGGCAAAGCAGAAGAAACCGATATGATCTCTTGGTGACGTCTCTTG GTGTTGTGTGGGTGGTGCTCCATTTTGCTCTGCTGAATGCATACACCTACATGATGGGAGCCTGTGTGAT TGTCTTTAGATTTTTCTCCATCTGTGGAAAGCATGTGACATTAAAGATGCTCCTTTTGACTGTGGTTGTC AGCATGTACAAGAGCTTCTTTATCATCGTAGGAATGTTTCTCTTGCTGCTGTGCTATGCCTTTGCTGGGG TGGTTCTGTTTGGCACTGTAAAGTATGGAGAGAACATTAACAGGCATGCCAATTTTTCTTCAGCTGGCAA AGCCATTACTGTATTGTTCCGAATTGTCACAGGGGAAGACTGGAATAAGATTATGCATGATTGTATGGTT CAGCCTCCATTTTGTACTCCTGATGAATTTACATATTGGGCAACAGACTGTGGCAATTATGCAGGGGCAC TTATGTACTTCTGCTCATTCTATGTCATCATCGCCTACATCATGCTGAATCTACTTGTAGCCATAATTGT GGAGAATTTCTCTTTGTTTTATTCCACTGAAGAGGACCAGCTTTTGAGTTACAATGATCTTCGCCATTTT CAAATCATATGGAATATGGTAGATGATAACGAGAGGTGTGATCCCACCTTCCGAGTGAAGTTCCTGCTAC GGCTACTGCGTGGGAGGCTAGAAGTGGACCTTGATAAAGACAAGCTCCTGTTTAAGCACATGTGCTATGA GATGGAGAGGCTGCACAATGGTGGTGATGTCACCTTCCATGATGTCTTGAGCATGCTGTCCTACCGCTCT GTAGACATCAGGAAGAGCCTGCAGCTAGAGGAACTGCTGGCAAGGGAGCAGCTGGAGTACACCATAGAGG AGGAGGTGGCTAAGCAGACTATTCGCATGTGGCTGAAGAAGTGCTTAAAACGCATCCGGGCTAAACAGCA GCAGTCATGCAGCATCATCCACAGCCTGAGAGAGAGCCAGCAGCAAGAGTTGAGCCGATTCCTGAATCCT CCCAGCATTGAGACCACCCAACCAAGCGAAGATACCAACGCCAACAGCCAGGACCACAATACGCAACCTG AGTCAAGCAGTCAGCAGCAGCTCTTGAGCCCTACTCTGTCAGACAGAGGAGGAAGTCGACAGGATGCAGC AGATACTGGGAAACCCCAAAGAAAGATTGGGCAATGGCGTCTGCCCTCAGCTCCCAAACCAATAAGCCAT TCTGTGTCTTCGGTTAACCTACGGTTTGGAGGAAGGACAACAATGAAGTCTGTGGTGTGCAAGATGAACC CCATGCCAGACACAGCTTCCTGTGGCTCCGAAGTTAAAAAGTGGTGGACCAGACAGCTGACTGTGGAGAG TGACGAGAGTGGAGATGACCTCCTAGATATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_153630
Insert Size	5214 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_153630.1, NP_705894.1
RefSeq Size	6779 bp
RefSeq ORF	5214 bp
Locus ID	266760
UniProt ID	Q6Q760
Gene Summary	may act as a voltage activated cation channel [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.